Gene description for GPHN
Gene name gephyrin
Gene symbol GPHN
Other names/aliases GEPH
GPH
GPHRYN
HKPX1
MOCODC
Species Homo sapiens
 Database cross references - GPHN
ExoCarta ExoCarta_10243
Vesiclepedia VP_10243
Entrez Gene 10243
HGNC 15465
MIM 603930
UniProt Q9NQX3  
 GPHN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GPHN
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    nitrate reductase activity GO:0008940 IMP
    protein-macromolecule adaptor activity GO:0030674 IMP
    identical protein binding GO:0042802 IDA
    molybdopterin cofactor binding GO:0043546 IDA
    metal ion binding GO:0046872 IEA
    molybdopterin adenylyltransferase activity GO:0061598 IEA
    molybdopterin molybdotransferase activity GO:0061599 IBA
Biological Process
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IBA
    establishment of synaptic specificity at neuromuscular junction GO:0007529 IBA
    response to metal ion GO:0010038 IMP
    molybdopterin cofactor biosynthetic process GO:0032324 IDA
    molybdopterin cofactor biosynthetic process GO:0032324 IMP
    glycine receptor clustering GO:0072579 IBA
    gamma-aminobutyric acid receptor clustering GO:0097112 IBA
    gamma-aminobutyric acid receptor clustering GO:0097112 IDA
    gamma-aminobutyric acid receptor clustering GO:0097112 IMP
    postsynaptic neurotransmitter receptor diffusion trapping GO:0098970 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 TAS
    postsynaptic density GO:0014069 ISS
    dendrite GO:0030425 IBA
    dendrite GO:0030425 IDA
    dendritic spine GO:0043197 IDA
    postsynaptic membrane GO:0045211 IBA
    postsynaptic membrane GO:0045211 IDA
    postsynaptic membrane GO:0045211 IDA
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 ISS
    postsynaptic specialization GO:0099572 IBA
    postsynaptic specialization membrane GO:0099634 IEA
 Experiment description of studies that identified GPHN in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GPHN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 CDCA5  
Affinity Capture-MS Homo sapiens
3 POLR2C 5432
Proximity Label-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 HNRNPC 3183
Affinity Capture-MS Homo sapiens
6 SENP3 26168
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PPP4R1 9989
Co-fractionation Homo sapiens
9 ARHGAP32  
Affinity Capture-MS Homo sapiens
10 MTA2 9219
Co-fractionation Homo sapiens
11 TP53 7157
Affinity Capture-MS Homo sapiens
12 PRNP 5621
Affinity Capture-MS Homo sapiens
13 GPHN 10243
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 PFN1 5216
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
15 ACTC1 70
Proximity Label-MS Homo sapiens
16 ARHGEF9  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
17 CUL3 8452
Affinity Capture-MS Homo sapiens
18 ARSH  
Affinity Capture-MS Homo sapiens
19 ROCK2 9475
Co-fractionation Homo sapiens
20 PARK2  
Affinity Capture-MS Homo sapiens
21 ACADVL 37
Two-hybrid Homo sapiens
22 DVL1 1855
Affinity Capture-MS Homo sapiens
23 VASP 7408
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 ZAR1L  
Affinity Capture-MS Homo sapiens
25 PSMA1 5682
Two-hybrid Homo sapiens
26 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ODF3L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PAAF1 80227
Co-fractionation Homo sapiens
29 ACTG1 71
Affinity Capture-MS Homo sapiens
30 OTUD4  
Affinity Capture-MS Homo sapiens
31 TCF4  
Affinity Capture-MS Homo sapiens
32 SPTAN1 6709
Co-fractionation Homo sapiens
33 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SPATS1  
Two-hybrid Homo sapiens
35 NTRK1 4914
Affinity Capture-MS Homo sapiens
36 PCK1 5105
Affinity Capture-MS Homo sapiens
37 RAB7A 7879
Proximity Label-MS Homo sapiens
38 MTOR 2475
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 KLKB1 3818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 STS 412
Affinity Capture-MS Homo sapiens
41 KMO  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ZC2HC1C  
Affinity Capture-MS Homo sapiens
43 Mtor 56718
Two-hybrid Rattus norvegicus
44 CLTB 1212
Proximity Label-MS Homo sapiens
45 BRCC3 79184
Co-fractionation Homo sapiens
46 PRPF4 9128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 Dynll1 56455
Affinity Capture-MS Mus musculus
48 ANAPC4 29945
Affinity Capture-MS Homo sapiens
49 KHDRBS1 10657
Two-hybrid Homo sapiens
50 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 AKT1 207
Affinity Capture-MS Homo sapiens
52 GLRB  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
53 ENAH 55740
Affinity Capture-Western Homo sapiens
54 NAA15 80155
Affinity Capture-MS Homo sapiens
55 TSSK1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 EP300 2033
Affinity Capture-MS Homo sapiens
57 MITD1 129531
Two-hybrid Homo sapiens
View the network image/svg+xml



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