Gene description for GPHN
Gene name gephyrin
Gene symbol GPHN
Other names/aliases GEPH
GPH
GPHRYN
HKPX1
MOCODC
Species Homo sapiens
 Database cross references - GPHN
ExoCarta ExoCarta_10243
Vesiclepedia VP_10243
Entrez Gene 10243
HGNC 15465
MIM 603930
UniProt Q9NQX3  
 GPHN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GPHN
Molecular Function
    molybdopterin molybdotransferase activity GO:0061599 TAS
    ATP binding GO:0005524 IEA
    molybdopterin adenylyltransferase activity GO:0061598 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    glycine receptor clustering GO:0072579 IBA
    vitamin metabolic process GO:0006766 TAS
    water-soluble vitamin metabolic process GO:0006767 TAS
    establishment of synaptic specificity at neuromuscular junction GO:0007529 IEA
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IBA
    molybdopterin cofactor biosynthetic process GO:0032324 TAS
    small molecule metabolic process GO:0044281 TAS
    molybdenum incorporation into molybdenum-molybdopterin complex GO:0018315 IBA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cell junction GO:0030054 IEA
    cytoplasm GO:0005737 IBA
    cytoskeleton GO:0005856 IEA
    inhibitory synapse GO:0060077 IEA
    postsynaptic membrane GO:0045211 IEA
 Experiment description of studies that identified GPHN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GPHN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PFN1 5216
Invitro Homo sapiens
2 Mtor 56718
Two-hybrid Rattus norvegicus
3 GARS 2617
Invivo Homo sapiens
Invitro Homo sapiens
4 ARHGEF9  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 DYNLL2 140735
Invitro Homo sapiens
6 GLRB  
Reconstituted Complex Homo sapiens
7 DYNLL1 8655
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
8 MTOR 2475
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which GPHN is involved
PathwayEvidenceSource
Molybdenum cofactor biosynthesis TAS Reactome





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