Gene description for CCDC22
Gene name coiled-coil domain containing 22
Gene symbol CCDC22
Other names/aliases CXorf37
JM1
Species Homo sapiens
 Database cross references - CCDC22
ExoCarta ExoCarta_28952
Vesiclepedia VP_28952
Entrez Gene 28952
HGNC 28909
MIM 300859
UniProt O60826  
 CCDC22 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for CCDC22
Molecular Function
    protein binding GO:0005515 IPI
    cullin family protein binding GO:0097602 IBA
    cullin family protein binding GO:0097602 IDA
Biological Process
    intracellular copper ion homeostasis GO:0006878 IMP
    Golgi to plasma membrane transport GO:0006893 IMP
    cytoplasmic sequestering of NF-kappaB GO:0007253 IMP
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IMP
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IBA
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    endosome GO:0005768 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CCDC22 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CCDC22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC151  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ARHGAP1 392
Co-fractionation Homo sapiens
3 BLOC1S6  
Two-hybrid Homo sapiens
4 UBE2M 9040
Affinity Capture-MS Homo sapiens
5 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SYCE1  
Affinity Capture-MS Homo sapiens
7 PFKP 5214
Co-fractionation Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 NUP62 23636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COMMD9 29099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CDC26 246184
Affinity Capture-MS Homo sapiens
12 FAM32A  
Affinity Capture-MS Homo sapiens
13 CAMSAP2  
Proximity Label-MS Homo sapiens
14 STX4 6810
Proximity Label-MS Homo sapiens
15 DECR1 1666
Affinity Capture-MS Homo sapiens
16 VPS29 51699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CAPZB 832
Affinity Capture-MS Homo sapiens
18 C16orf62 57020
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
19 NDE1 54820
Affinity Capture-MS Homo sapiens
20 SULF2 55959
Affinity Capture-MS Homo sapiens
21 NDRG1 10397
Co-fractionation Homo sapiens
22 FAM45A 404636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DSCR3 10311
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CTAGE5 4253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 LRRC48  
Affinity Capture-MS Homo sapiens
26 MTMR2 8898
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ABI1 10006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 XPO7 23039
Co-fractionation Homo sapiens
29 PSMG1 8624
Co-fractionation Homo sapiens
30 COMMD10 51397
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TRIM16L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ZWINT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 OIP5  
Two-hybrid Homo sapiens
34 CUL1 8454
Co-fractionation Homo sapiens
35 KNSTRN  
Affinity Capture-MS Homo sapiens
36 OR13G1  
Affinity Capture-MS Homo sapiens
37 COMMD4 54939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 VIPAS39 63894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EXOC3 11336
Affinity Capture-MS Homo sapiens
40 CCDC114  
Affinity Capture-MS Homo sapiens
41 C15orf59  
Affinity Capture-MS Homo sapiens
42 KRT8 3856
Proximity Label-MS Homo sapiens
43 COMMD7 149951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SRGAP3  
Affinity Capture-MS Homo sapiens
45 HPS5 11234
Affinity Capture-MS Homo sapiens
46 GMDS 2762
Affinity Capture-MS Homo sapiens
47 IKBIP 121457
Cross-Linking-MS (XL-MS) Homo sapiens
48 USP14 9097
Co-fractionation Homo sapiens
49 FADS1 3992
Affinity Capture-MS Homo sapiens
50 NONO 4841
Affinity Capture-MS Homo sapiens
51 PSMG2 56984
Co-fractionation Homo sapiens
52 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TRIM16 10626
Affinity Capture-MS Homo sapiens
54 CCDC107  
Affinity Capture-MS Homo sapiens
55 PEX14 5195
Proximity Label-MS Homo sapiens
56 ACTB 60
Proximity Label-MS Homo sapiens
57 PLXNA3 55558
Affinity Capture-MS Homo sapiens
58 CUL2 8453
Co-fractionation Homo sapiens
59 TRIM72  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 COMMD3 23412
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 ACTG1 71
Two-hybrid Homo sapiens
62 DISC1 27185
Affinity Capture-MS Homo sapiens
63 CNGA4  
Affinity Capture-MS Homo sapiens
64 WHAMMP3  
Affinity Capture-MS Homo sapiens
65 Ranbp1 19385
Affinity Capture-MS Mus musculus
66 MAP7 9053
Affinity Capture-MS Homo sapiens
67 CUL3 8452
Co-fractionation Homo sapiens
68 STMN2  
Affinity Capture-MS Homo sapiens
69 POTEI 653269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 COMMD8 54951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
71 IFT57 55081
Affinity Capture-MS Homo sapiens
72 COMMD2 51122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 RPRD1A  
Co-fractionation Homo sapiens
74 PACSIN3 29763
Affinity Capture-MS Homo sapiens
75 CNKSR3 154043
Affinity Capture-MS Homo sapiens
76 PSMD7 5713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 C14orf166 51637
Co-fractionation Homo sapiens
78 KRT27 342574
Two-hybrid Homo sapiens
79 BIRC6 57448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 PSPC1 55269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 RPA3 6119
Proximity Label-MS Homo sapiens
83 NIN 51199
Proximity Label-MS Homo sapiens
84 COMMD6 170622
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 FAM21C 253725
Affinity Capture-Western Homo sapiens
86 NDC80 10403
Proximity Label-MS Homo sapiens
87 SORBS3 10174
Affinity Capture-MS Homo sapiens
88 BAZ1A 11177
Affinity Capture-MS Homo sapiens
89 USHBP1  
Two-hybrid Homo sapiens
90 PCM1 5108
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CYFIP2 26999
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 KRT19 3880
Proximity Label-MS Homo sapiens
93 ARAF 369
Affinity Capture-MS Homo sapiens
94 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 NEDD4L 23327
Affinity Capture-MS Homo sapiens
96 OAS3 4940
Affinity Capture-MS Homo sapiens
97 COMMD1 150684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
98 PFAS 5198
Co-fractionation Homo sapiens
99 KRT18 3875
Proximity Label-MS Homo sapiens
100 MFHAS1 9258
Affinity Capture-MS Homo sapiens
101 TOE1 114034
Affinity Capture-MS Homo sapiens
102 ARHGEF33  
Affinity Capture-MS Homo sapiens
103 COMMD5 28991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 SAP18 10284
Affinity Capture-MS Homo sapiens
105 SEPT10 151011
Proximity Label-MS Homo sapiens
106 DCTN2 10540
Proximity Label-MS Homo sapiens
107 CCDC93 54520
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
108 TRIM25 7706
Co-fractionation Homo sapiens
109 COMMD3-BMI1 100532731
Affinity Capture-MS Homo sapiens
110 NUP50 10762
Co-fractionation Homo sapiens
111 ABI2 10152
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 KRT38 8687
Affinity Capture-MS Homo sapiens
113 SPAG5 10615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 RTCB 51493
Co-fractionation Homo sapiens
115 SH3GLB1 51100
Co-fractionation Homo sapiens
116 CEP135  
Proximity Label-MS Homo sapiens
117 PRDX1 5052
Affinity Capture-MS Homo sapiens
118 CNGA3  
Affinity Capture-MS Homo sapiens
119 ZUFSP 221302
Affinity Capture-MS Homo sapiens
120 UHRF1 29128
Affinity Capture-MS Homo sapiens
121 HSPG2 3339
Affinity Capture-MS Homo sapiens
122 CCDC96  
Affinity Capture-MS Homo sapiens
123 WARS 7453
Co-fractionation Homo sapiens
124 VPS33B 26276
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 C17orf59 54785
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CCDC22 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Neddylation TAS Reactome
Post-translational protein modification TAS Reactome





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