Gene description for EIF2B2
Gene name eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Gene symbol EIF2B2
Other names/aliases EIF-2Bbeta
EIF2B
Species Homo sapiens
 Database cross references - EIF2B2
ExoCarta ExoCarta_8892
Vesiclepedia VP_8892
Entrez Gene 8892
HGNC 3258
MIM 606454
UniProt P49770  
 EIF2B2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for EIF2B2
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    GTP binding GO:0005525 IDA
Biological Process
    ovarian follicle development GO:0001541 IMP
    cytoplasmic translational initiation GO:0002183 IDA
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IDA
    regulation of translational initiation GO:0006446 TAS
    central nervous system development GO:0007417 IMP
    response to heat GO:0009408 ISS
    response to heat GO:0009408 TAS
    response to glucose GO:0009749 ISS
    oligodendrocyte development GO:0014003 IMP
    myelination GO:0042552 IMP
    response to peptide hormone GO:0043434 ISS
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 2B complex GO:0005851 IBA
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
 Experiment description of studies that identified EIF2B2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM164  
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UTP14A 10813
Affinity Capture-MS Homo sapiens
4 CCT3 7203
Two-hybrid Homo sapiens
5 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 EIF2S3 1968
Affinity Capture-MS Homo sapiens
7 EIF3K 27335
Co-fractionation Homo sapiens
8 WNK1 65125
Co-fractionation Homo sapiens
9 BZW2 28969
Affinity Capture-MS Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 ABCF1 23
Co-fractionation Homo sapiens
12 TMEM206 55248
Affinity Capture-MS Homo sapiens
13 RPL10 6134
Affinity Capture-MS Homo sapiens
14 NUMA1 4926
Affinity Capture-MS Homo sapiens
15 PNKD  
Affinity Capture-MS Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 ILF2 3608
Co-fractionation Homo sapiens
18 DIMT1 27292
Co-fractionation Homo sapiens
19 MRFAP1L1  
Two-hybrid Homo sapiens
20 IGF2BP3 10643
Co-fractionation Homo sapiens
21 MCU 90550
Affinity Capture-MS Homo sapiens
22 IGSF8 93185
Affinity Capture-MS Homo sapiens
23 FAM174A 345757
Affinity Capture-MS Homo sapiens
24 CLEC2B  
Affinity Capture-MS Homo sapiens
25 SLC27A2 11001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 DDRGK1 65992
Affinity Capture-MS Homo sapiens
27 QARS 5859
Co-fractionation Homo sapiens
28 SYT2  
Affinity Capture-MS Homo sapiens
29 SLC10A7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ENPP1 5167
Affinity Capture-MS Homo sapiens
32 TACSTD2 4070
Affinity Capture-MS Homo sapiens
33 CDC27 996
Co-fractionation Homo sapiens
34 ATG16L1 55054
Affinity Capture-MS Homo sapiens
35 SELT 51714
Affinity Capture-MS Homo sapiens
36 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NMT1 4836
Co-fractionation Homo sapiens
38 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 GPR17 2840
Affinity Capture-MS Homo sapiens
41 EPB41L3 23136
Affinity Capture-MS Homo sapiens
42 CD70 970
Affinity Capture-MS Homo sapiens
43 DLD 1738
Affinity Capture-MS Homo sapiens
44 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CMAS 55907
Affinity Capture-MS Homo sapiens
46 MRE11A 4361
Co-fractionation Homo sapiens
47 EXOG 9941
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TMEM55B 90809
Affinity Capture-MS Homo sapiens
49 EIF2B5 8893
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
50 COMTD1 118881
Affinity Capture-MS Homo sapiens
51 Srp72  
Affinity Capture-MS Mus musculus
52 B3GALNT2 148789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RFC4 5984
Co-fractionation Homo sapiens
54 DDX1 1653
Co-fractionation Homo sapiens
55 DHX9 1660
Co-fractionation Homo sapiens
56 EIF2B4 8890
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
57 ZNF326 284695
Affinity Capture-MS Homo sapiens
58 P2RX4 5025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 CD40 958
Affinity Capture-MS Homo sapiens
60 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 FGF14  
Affinity Capture-MS Homo sapiens
63 BTNL9  
Affinity Capture-MS Homo sapiens
64 XPO1 7514
Affinity Capture-MS Homo sapiens
65 CASC4 113201
Affinity Capture-MS Homo sapiens
66 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 EIF2B3 8891
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
68 SPTBN1 6711
Co-fractionation Homo sapiens
69 CD226 10666
Affinity Capture-MS Homo sapiens
70 SMDT1  
Affinity Capture-MS Homo sapiens
71 MFAP1  
Co-fractionation Homo sapiens
72 C3orf18  
Affinity Capture-MS Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 CSNK2A2 1459
Two-hybrid Homo sapiens
75 DCC  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FKBP3 2287
Co-fractionation Homo sapiens
77 PVRL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 ENTPD2 954
Affinity Capture-MS Homo sapiens
79 EIF2B1 1967
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
80 TMEM55A 55529
Affinity Capture-MS Homo sapiens
81 NELFB 25920
Co-fractionation Homo sapiens
82 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 C9orf72  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
84 PTAFR  
Affinity Capture-MS Homo sapiens
85 YBX1 4904
Co-fractionation Homo sapiens
86 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
87 PARP1 142
Proximity Label-MS Homo sapiens
88 PCDH9  
Affinity Capture-MS Homo sapiens
89 BTC  
Affinity Capture-MS Homo sapiens
90 HNRNPA2B1 3181
Co-fractionation Homo sapiens
91 HNRNPM 4670
Co-fractionation Homo sapiens
92 PTBP1 5725
Co-fractionation Homo sapiens
93 EFNB1 1947
Affinity Capture-MS Homo sapiens
94 OPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 EDC4 23644
Co-fractionation Homo sapiens
98 OGFR 11054
Co-fractionation Homo sapiens
99 SPN 6693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 SEC62 7095
Affinity Capture-MS Homo sapiens
101 RTCB 51493
Co-fractionation Homo sapiens
102 YBX3 8531
Co-fractionation Homo sapiens
103 PCGF6  
Two-hybrid Homo sapiens
104 ATP2B2 491
Affinity Capture-MS Homo sapiens
105 C14orf166 51637
Co-fractionation Homo sapiens
106 TPST2 8459
Affinity Capture-MS Homo sapiens
107 KRAS 3845
Negative Genetic Homo sapiens
108 HNRNPU 3192
Co-fractionation Homo sapiens
109 GNB2L1 10399
Affinity Capture-MS Homo sapiens
110 GOLM1 51280
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 RC3H1 149041
Affinity Capture-MS Homo sapiens
112 PTRF 284119
Co-fractionation Homo sapiens
113 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 EIF5 1983
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
115 UBA52 7311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 KIAA1919  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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