Gene description for EDC3
Gene name enhancer of mRNA decapping 3
Gene symbol EDC3
Other names/aliases LSM16
YJDC
YJEFN2
Species Homo sapiens
 Database cross references - EDC3
ExoCarta ExoCarta_80153
Entrez Gene 80153
HGNC 26114
MIM 609842
UniProt Q96F86  
 EDC3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for EDC3
Molecular Function
    RNA binding GO:0003723 IEA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
Biological Process
    exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay GO:0043928 TAS
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    gene expression GO:0010467 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    cytoplasmic mRNA processing body GO:0000932 IEA
 Experiment description of studies that identified EDC3 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EDC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAQ 10971
Affinity Capture-MS Homo sapiens
2 YWHAG 7532
Affinity Capture-MS Homo sapiens
3 YWHAB 7529
Affinity Capture-MS Homo sapiens
4 YWHAZ 7534
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EDC3 is involved
PathwayEvidenceSource
mRNA decay by 5' to 3' exoribonuclease TAS Reactome





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