Gene description for WNK1
Gene name WNK lysine deficient protein kinase 1
Gene symbol WNK1
Other names/aliases HSAN2
HSN2
KDP
PPP1R167
PRKWNK1
PSK
p65
Species Homo sapiens
 Database cross references - WNK1
ExoCarta ExoCarta_65125
Entrez Gene 65125
HGNC 14540
MIM 605232
UniProt Q9H4A3  
 WNK1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for WNK1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 TAS
    phosphatase binding GO:0019902 IDA
    chloride channel inhibitor activity GO:0019869 IDA
    ATP binding GO:0005524 IDA
    protein kinase inhibitor activity GO:0004860 IEA
    protein binding GO:0005515 IPI
Biological Process
    ion transport GO:0006811 ISS
    negative regulation of protein kinase activity GO:0006469 IEA
    regulation of cellular process GO:0050794 ISS
    peptidyl-threonine phosphorylation GO:0018107 TAS
    negative regulation of phosphatase activity GO:0010923 IDA
    intracellular signal transduction GO:0035556 TAS
    protein phosphorylation GO:0006468 IDA
    neuron development GO:0048666 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified WNK1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for WNK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 E2F3  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WNK1 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here