Gene description for WNK1
Gene name WNK lysine deficient protein kinase 1
Gene symbol WNK1
Other names/aliases HSAN2
HSN2
KDP
PPP1R167
PRKWNK1
PSK
p65
Species Homo sapiens
 Database cross references - WNK1
ExoCarta ExoCarta_65125
Vesiclepedia VP_65125
Entrez Gene 65125
HGNC 14540
MIM 605232
UniProt Q9H4A3  
 WNK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for WNK1
Molecular Function
    protein kinase activity GO:0004672 IMP
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISS
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein kinase binding GO:0019901 IPI
    phosphatase binding GO:0019902 IDA
    protein kinase activator activity GO:0030295 IMP
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    molecular condensate scaffold activity GO:0140693 IDA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    regulation of sodium ion transport GO:0002028 ISS
    positive regulation of systemic arterial blood pressure GO:0003084 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    monoatomic ion transport GO:0006811 ISS
    cell volume homeostasis GO:0006884 IDA
    DNA damage response GO:0006974 IBA
    signal transduction GO:0007165 IDA
    heart development GO:0007507 IEA
    negative regulation of autophagy GO:0010507 IMP
    negative regulation of sodium ion transport GO:0010766 ISS
    regulation of mRNA export from nucleus GO:0010793 IDA
    positive regulation of T cell chemotaxis GO:0010820 IMP
    peptidyl-serine phosphorylation GO:0018105 ISS
    peptidyl-threonine phosphorylation GO:0018107 IMP
    peptidyl-threonine phosphorylation GO:0018107 ISS
    peptidyl-threonine phosphorylation GO:0018107 TAS
    intracellular chloride ion homeostasis GO:0030644 IEA
    negative regulation of protein ubiquitination GO:0031397 IDA
    negative regulation of cell-cell adhesion mediated by integrin GO:0033633 IMP
    negative regulation of heterotypic cell-cell adhesion GO:0034115 IMP
    negative regulation of GTPase activity GO:0034260 IMP
    intracellular signal transduction GO:0035556 IDA
    intracellular signal transduction GO:0035556 TAS
    sodium ion transmembrane transport GO:0035725 IEA
    chemokine (C-C motif) ligand 21 signaling pathway GO:0038116 ISS
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    neuron development GO:0048666 NAS
    T cell receptor signaling pathway GO:0050852 ISS
    potassium ion homeostasis GO:0055075 IEA
    monoatomic cation homeostasis GO:0055080 ISS
    cellular hyperosmotic response GO:0071474 IDA
    negative regulation of pancreatic juice secretion GO:0090188 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    lymphocyte migration into lymph node GO:0097022 ISS
    membraneless organelle assembly GO:0140694 IDA
    regulation of sodium ion transmembrane transport GO:1902305 IEA
    negative regulation of leukocyte cell-cell adhesion GO:1903038 IMP
    negative regulation of protein localization to plasma membrane GO:1903077 ISS
    positive regulation of mitotic cytokinesis GO:1903490 IDA
    regulation of monoatomic cation transmembrane transport GO:1904062 IMP
    positive regulation of termination of RNA polymerase II transcription GO:1904595 IDA
    cellular response to chemokine GO:1990869 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    membrane GO:0016020 ISS
    protein-containing complex GO:0032991 IEA
    intracellular membraneless organelle GO:0043232 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified WNK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WNK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 AP2B1 163
Proximity Label-MS Homo sapiens
3 AAK1 22848
Affinity Capture-MS Homo sapiens
4 EIF3K 27335
Co-fractionation Homo sapiens
5 WNK1 65125
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 PFKP 5214
Two-hybrid Homo sapiens
7 YWHAE 7531
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 RPL13A 23521
Cross-Linking-MS (XL-MS) Homo sapiens
9 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
10 GAK 2580
Affinity Capture-MS Homo sapiens
11 CALM1 801
Affinity Capture-MS Homo sapiens
12 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
13 CHCHD4  
Affinity Capture-MS Homo sapiens
14 LONP2 83752
Affinity Capture-MS Homo sapiens
15 TSC22D4 81628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TNRC6A 27327
Two-hybrid Homo sapiens
17 COPS5 10987
Two-hybrid Homo sapiens
18 IMPDH2 3615
Proximity Label-MS Homo sapiens
19 RCBTB2  
Affinity Capture-MS Homo sapiens
20 YWHAG 7532
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TIAM1  
Affinity Capture-MS Homo sapiens
22 ATP8  
Two-hybrid Homo sapiens
23 YWHAH 7533
Affinity Capture-MS Homo sapiens
24 WNK4  
Cross-Linking-MS (XL-MS) Homo sapiens
25 GTSE1 51512
Affinity Capture-MS Homo sapiens
26 SIAH2 6478
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PFN1 5216
Proximity Label-MS Homo sapiens
30 ZYX 7791
Two-hybrid Homo sapiens
31 TOLLIP 54472
Proximity Label-MS Homo sapiens
32 FHL3 2275
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 KLHL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 TGM2 7052
Affinity Capture-MS Homo sapiens
35 GLIS2  
Two-hybrid Homo sapiens
36 SPATA1  
Affinity Capture-MS Homo sapiens
37 FAM96B 51647
Affinity Capture-MS Homo sapiens
38 UBE2I 7329
Biochemical Activity Homo sapiens
39 UBR5 51366
Affinity Capture-Western Homo sapiens
40 VCP 7415
Affinity Capture-MS Homo sapiens
41 SLC9A1 6548
Two-hybrid Homo sapiens
42 EIF2B2 8892
Co-fractionation Homo sapiens
43 YWHAQ 10971
Affinity Capture-MS Homo sapiens
44 PRKAR1B  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
45 AGPS 8540
Proximity Label-MS Homo sapiens
46 PICALM 8301
Affinity Capture-MS Homo sapiens
47 SERPINB13 5275
Affinity Capture-MS Homo sapiens
48 HAO2  
Affinity Capture-MS Homo sapiens
49 BAG3 9531
Two-hybrid Homo sapiens
50 ATXN1 6310
Two-hybrid Homo sapiens
51 HNRNPD 3184
Co-fractionation Homo sapiens
52 S100P 6286
Affinity Capture-MS Homo sapiens
53 XPO1 7514
Affinity Capture-MS Homo sapiens
54 BAG2 9532
Affinity Capture-MS Homo sapiens
55 PRKAR1A 5573
Two-hybrid Homo sapiens
56 YWHAZ 7534
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
57 METTL21B  
Affinity Capture-MS Homo sapiens
58 Max  
Affinity Capture-MS Mus musculus
59 CDC42 998
Affinity Capture-MS Homo sapiens
60 MLF1  
Affinity Capture-MS Homo sapiens
61 RANBP9 10048
Two-hybrid Homo sapiens
62 MYC  
Dosage Lethality Homo sapiens
63 BAG1 573
Affinity Capture-MS Homo sapiens
64 HSPA1A 3303
Affinity Capture-MS Homo sapiens
65 PTPN23 25930
Proximity Label-MS Homo sapiens
66 PVR 5817
Two-hybrid Homo sapiens
67 CLTC 1213
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
68 CSDE1 7812
Two-hybrid Homo sapiens
69 STAMBPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ARAF 369
Two-hybrid Homo sapiens
71 UPF2 26019
Two-hybrid Homo sapiens
72 FHL2 2274
Affinity Capture-MS Homo sapiens
73 NEDD4L 23327
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
74 BAG4  
Affinity Capture-MS Homo sapiens
75 KLHL2 11275
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CD46 4179
Co-fractionation Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 FASN 2194
Positive Genetic Homo sapiens
79 SGK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 PLEC 5339
Affinity Capture-MS Homo sapiens
81 OXSR1 9943
Affinity Capture-MS Homo sapiens
82 MAP2K1 5604
Two-hybrid Homo sapiens
83 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 METTL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 GSTM3 2947
Affinity Capture-MS Homo sapiens
86 E2F3  
Two-hybrid Homo sapiens
87 Uso1 56041
Affinity Capture-MS Mus musculus
88 WNK3  
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
89 TSC22D1 8848
Two-hybrid Homo sapiens
90 ZNF106  
Two-hybrid Homo sapiens
91 RAPGEF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 CLTA 1211
Proximity Label-MS Homo sapiens
93 FLNC 2318
Two-hybrid Homo sapiens
94 RANGRF  
Affinity Capture-MS Homo sapiens
95 WNK2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
96 AAMP 14
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 RPA3 6119
Proximity Label-MS Homo sapiens
98 RANBP2 5903
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WNK1 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Stimuli-sensing channels TAS Reactome
Transport of small molecules TAS Reactome





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