Gene description for DENND4A
Gene name DENN/MADD domain containing 4A
Gene symbol DENND4A
Other names/aliases IRLB
MYCPBP
Species Homo sapiens
 Database cross references - DENND4A
ExoCarta ExoCarta_10260
Entrez Gene 10260
HGNC 24321
MIM 600382
UniProt Q7Z401  
 DENND4A identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DENND4A
Molecular Function
    Rab guanyl-nucleotide exchange factor activity GO:0017112 IDA
    DNA binding GO:0003677 NAS
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    regulation of transcription, DNA-templated GO:0006355 NAS
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
 Experiment description of studies that identified DENND4A in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DENND4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAG 7532
Affinity Capture-MS Homo sapiens
2 YWHAQ 10971
Affinity Capture-MS Homo sapiens
3 YWHAB 7529
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DENND4A is involved
No pathways found





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