Gene description for ITGB8
Gene name integrin, beta 8
Gene symbol ITGB8
Other names/aliases -
Species Homo sapiens
 Database cross references - ITGB8
ExoCarta ExoCarta_3696
Entrez Gene 3696
HGNC 6163
MIM 604160
UniProt P26012  
 ITGB8 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for ITGB8
Molecular Function
    receptor binding GO:0005102 IEA
    extracellular matrix protein binding GO:1990430 IDA
    receptor activity GO:0004872 IEA
Biological Process
    ganglioside metabolic process GO:0001573 IEA
    negative regulation of gene expression GO:0010629 IMP
    placenta blood vessel development GO:0060674 TAS
    integrin-mediated signaling pathway GO:0007229 IEA
    cell-matrix adhesion GO:0007160 IMP
    cell adhesion GO:0007155 TAS
    positive regulation of gene expression GO:0010628 IMP
    extracellular matrix organization GO:0030198 TAS
    cartilage development GO:0051216 IMP
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    integrin complex GO:0008305 TAS
    cell surface GO:0009986 IDA
    plasma membrane GO:0005886 TAS
    integrin alphav-beta8 complex GO:0034686 IDA
 Experiment description of studies that identified ITGB8 in exosomes
1
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ITGB8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFB1 7040
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ITGB8 is involved
PathwayEvidenceSource
Integrin cell surface interactions TAS Reactome
Molecules associated with elastic fibres TAS Reactome





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