Gene description for MAP2K1
Gene name mitogen-activated protein kinase kinase 1
Gene symbol MAP2K1
Other names/aliases CFC3
MAPKK1
MEK1
MKK1
PRKMK1
Species Homo sapiens
 Database cross references - MAP2K1
ExoCarta ExoCarta_5604
Entrez Gene 5604
HGNC 6840
MIM 176872
UniProt Q02750  
 MAP2K1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MAP2K1
Molecular Function
    protein C-terminus binding GO:0008022 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 TAS
    Ras GTPase binding GO:0017016 IEA
    MAP kinase kinase activity GO:0004708 IDA
    protein serine/threonine/tyrosine kinase activity GO:0004712 TAS
    protein serine/threonine kinase activator activity GO:0043539 IDA
    ATP binding GO:0005524 IEA
    receptor signaling protein tyrosine phosphatase activity GO:0004728 IEA
    protein tyrosine kinase activity GO:0004713 IEA
    protein N-terminus binding GO:0047485 IDA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IEA
Biological Process
    labyrinthine layer development GO:0060711 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    positive regulation of gene expression GO:0010628 IMP
    regulation of vascular smooth muscle contraction GO:0003056 IEA
    regulation of early endosome to late endosome transport GO:2000641 TAS
    axon guidance GO:0007411 TAS
    negative regulation of homotypic cell-cell adhesion GO:0034111 IEA
    cell cycle arrest GO:0007050 IMP
    regulation of Golgi inheritance GO:0090170 TAS
    innate immune response GO:0045087 TAS
    chemotaxis GO:0006935 TAS
    response to glucocorticoid GO:0051384 IEA
    positive regulation of protein serine/threonine kinase activity GO:0071902 IDA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    vesicle transport along microtubule GO:0047496 IEA
    signal transduction GO:0007165 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    movement of cell or subcellular component GO:0006928 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    Ras protein signal transduction GO:0007265 TAS
    positive regulation of cell migration GO:0030335 IEA
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of cell differentiation GO:0045597 IEA
    Golgi inheritance GO:0048313 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    response to oxidative stress GO:0006979 IEA
    cellular senescence GO:0090398 IMP
    positive regulation of Ras protein signal transduction GO:0046579 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    MAPK cascade GO:0000165 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    activation of MAPKK activity GO:0000186 TAS
    neuron differentiation GO:0030182 IBA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    negative regulation of cell proliferation GO:0008285 IDA
    activation of MAPK activity GO:0000187 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    cell motility GO:0048870 IEA
    positive regulation of transcription elongation from RNA polymerase II promoter GO:0032968 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    keratinocyte differentiation GO:0030216 IEA
    response to axon injury GO:0048678 IEA
    protein heterooligomerization GO:0051291 IEA
    melanosome transport GO:0032402 IEA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    mitotic nuclear division GO:0007067 IEA
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    cell proliferation GO:0008283 IEA
    placenta blood vessel development GO:0060674 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
Subcellular Localization
    microtubule GO:0005874 IEA
    extracellular exosome GO:0070062 IDA
    dendrite cytoplasm GO:0032839 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    axon GO:0030424 IEA
    focal adhesion GO:0005925 TAS
    endoplasmic reticulum GO:0005783 IDA
    nucleus GO:0005634 TAS
    cytosol GO:0005829 TAS
    early endosome GO:0005769 TAS
    intracellular GO:0005622 IBA
    microtubule organizing center GO:0005815 IEA
    Golgi apparatus GO:0005794 TAS
    cell cortex GO:0005938 IEA
    late endosome GO:0005770 TAS
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 TAS
    perikaryon GO:0043204 IEA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified MAP2K1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 PEBP4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 MAPK8IP3  
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 GRB10 2887
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 Flna 192176
Two-hybrid Mus musculus
6 BRAF  
Invivo Homo sapiens
7 VAV1 7409
Affinity Capture-Western Homo sapiens
8 MAPK3 5595
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
9 HRAS 3265
Affinity Capture-Western Homo sapiens
10 MAPBPIP  
Affinity Capture-Western Homo sapiens
11 MAP3K4  
Invitro Homo sapiens
Invivo Homo sapiens
12 MAP3K8  
Biochemical Activity Homo sapiens
13 KSR2  
Affinity Capture-Western Homo sapiens
14 RAF1 5894
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
15 PEBP1 5037
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 LAMTOR3 8649
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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