Gene description for WRB
Gene name tryptophan rich basic protein
Gene symbol WRB
Other names/aliases CHD5
Species Homo sapiens
 Database cross references - WRB
ExoCarta ExoCarta_7485
Vesiclepedia VP_7485
Entrez Gene 7485
HGNC 12790
MIM 602915
UniProt O00258  
 WRB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WRB
Molecular Function
    protein binding GO:0005515 IPI
    protein-membrane adaptor activity GO:0043495 IBA
Biological Process
    protein insertion into ER membrane GO:0045048 IDA
    protein insertion into ER membrane GO:0045048 IMP
    protein insertion into ER membrane GO:0045048 NAS
    protein stabilization GO:0050821 IDA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IBA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IDA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IMP
Subcellular Localization
    nucleus GO:0005634 TAS
    endoplasmic reticulum membrane GO:0005789 NAS
    GET complex GO:0043529 IBA
    GET complex GO:0043529 IDA
    GET complex GO:0043529 IPI
 Experiment description of studies that identified WRB in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WRB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SERPINA6  
Affinity Capture-MS Homo sapiens
3 TOR3A 64222
Affinity Capture-MS Homo sapiens
4 AQP1 358
Two-hybrid Homo sapiens
5 CLCN7 1186
Two-hybrid Homo sapiens
6 SLC15A1  
Affinity Capture-MS Homo sapiens
7 APLNR  
Affinity Capture-MS Homo sapiens
8 VAPA 9218
Affinity Capture-MS Homo sapiens
9 RASGRP3  
Two-hybrid Homo sapiens
10 TTYH1  
Affinity Capture-MS Homo sapiens
11 C14orf1 11161
Two-hybrid Homo sapiens
12 TXNDC15  
Affinity Capture-MS Homo sapiens
13 FAS 355
Affinity Capture-MS Homo sapiens
14 CAMLG 819
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
15 CMTM8  
Affinity Capture-MS Homo sapiens
16 ORMDL3 94103
Two-hybrid Homo sapiens
17 LPAR6  
Affinity Capture-MS Homo sapiens
18 CD53 963
Two-hybrid Homo sapiens
19 HMOX2 3163
Two-hybrid Homo sapiens
20 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 DPEP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ERGIC3 51614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PTPN9 5780
Two-hybrid Homo sapiens
24 ITGB8 3696
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 HTATIP2 10553
Two-hybrid Homo sapiens
26 EGFR 1956
Negative Genetic Homo sapiens
27 SPTSSA  
Two-hybrid Homo sapiens
28 TMEM42  
Two-hybrid Homo sapiens
29 CLPP 8192
Proximity Label-MS Homo sapiens
30 CCL4L2  
Two-hybrid Homo sapiens
31 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DUSP6 1848
Affinity Capture-MS Homo sapiens
33 RIT1 6016
Negative Genetic Homo sapiens
34 ASNA1 439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SLC18A2  
Affinity Capture-MS Homo sapiens
36 SERP2  
Two-hybrid Homo sapiens
37 TM4SF19  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 APOL2 23780
Two-hybrid Homo sapiens
39 SLC3A2 6520
Affinity Capture-MS Homo sapiens
40 APOA2 336
Two-hybrid Homo sapiens
41 LRRC55  
Affinity Capture-MS Homo sapiens
42 MSH5  
Affinity Capture-MS Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 PPAPDC1A  
Two-hybrid Homo sapiens
45 RTP2  
Two-hybrid Homo sapiens
46 COX20  
Two-hybrid Homo sapiens
47 SLC35E3  
Two-hybrid Homo sapiens
48 KRAS 3845
Negative Genetic Homo sapiens
49 C9orf72  
Affinity Capture-MS Homo sapiens
50 CD302 9936
Two-hybrid Homo sapiens
51 SLC22A4 6583
Affinity Capture-MS Homo sapiens
52 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CNDP1  
Affinity Capture-MS Homo sapiens
54 SLC7A1 6541
Two-hybrid Homo sapiens
55 STX2 2054
Two-hybrid Homo sapiens
56 VCP 7415
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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