Gene description for WWP1
Gene name WW domain containing E3 ubiquitin protein ligase 1
Gene symbol WWP1
Other names/aliases AIP5
Tiul1
hSDRP1
Species Homo sapiens
 Database cross references - WWP1
ExoCarta ExoCarta_11059
Vesiclepedia VP_11059
Entrez Gene 11059
HGNC 17004
MIM 602307
UniProt Q9H0M0  
 WWP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for WWP1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 TAS
    ligase activity GO:0016874 IEA
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    protein ubiquitination GO:0016567 TAS
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    signal transduction GO:0007165 NAS
    transmembrane transport GO:0055085 TAS
    viral entry into host cell GO:0046718 TAS
    central nervous system development GO:0007417 NAS
    ion transmembrane transport GO:0034220 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IEA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
    ubiquitin ligase complex GO:0000151 NAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified WWP1 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WWP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DIDO1  
Two-hybrid Homo sapiens
2 DAZAP2  
Two-hybrid Homo sapiens
3 KLF2 10365
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 WBP2 23558
Protein-peptide Homo sapiens
5 EWSR1 2130
Two-hybrid Homo sapiens
6 SMAD5 4090
Affinity Capture-MS Homo sapiens
7 WBP1  
Protein-peptide Homo sapiens
8 CPSF6 11052
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 RNF11 26994
Affinity Capture-MS Homo sapiens
10 ATN1  
Two-hybrid Homo sapiens
11 ZNF638 27332
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here