Gene description for C14orf166
Gene name chromosome 14 open reading frame 166
Gene symbol C14orf166
Other names/aliases CGI-99
CGI99
CLE
CLE7
LCRP369
RLLM1
Species Homo sapiens
 Database cross references - C14orf166
ExoCarta ExoCarta_51637
Entrez Gene 51637
HGNC 23169
MIM 610858
UniProt Q9Y224  
 C14orf166 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for C14orf166
Molecular Function
    identical protein binding GO:0042802 IPI
    RNA polymerase II core binding GO:0000993 IDA
    poly(A) RNA binding GO:0044822 IDA
    RNA binding GO:0003723 IDA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    RNA transport GO:0050658 TAS
    transcription, DNA-templated GO:0006351 IEA
    viral process GO:0016032 IEA
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    microtubule organizing center GO:0005815 IEA
    cytosol GO:0005829 IEA
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    tRNA-splicing ligase complex GO:0072669 IDA
 Experiment description of studies that identified C14orf166 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C14orf166
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C14orf166 51637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 VCL 7414
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which C14orf166 is involved
No pathways found





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