Gene description for DCLK1
Gene name doublecortin-like kinase 1
Gene symbol DCLK1
Other names/aliases CL1
CLICK1
DCAMKL1
DCDC3A
DCLK
Species Homo sapiens
 Database cross references - DCLK1
ExoCarta ExoCarta_9201
Vesiclepedia VP_9201
Entrez Gene 9201
HGNC 2700
MIM 604742
UniProt O15075  
 DCLK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for DCLK1
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    neuron migration GO:0001764 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    nervous system development GO:0007399 TAS
    central nervous system development GO:0007417 TAS
    response to virus GO:0009615 IEP
    endosomal transport GO:0016197 NAS
    central nervous system projection neuron axonogenesis GO:0021952 IEA
    forebrain development GO:0030900 IEA
    protein localization to nucleus GO:0034504 IBA
    intracellular signal transduction GO:0035556 IEA
    axon extension GO:0048675 IBA
    dendrite morphogenesis GO:0048813 IEA
    regulation of postsynapse assembly GO:0150052 IEA
    negative regulation of protein localization to nucleus GO:1900181 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 TAS
    postsynaptic density GO:0014069 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified DCLK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DCLK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LARP1 23367
Affinity Capture-MS Homo sapiens
2 DENND4C 55667
Affinity Capture-MS Homo sapiens
3 LRFN1  
Affinity Capture-MS Homo sapiens
4 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 EFHC2  
Two-hybrid Homo sapiens
6 LIMA1 51474
Affinity Capture-MS Homo sapiens
7 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLC4A1AP  
Co-fractionation Homo sapiens
9 KLHL15  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 GIGYF2 26058
Affinity Capture-MS Homo sapiens
11 STARD13 90627
Affinity Capture-MS Homo sapiens
12 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
13 FAM53C 51307
Affinity Capture-MS Homo sapiens
14 UBE2H 7328
Affinity Capture-MS Homo sapiens
15 KSR1  
Affinity Capture-MS Homo sapiens
16 KIAA1804  
Affinity Capture-MS Homo sapiens
17 RTKN 6242
Affinity Capture-MS Homo sapiens
18 DENND1A 57706
Affinity Capture-MS Homo sapiens
19 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
20 AGAP1  
Affinity Capture-MS Homo sapiens
21 PPM1H  
Affinity Capture-MS Homo sapiens
22 MELK  
Affinity Capture-MS Homo sapiens
23 INPP5E 56623
Affinity Capture-MS Homo sapiens
24 CDK16 5127
Affinity Capture-MS Homo sapiens
25 SH3RF3  
Affinity Capture-MS Homo sapiens
26 RPTOR 57521
Affinity Capture-MS Homo sapiens
27 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CDC25B 994
Affinity Capture-MS Homo sapiens
29 KIF13B 23303
Affinity Capture-MS Homo sapiens
30 SRGAP2 23380
Affinity Capture-MS Homo sapiens
31 ANKRD34A  
Affinity Capture-MS Homo sapiens
32 USP21  
Affinity Capture-MS Homo sapiens
33 RAB3IP  
Affinity Capture-MS Homo sapiens
34 CAMSAP2  
Affinity Capture-MS Homo sapiens
35 NADK 65220
Affinity Capture-MS Homo sapiens
36 EDC3 80153
Affinity Capture-MS Homo sapiens
37 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
38 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
39 GAB2 9846
Affinity Capture-MS Homo sapiens
40 EIF4E2  
Affinity Capture-MS Homo sapiens
41 TESK2  
Affinity Capture-MS Homo sapiens
42 APP 351
Reconstituted Complex Homo sapiens
43 SYDE1 85360
Affinity Capture-MS Homo sapiens
44 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
45 NF1 4763
Affinity Capture-MS Homo sapiens
46 CDC25C  
Affinity Capture-MS Homo sapiens
47 SH3BP4 23677
Affinity Capture-MS Homo sapiens
48 KIF1C 10749
Affinity Capture-MS Homo sapiens
49 HDAC7  
Affinity Capture-MS Homo sapiens
50 CBY1  
Affinity Capture-MS Homo sapiens
51 MLLT4 4301
Affinity Capture-MS Homo sapiens
52 CGN  
Affinity Capture-MS Homo sapiens
53 DENND4A 10260
Affinity Capture-MS Homo sapiens
54 ZBTB21  
Affinity Capture-MS Homo sapiens
55 GIGYF1  
Affinity Capture-MS Homo sapiens
56 MAGI1  
Affinity Capture-MS Homo sapiens
57 GPM6A 2823
Affinity Capture-MS Homo sapiens
58 ZNF638 27332
Affinity Capture-MS Homo sapiens
59 LPIN3 64900
Affinity Capture-MS Homo sapiens
60 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 DCTN1 1639
Proximity Label-MS Homo sapiens
62 FAM110A  
Affinity Capture-MS Homo sapiens
63 FBLIM1 54751
Affinity Capture-MS Homo sapiens
64 DCX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 MAPRE3  
Proximity Label-MS Homo sapiens
66 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 MYC  
Affinity Capture-MS Homo sapiens
68 MAST3  
Affinity Capture-MS Homo sapiens
69 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
70 TIAM1  
Affinity Capture-MS Homo sapiens
71 PHLDB2 90102
Affinity Capture-MS Homo sapiens
72 HDAC4  
Affinity Capture-MS Homo sapiens
73 KCTD3  
Affinity Capture-MS Homo sapiens
74 FAM110B  
Affinity Capture-MS Homo sapiens
75 SRSF12  
Affinity Capture-MS Homo sapiens
76 RASAL2 9462
Affinity Capture-MS Homo sapiens
77 NAV1 89796
Affinity Capture-MS Homo sapiens
78 TBC1D25  
Affinity Capture-MS Homo sapiens
79 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
80 PTPN14 5784
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DCLK1 is involved
No pathways found





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