Experiment description of studies that identified PAAF1 in exosomes

1 Experiment ID 497 MISEV standards ✘ EV Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 488 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for PAAF1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PSMD11 5717 Affinity Capture-MS Homo sapiens 2 PSMC1 5700 Affinity Capture-MS Homo sapiens 3 PSMC5 5705 Affinity Capture-MS Homo sapiens 4 PSMD14 10213 Affinity Capture-MS Homo sapiens 5 PSMD8 5714 Affinity Capture-MS Homo sapiens 6 PSMD12 5718 Affinity Capture-MS Homo sapiens 7 PSMC6 5706 Affinity Capture-MS Homo sapiens 8 PSMD1 5707 Affinity Capture-MS Homo sapiens 9 ALDH16A1 126133 Affinity Capture-MS Homo sapiens 10 DERA 51071 Affinity Capture-MS Homo sapiens 11 PSMC4 5704 Affinity Capture-MS Homo sapiens 12 PSMC2 5701 Affinity Capture-MS Homo sapiens 13 PSMD2 5708 Affinity Capture-MS Homo sapiens 14 PSMC3 5702 Affinity Capture-MS Homo sapiens 15 PSMD6 9861 Affinity Capture-MS Homo sapiens 16 PSMD10 5716 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 PSMD3 5709 Affinity Capture-MS Homo sapiens 18 PSMD7 5713 Affinity Capture-MS Homo sapiens 19 FADS1 3992 Affinity Capture-MS Homo sapiens View the network

Pathways in which PAAF1 is involved

No pathways found