Gene description for BRCC3
Gene name BRCA1/BRCA2-containing complex, subunit 3
Gene symbol BRCC3
Other names/aliases BRCC36
C6.1A
CXorf53
Species Homo sapiens
 Database cross references - BRCC3
ExoCarta ExoCarta_79184
Vesiclepedia VP_79184
Entrez Gene 79184
HGNC 24185
MIM 300617
UniProt P46736  
 BRCC3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for BRCC3
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IEA
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 IBA
    metallopeptidase activity GO:0008237 IMP
    enzyme regulator activity GO:0030234 IDA
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
    metal ion binding GO:0046872 IEA
    K63-linked deubiquitinase activity GO:0061578 IDA
    K63-linked deubiquitinase activity GO:0061578 TAS
    metal-dependent deubiquitinase activity GO:0140492 IMP
Biological Process
    regulation of DNA repair GO:0006282 NAS
    double-strand break repair GO:0006302 IBA
    double-strand break repair GO:0006302 IMP
    proteolysis GO:0006508 IEA
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IMP
    response to X-ray GO:0010165 IDA
    response to ionizing radiation GO:0010212 IMP
    protein deubiquitination GO:0016579 TAS
    mitotic G2/M transition checkpoint GO:0044818 NAS
    positive regulation of DNA repair GO:0045739 IMP
    cell division GO:0051301 IEA
    protein K63-linked deubiquitination GO:0070536 IDA
    protein K63-linked deubiquitination GO:0070536 IMP
    cellular response to ionizing radiation GO:0071479 IMP
    DNA repair-dependent chromatin remodeling GO:0140861 IMP
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 IDA
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 ISS
    regulation of DNA damage checkpoint GO:2000001 NAS
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IDA
    nuclear ubiquitin ligase complex GO:0000152 IDA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    BRCA1-A complex GO:0070531 IBA
    BRCA1-A complex GO:0070531 IDA
    BRCA1-A complex GO:0070531 NAS
    BRISC complex GO:0070552 IBA
    BRISC complex GO:0070552 IDA
 Experiment description of studies that identified BRCC3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BRCC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 PAXIP1  
Affinity Capture-MS Homo sapiens
4 POT1  
Co-fractionation Homo sapiens
5 MSH6 2956
Co-fractionation Homo sapiens
6 WWP1 11059
Affinity Capture-MS Homo sapiens
7 RABEP2 79874
Co-fractionation Homo sapiens
8 BRCA1 672
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
9 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
10 DNAJC25 548645
Affinity Capture-MS Homo sapiens
11 CAND1 55832
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
12 HNRNPR 10236
Co-fractionation Homo sapiens
13 CENPA  
Affinity Capture-MS Homo sapiens
14 C2orf73  
Affinity Capture-MS Homo sapiens
15 TRIM28 10155
Co-fractionation Homo sapiens
16 RNF20 56254
Co-fractionation Homo sapiens
17 C9orf85  
Affinity Capture-MS Homo sapiens
18 MCM2 4171
Affinity Capture-MS Homo sapiens
19 GPHN 10243
Co-fractionation Homo sapiens
20 SPAG9 9043
Co-fractionation Homo sapiens
21 COPS5 10987
Affinity Capture-Western Homo sapiens
22 BRCC3 79184
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 MDC1  
Affinity Capture-MS Homo sapiens
24 DDOST 1650
Affinity Capture-MS Homo sapiens
25 CCDC6 8030
Co-fractionation Homo sapiens
26 ATP5O 539
Affinity Capture-MS Homo sapiens
27 FAF1 11124
Co-fractionation Homo sapiens
28 Nedd1  
Affinity Capture-MS Mus musculus
29 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 FBXO7 25793
Co-fractionation Homo sapiens
31 SNX27 81609
Affinity Capture-MS Homo sapiens
32 USP7 7874
Affinity Capture-MS Homo sapiens
33 MTA2 9219
Co-fractionation Homo sapiens
34 UIMC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
35 AURKA 6790
Affinity Capture-Western Homo sapiens
36 DNAL4  
Affinity Capture-MS Homo sapiens
37 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
38 BRE 9577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
39 SMEK1 55671
Co-fractionation Homo sapiens
40 RAD51  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
41 BRCA2 675
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 FANCG 2189
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
43 DRAP1 10589
Co-fractionation Homo sapiens
44 BARD1 580
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 RNF166  
Affinity Capture-MS Homo sapiens
46 SUMO2 6613
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 Tnks2  
Affinity Capture-MS Mus musculus
48 BRAP  
Co-fractionation Homo sapiens
49 CD2AP 23607
Co-fractionation Homo sapiens
50 NUMA1 4926
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
51 RNF40 9810
Co-fractionation Homo sapiens
52 SNAPC5  
Two-hybrid Homo sapiens
53 PPP4R2  
Co-fractionation Homo sapiens
54 SMARCD1 6602
Affinity Capture-MS Homo sapiens
55 E2F5  
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
57 NR3C1 2908
Co-fractionation Homo sapiens
58 UBE2E1  
Co-fractionation Homo sapiens
59 TFPT  
Affinity Capture-MS Homo sapiens
60 SF3A1 10291
Co-fractionation Homo sapiens
61 MRPL1  
Affinity Capture-MS Homo sapiens
62 FANCA  
Affinity Capture-Luminescence Homo sapiens
63 RPA3 6119
Proximity Label-MS Homo sapiens
64 NLRP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 JUNB  
Affinity Capture-MS Homo sapiens
66 BAZ1A 11177
Co-fractionation Homo sapiens
67 NDUFA10 4705
Affinity Capture-MS Homo sapiens
68 Rpl35 66489
Affinity Capture-MS Mus musculus
69 SUPT16H 11198
Affinity Capture-MS Homo sapiens
70 HUWE1 10075
Co-fractionation Homo sapiens
71 UBE2D1 7321
Reconstituted Complex Homo sapiens
72 STUB1 10273
Co-fractionation Homo sapiens
73 FAM175B 23172
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
74 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 TRAF2 7186
Co-fractionation Homo sapiens
76 GRB2 2885
Affinity Capture-MS Homo sapiens
77 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
78 EXOC8 149371
Affinity Capture-MS Homo sapiens
79 UBL4B  
Affinity Capture-MS Homo sapiens
80 TNS1 7145
Affinity Capture-MS Homo sapiens
81 SQSTM1 8878
Proximity Label-MS Homo sapiens
82 HDAC1 3065
Co-fractionation Homo sapiens
83 FAM175A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
84 SHMT2 6472
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
85 RABL6 55684
Co-fractionation Homo sapiens
86 BABAM1 29086
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
87 WWP2 11060
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
88 LARP7 51574
Co-fractionation Homo sapiens
89 ANKRD11 29123
Two-hybrid Homo sapiens
90 STK11 6794
Negative Genetic Homo sapiens
91 C9orf72  
Affinity Capture-MS Homo sapiens
92 NUCKS1 64710
Co-fractionation Homo sapiens
93 WDHD1  
Co-fractionation Homo sapiens
View the network image/svg+xml



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