Gene description for FADS1
Gene name fatty acid desaturase 1
Gene symbol FADS1
Other names/aliases D5D
FADS6
FADSD5
LLCDL1
TU12
Species Homo sapiens
 Database cross references - FADS1
ExoCarta ExoCarta_3992
Vesiclepedia VP_3992
Entrez Gene 3992
HGNC 3574
MIM 606148
UniProt O60427  
 FADS1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FADS1
Molecular Function
    C-5 sterol desaturase activity GO:0000248 TAS
    linoleoyl-CoA desaturase activity GO:0016213 IDA
    oxidoreductase activity GO:0016491 IDA
    oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water GO:0016717 IBA
    omega-6 fatty acid desaturase activity GO:0045485 TAS
    acyl-CoA delta5-desaturase activity GO:0062076 IDA
Biological Process
    regulation of DNA-templated transcription GO:0006355 NAS
    lipid metabolic process GO:0006629 IBA
    unsaturated fatty acid biosynthetic process GO:0006636 IDA
    unsaturated fatty acid biosynthetic process GO:0006636 IDA
    unsaturated fatty acid biosynthetic process GO:0006636 IEA
    cell-cell signaling GO:0007267 NAS
    phospholipid biosynthetic process GO:0008654 TAS
    cellular response to starvation GO:0009267 IDA
    alpha-linolenic acid metabolic process GO:0036109 TAS
    long-chain fatty acid biosynthetic process GO:0042759 IDA
    linoleic acid metabolic process GO:0043651 TAS
    regulation of cell differentiation GO:0045595 NAS
    icosanoid biosynthetic process GO:0046456 TAS
Subcellular Localization
    mitochondrion GO:0005739 ISS
    endoplasmic reticulum membrane GO:0005789 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 NAS
    intracellular membrane-bounded organelle GO:0043231 NAS
 Experiment description of studies that identified FADS1 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FADS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OR2M7  
Affinity Capture-MS Homo sapiens
2 GRPR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GPR17 2840
Affinity Capture-MS Homo sapiens
5 EIF3G 8666
Affinity Capture-MS Homo sapiens
6 TMEM63C  
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 NKAIN1  
Affinity Capture-MS Homo sapiens
9 SV2A 9900
Affinity Capture-MS Homo sapiens
10 ATP13A3 79572
Affinity Capture-MS Homo sapiens
11 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PTH1R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CXCR4 7852
Affinity Capture-MS Homo sapiens
15 FAM20C 56975
Affinity Capture-MS Homo sapiens
16 GABRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RNF181  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FPR1  
Affinity Capture-MS Homo sapiens
19 UQCC1 55245
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZDHHC12  
Affinity Capture-MS Homo sapiens
21 DHCR7 1717
Affinity Capture-MS Homo sapiens
22 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
23 GPR182  
Affinity Capture-MS Homo sapiens
24 BSCL2  
Affinity Capture-MS Homo sapiens
25 HCRTR2  
Affinity Capture-MS Homo sapiens
26 TSPAN15 23555
Affinity Capture-MS Homo sapiens
27 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
28 GCGR  
Affinity Capture-MS Homo sapiens
29 VSTM1  
Affinity Capture-MS Homo sapiens
30 SLC5A6 8884
Affinity Capture-MS Homo sapiens
31 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 OPRL1 4987
Affinity Capture-MS Homo sapiens
33 SLC7A8 23428
Affinity Capture-MS Homo sapiens
34 XRN1 54464
Two-hybrid Homo sapiens
35 MS4A15  
Affinity Capture-MS Homo sapiens
36 CD80 941
Affinity Capture-MS Homo sapiens
37 PAAF1 80227
Affinity Capture-MS Homo sapiens
38 B3GALT5  
Affinity Capture-MS Homo sapiens
39 PLD3 23646
Affinity Capture-MS Homo sapiens
40 PARP1 142
Proximity Label-MS Homo sapiens
41 SLC5A4  
Affinity Capture-MS Homo sapiens
42 ABCA6 23460
Affinity Capture-MS Homo sapiens
43 BACE2 25825
Affinity Capture-MS Homo sapiens
44 SLC22A2 6582
Affinity Capture-MS Homo sapiens
45 NPLOC4 55666
Affinity Capture-MS Homo sapiens
46 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 HARS2 23438
Affinity Capture-MS Homo sapiens
48 SLC24A3  
Affinity Capture-MS Homo sapiens
49 PTCHD3  
Affinity Capture-MS Homo sapiens
50 SIRT2 22933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 KCNA2  
Affinity Capture-MS Homo sapiens
52 CCDC22 28952
Affinity Capture-MS Homo sapiens
53 IL17RC 84818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CD63 967
Affinity Capture-MS Homo sapiens
55 SLC22A4 6583
Affinity Capture-MS Homo sapiens
56 CMKLR1 1240
Affinity Capture-MS Homo sapiens
57 TMCO3 55002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 TTYH1  
Affinity Capture-MS Homo sapiens
59 AVPR2  
Affinity Capture-MS Homo sapiens
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