Gene description for ITGB8
Gene name integrin, beta 8
Gene symbol ITGB8
Other names/aliases -
Species Homo sapiens
 Database cross references - ITGB8
ExoCarta ExoCarta_3696
Vesiclepedia VP_3696
Entrez Gene 3696
HGNC 6163
MIM 604160
UniProt P26012  
 ITGB8 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for ITGB8
Molecular Function
    integrin binding GO:0005178 IBA
    metal ion binding GO:0046872 IEA
    extracellular matrix protein binding GO:1990430 IDA
Biological Process
    vasculogenesis GO:0001570 IMP
    ganglioside metabolic process GO:0001573 IEA
    immune response GO:0006955 IEA
    cell adhesion GO:0007155 TAS
    cell-matrix adhesion GO:0007160 IMP
    cell-matrix adhesion GO:0007160 NAS
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 IDA
    response to virus GO:0009615 IEA
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of gene expression GO:0010629 IMP
    cell migration GO:0016477 IBA
    cell adhesion mediated by integrin GO:0033627 IBA
    positive regulation of angiogenesis GO:0045766 IMP
    cartilage development GO:0051216 IMP
    hard palate development GO:0060022 IEA
    placenta blood vessel development GO:0060674 TAS
    Langerhans cell differentiation GO:0061520 IEA
    cell-cell adhesion GO:0098609 IBA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IBA
    integrin complex GO:0008305 IBA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    integrin alphav-beta8 complex GO:0034686 IDA
    integrin alphav-beta8 complex GO:0034686 IPI
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ITGB8 in exosomes
1
Experiment ID 259
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ITGB8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TARSL2 123283
Affinity Capture-MS Homo sapiens
2 SLC25A16  
Affinity Capture-MS Homo sapiens
3 RPL23 9349
Affinity Capture-MS Homo sapiens
4 PAK3  
Affinity Capture-MS Homo sapiens
5 KDELC1 79070
Affinity Capture-MS Homo sapiens
6 NME4 4833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TREX1  
Affinity Capture-MS Homo sapiens
8 TYROBP  
Affinity Capture-MS Homo sapiens
9 EDC3 80153
Affinity Capture-MS Homo sapiens
10 LRRC32 2615
Affinity Capture-MS Homo sapiens
11 DDX58 23586
Affinity Capture-RNA Homo sapiens
12 RHEB 6009
Affinity Capture-MS Homo sapiens
13 L2HGDH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RAP1B 5908
Affinity Capture-MS Homo sapiens
15 B3GNT2 10678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LMF1 64788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 WRB 7485
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 MICB  
Affinity Capture-MS Homo sapiens
19 TGFB1 7040
Affinity Capture-MS Homo sapiens
20 C19orf52  
Affinity Capture-MS Homo sapiens
21 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 RHBDL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PARL  
Affinity Capture-MS Homo sapiens
24 ACTR3C 653857
Affinity Capture-MS Homo sapiens
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