Gene description for PCBD1
Gene name pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Gene symbol PCBD1
Other names/aliases DCOH
PCBD
PCD
PHS
Species Homo sapiens
 Database cross references - PCBD1
ExoCarta ExoCarta_5092
Vesiclepedia VP_5092
Entrez Gene 5092
HGNC 8646
MIM 126090
UniProt P61457  
 PCBD1 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PCBD1
Molecular Function
    transcription coactivator activity GO:0003713 TAS
    phenylalanine 4-monooxygenase activity GO:0004505 IEA
    protein binding GO:0005515 IPI
    4-alpha-hydroxytetrahydrobiopterin dehydratase activity GO:0008124 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    tetrahydrobiopterin biosynthetic process GO:0006729 IEA
    tyrosine biosynthetic process, by oxidation of phenylalanine GO:0019293 IEA
    positive regulation of DNA-templated transcription GO:0045893 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PCBD1 in exosomes
1
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCBD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PHF6  
Affinity Capture-MS Homo sapiens
2 ZNF202  
Two-hybrid Homo sapiens
3 CSRP2BP  
Affinity Capture-MS Homo sapiens
4 TRIM5 85363
Two-hybrid Homo sapiens
5 BRD7  
Two-hybrid Homo sapiens
6 YEATS2  
Affinity Capture-MS Homo sapiens
7 PAH 5053
Two-hybrid Homo sapiens
8 CUL1 8454
Affinity Capture-MS Homo sapiens
9 ZNF23  
Two-hybrid Homo sapiens
10 GTF3C3 9330
Two-hybrid Homo sapiens
11 SDCBP 6386
Two-hybrid Homo sapiens
12 PCBD2 84105
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 ZNF714  
Two-hybrid Homo sapiens
14 ADSS 159
Co-fractionation Homo sapiens
15 TFF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 NIF3L1 60491
Two-hybrid Homo sapiens
17 MIS18BP1  
Two-hybrid Homo sapiens
18 DVL2 1856
Two-hybrid Homo sapiens
19 POLR3A 11128
Two-hybrid Homo sapiens
20 PRNP 5621
Affinity Capture-MS Homo sapiens
21 DYRK1B  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
22 WDR1 9948
Co-fractionation Homo sapiens
23 WWP1 11059
Two-hybrid Homo sapiens
24 MBIP  
Affinity Capture-MS Homo sapiens
25 CACNA1C  
Two-hybrid Homo sapiens
26 BBS1 582
Affinity Capture-MS Homo sapiens
27 HNF1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SMARCD1 6602
Two-hybrid Homo sapiens
29 CUL5 8065
Affinity Capture-MS Homo sapiens
30 SUPT5H 6829
Two-hybrid Homo sapiens
31 CUL3 8452
Affinity Capture-MS Homo sapiens
32 KANK2 25959
Two-hybrid Homo sapiens
33 ZZZ3  
Affinity Capture-MS Homo sapiens
34 CALCOCO1 57658
Two-hybrid Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 CUL4B 8450
Affinity Capture-MS Homo sapiens
37 UBB 7314
Affinity Capture-MS Homo sapiens
38 PAPOLG  
Two-hybrid Homo sapiens
39 DNAJB3 414061
Affinity Capture-MS Homo sapiens
40 KLF13  
Two-hybrid Homo sapiens
41 PTPDC1  
Affinity Capture-MS Homo sapiens
42 PSMA1 5682
Two-hybrid Homo sapiens
43 ACIN1 22985
Reconstituted Complex Homo sapiens
44 SAP30 8819
Two-hybrid Homo sapiens
45 CDK5RAP2 55755
Two-hybrid Homo sapiens
46 NFKB1 4790
Two-hybrid Homo sapiens
47 HNF4A  
Two-hybrid Homo sapiens
48 HES4  
Two-hybrid Homo sapiens
49 ZNF195  
Two-hybrid Homo sapiens
50 GPCPD1  
Affinity Capture-MS Homo sapiens
51 ZMYM6  
Affinity Capture-MS Homo sapiens
52 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
53 TADA2A  
Affinity Capture-MS Homo sapiens
54 MYC  
Dosage Lethality Homo sapiens
55 NUDT18  
Two-hybrid Homo sapiens
56 FXR2 9513
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
57 WDYHV1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 CCDC102A 92922
Affinity Capture-MS Homo sapiens
59 COPS5 10987
Affinity Capture-MS Homo sapiens
60 ZNF567  
Two-hybrid Homo sapiens
61 RBL2 5934
Two-hybrid Homo sapiens
62 DENND4A 10260
Two-hybrid Homo sapiens
63 GORASP2 26003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
64 AFMID  
Co-fractionation Homo sapiens
65 ZNF263  
Two-hybrid Homo sapiens
66 UAP1L1 91373
Co-fractionation Homo sapiens
67 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
68 ZBTB25  
Two-hybrid Homo sapiens
69 PCBD1 5092
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 EHMT1  
Two-hybrid Homo sapiens
71 Arhgap18  
Affinity Capture-MS Mus musculus
72 HNF1B  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
73 C9orf72  
Affinity Capture-MS Homo sapiens
74 SCMH1  
Two-hybrid Homo sapiens
75 RPS24 6229
Reconstituted Complex Homo sapiens
76 ZNF451 26036
Two-hybrid Homo sapiens
77 TIMELESS  
Two-hybrid Homo sapiens
78 LNX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 DIP2C 22982
Two-hybrid Homo sapiens
80 CUL4A 8451
Affinity Capture-MS Homo sapiens
81 LACTB 114294
Affinity Capture-MS Homo sapiens
82 SOWAHC  
Two-hybrid Homo sapiens
83 CEBPZ  
Two-hybrid Homo sapiens
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