Gene description for WWP1
Gene name WW domain containing E3 ubiquitin protein ligase 1
Gene symbol WWP1
Other names/aliases AIP5
Tiul1
hSDRP1
Species Homo sapiens
 Database cross references - WWP1
ExoCarta ExoCarta_11059
Vesiclepedia VP_11059
Entrez Gene 11059
HGNC 17004
MIM 602307
UniProt Q9H0M0  
 WWP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for WWP1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    signal transduction GO:0007165 NAS
    central nervous system development GO:0007417 NAS
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 TAS
    monoatomic ion transmembrane transport GO:0034220 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    negative regulation of DNA-templated transcription GO:0045892 ISS
    symbiont entry into host cell GO:0046718 TAS
Subcellular Localization
    ubiquitin ligase complex GO:0000151 NAS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    extracellular exosome GO:0070062 HDA
    anchoring junction GO:0070161 IEA
 Experiment description of studies that identified WWP1 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WWP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 Mtx1  
Affinity Capture-MS Mus musculus
4 AXL 558
Affinity Capture-MS Homo sapiens
5 FLOT2 2319
Affinity Capture-MS Homo sapiens
6 KLF2 10365
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
7 RECQL5  
Affinity Capture-MS Homo sapiens
8 SCNN1A  
Two-hybrid Homo sapiens
9 TRIML1  
Affinity Capture-MS Homo sapiens
10 MC1R 4157
Affinity Capture-MS Homo sapiens
11 BCLAF1 9774
Affinity Capture-MS Homo sapiens
12 WWP1 11059
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
13 RPL30 6156
Affinity Capture-MS Homo sapiens
14 NME7 29922
Affinity Capture-MS Homo sapiens
15 VPRBP 9730
Affinity Capture-MS Homo sapiens
16 TRIM66  
Affinity Capture-MS Homo sapiens
17 TP63  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
18 SMAD4  
Affinity Capture-Western Homo sapiens
19 RPL10 6134
Affinity Capture-MS Homo sapiens
20 USP9X 8239
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
21 FAM189B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 UBC 7316
Biochemical Activity Homo sapiens
23 FAM168A  
Affinity Capture-MS Homo sapiens
24 DVL3 1857
Affinity Capture-Western Homo sapiens
25 ELAVL1 1994
Affinity Capture-MS Homo sapiens
26 ATN1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 RTN4 57142
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 LRP10 26020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FAU 2197
Affinity Capture-MS Homo sapiens
30 AMOTL1 154810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 URI1  
Affinity Capture-MS Homo sapiens
32 RPS11 6205
Affinity Capture-MS Homo sapiens
33 RPL10A 4736
Affinity Capture-MS Homo sapiens
34 RPL28 6158
Affinity Capture-MS Homo sapiens
35 BRCC3 79184
Affinity Capture-MS Homo sapiens
36 NONO 4841
Affinity Capture-MS Homo sapiens
37 ADAM9 8754
Affinity Capture-MS Homo sapiens
38 ZNF638 27332
Two-hybrid Homo sapiens
39 POLR2I 5438
Affinity Capture-MS Homo sapiens
40 PCBD1 5092
Two-hybrid Homo sapiens
41 RPL24 6152
Affinity Capture-MS Homo sapiens
42 SEC23IP 11196
Affinity Capture-MS Homo sapiens
43 MUC1 4582
Affinity Capture-MS Homo sapiens
44 CPSF7 79869
Affinity Capture-MS Homo sapiens
45 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 EWSR1 2130
Two-hybrid Homo sapiens
47 RPS9 6203
Affinity Capture-MS Homo sapiens
48 RPLP2 6181
Affinity Capture-MS Homo sapiens
49 KTN1 3895
Affinity Capture-MS Homo sapiens
50 LACTB 114294
Affinity Capture-MS Homo sapiens
51 DGCR2 9993
Affinity Capture-MS Homo sapiens
52 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
53 PTPN14 5784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ASH2L 9070
Two-hybrid Homo sapiens
55 POLR2C 5432
Affinity Capture-MS Homo sapiens
56 SNAP23 8773
Affinity Capture-MS Homo sapiens
57 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SMAD5 4090
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
59 H2AFY2 55506
Two-hybrid Homo sapiens
60 POLR2M  
Affinity Capture-MS Homo sapiens
61 WBP2 23558
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 STAT6 6778
Two-hybrid Homo sapiens
63 AMOT  
Affinity Capture-MS Homo sapiens
64 FAM120A 23196
Affinity Capture-MS Homo sapiens
65 DVL2 1856
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
66 RNF11 26994
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
67 MZT1  
Affinity Capture-MS Homo sapiens
68 GPN3  
Affinity Capture-MS Homo sapiens
69 TMEM255A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TRAF4 9618
Two-hybrid Homo sapiens
71 Bcas2  
Affinity Capture-MS Mus musculus
72 EDC4 23644
Affinity Capture-MS Homo sapiens
73 AMOTL2 51421
Affinity Capture-MS Homo sapiens
74 PHF20L1  
Two-hybrid Homo sapiens
75 HIST1H1T 3010
Affinity Capture-MS Homo sapiens
76 WRNIP1 56897
Affinity Capture-MS Homo sapiens
77 NPC1 4864
Affinity Capture-MS Homo sapiens
78 ARRDC1 92714
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
79 RAB14 51552
Affinity Capture-MS Homo sapiens
80 WBP1  
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 TMEM55B 90809
Affinity Capture-MS Homo sapiens
82 SRSF3 6428
Affinity Capture-MS Homo sapiens
83 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
84 CBL 867
Biochemical Activity Homo sapiens
85 PDLIM2  
Affinity Capture-MS Homo sapiens
86 DAZAP2  
Two-hybrid Homo sapiens
87 LAPTM5 7805
Two-hybrid Homo sapiens
88 RPL18A 6142
Affinity Capture-MS Homo sapiens
89 PTPN21 11099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 CCDC85C  
Affinity Capture-MS Homo sapiens
91 ARRDC2  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 SARAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 HIVEP3 59269
Affinity Capture-Western Homo sapiens
94 SMAD3 4088
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
95 SMAD2 4087
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
96 YWHAQ 10971
Reconstituted Complex Homo sapiens
97 SRSF1 6426
Affinity Capture-MS Homo sapiens
98 BCL2L1 598
Affinity Capture-MS Homo sapiens
99 UBE2D3 7323
Reconstituted Complex Homo sapiens
100 BRAF  
Affinity Capture-Western Homo sapiens
101 SRSF5 6430
Affinity Capture-MS Homo sapiens
102 LZTR1 8216
Two-hybrid Homo sapiens
103 RPS16 6217
Affinity Capture-MS Homo sapiens
104 ITCH 83737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 POLR2B 5431
Affinity Capture-MS Homo sapiens
106 MCTP2  
Affinity Capture-MS Homo sapiens
107 POLR2J  
Affinity Capture-MS Homo sapiens
108 PRRG4  
Reconstituted Complex Homo sapiens
109 RBBP6 5930
Affinity Capture-MS Homo sapiens
110 CASP14 23581
Affinity Capture-MS Homo sapiens
111 FNDC3B 64778
Affinity Capture-MS Homo sapiens
112 SLC2A1 6513
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
113 CBFA2T3  
Affinity Capture-MS Homo sapiens
114 ARRDC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 NDFIP2 54602
Affinity Capture-MS Homo sapiens
116 PNN 5411
Affinity Capture-MS Homo sapiens
117 ERBB2IP 55914
Affinity Capture-MS Homo sapiens
118 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
119 TMEM255B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 PRKAA2 5563
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
121 Smad3  
Affinity Capture-MS Mus musculus
122 MZT2B 80097
Affinity Capture-MS Homo sapiens
123 TRAF6 7189
Affinity Capture-Western Homo sapiens
124 PTEN 5728
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
125 ERBB4 2066
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
126 GPN1  
Affinity Capture-MS Homo sapiens
127 RPL3 6122
Affinity Capture-MS Homo sapiens
128 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
129 SETD4 54093
Affinity Capture-MS Homo sapiens
130 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
131 SERINC5 256987
Affinity Capture-MS Homo sapiens
132 TP73  
Affinity Capture-MS Homo sapiens
133 SMAD1 4086
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
134 PHB2 11331
Affinity Capture-MS Homo sapiens
135 UBE2D2 7322
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
136 POLR2D  
Affinity Capture-MS Homo sapiens
137 TJP2 9414
Affinity Capture-MS Homo sapiens
138 LDLRAD3  
Affinity Capture-MS Homo sapiens
139 ARRDC3 57561
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 RUNX2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
141 EEF1A2 1917
Affinity Capture-MS Homo sapiens
142 EEF1B2 1933
Affinity Capture-MS Homo sapiens
143 DDB1 1642
Affinity Capture-MS Homo sapiens
144 RRBP1 6238
Affinity Capture-MS Homo sapiens
145 SMAD6  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
146 PDE1C 5137
Affinity Capture-MS Homo sapiens
147 LATS1  
Protein-peptide Homo sapiens
148 POLR2A 5430
Affinity Capture-MS Homo sapiens
149 FLOT1 10211
Affinity Capture-MS Homo sapiens
150 XRCC5 7520
Affinity Capture-MS Homo sapiens
151 ICA1  
Affinity Capture-MS Homo sapiens
152 UBE2D1 7321
Reconstituted Complex Homo sapiens
153 RPL19 6143
Affinity Capture-MS Homo sapiens
154 RBM14 10432
Affinity Capture-MS Homo sapiens
155 FBXL15  
Affinity Capture-Western Homo sapiens
156 PDLIM7 9260
Affinity Capture-MS Homo sapiens
157 TMEM51 55092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 DVL1 1855
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
159 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
160 ESR2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
161 FAM175B 23172
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
162 DIDO1  
Two-hybrid Homo sapiens
163 SKIL  
Affinity Capture-Western Homo sapiens
164 SMAD7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
165 CPSF6 11052
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
166 TP53 7157
Reconstituted Complex Homo sapiens
167 CDK8 1024
Affinity Capture-MS Homo sapiens
168 U2AF1L4 199746
Affinity Capture-MS Homo sapiens
169 TUBG2  
Affinity Capture-MS Homo sapiens
170 ERH 2079
Affinity Capture-MS Homo sapiens
171 PDIA4 9601
Affinity Capture-MS Homo sapiens
172 EZR 7430
Affinity Capture-Western Homo sapiens
173 ARF5 381
Affinity Capture-MS Homo sapiens
174 PRRG1 5638
Affinity Capture-MS Homo sapiens
175 IKBKG 8517
Two-hybrid Homo sapiens
176 LITAF 9516
Affinity Capture-Western Homo sapiens
177 RPAP2  
Affinity Capture-MS Homo sapiens
178 THRAP3 9967
Affinity Capture-MS Homo sapiens
179 ILDR1 286676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 HSPB1 3315
Two-hybrid Homo sapiens
181 CLUH 23277
Affinity Capture-MS Homo sapiens
182 LRP12 29967
Affinity Capture-MS Homo sapiens
183 CAPNS1 826
Affinity Capture-MS Homo sapiens
184 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
185 EIF4A3 9775
Affinity Capture-MS Homo sapiens
186 SIRT1  
Affinity Capture-Western Homo sapiens
187 ARF4 378
Affinity Capture-MS Homo sapiens
188 NDFIP1 80762
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
189 SFTPC  
Reconstituted Complex Homo sapiens
190 KLF5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
191 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
192 TXN 7295
Affinity Capture-MS Homo sapiens
193 EZH2  
Affinity Capture-MS Homo sapiens
194 RPSA 3921
Affinity Capture-MS Homo sapiens
195 EEF1D 1936
Affinity Capture-MS Homo sapiens
196 HIST1H4J 8363
Affinity Capture-MS Homo sapiens
197 RPS7 6201
Affinity Capture-MS Homo sapiens
198 FBXL18 80028
Two-hybrid Homo sapiens
199 SPG20 23111
Affinity Capture-MS Homo sapiens