Gene description for Flna
Gene name filamin, alpha
Gene symbol Flna
Other names/aliases ABP-280
Dilp2
F730004A14Rik
Fln1
GENA 379
filamin-1
Species Mus musculus
 Database cross references - Flna
ExoCarta ExoCarta_192176
Vesiclepedia VP_192176
Entrez Gene 192176
UniProt Q8BTM8  
 Flna identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Flna
Molecular Function
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 ISO
    Rho GTPase binding GO:0017048 ISO
    Rac GTPase binding GO:0048365 ISO
    signal transducer activity GO:0004871 ISO
    actin binding GO:0003779 TAS
    poly(A) RNA binding GO:0044822 ISO
    Ral GTPase binding GO:0017160 ISO
    SMAD binding GO:0046332 ISO
    transcription factor binding GO:0008134 ISO
    Fc-gamma receptor I complex binding GO:0034988 ISO
    protein homodimerization activity GO:0042803 ISO
    small GTPase binding GO:0031267 ISO
    protein kinase C binding GO:0005080 IDA
    actin filament binding GO:0051015 ISO
    mu-type opioid receptor binding GO:0031852 ISO
    glycoprotein binding GO:0001948 ISO
Biological Process
    adenylate cyclase-inhibiting dopamine receptor signaling pathway GO:0007195 ISO
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 ISO
    establishment of protein localization GO:0045184 ISO
    mitotic spindle assembly GO:0090307 ISO
    actin crosslink formation GO:0051764 ISO
    negative regulation of transcription from RNA polymerase I promoter GO:0016479 ISO
    positive regulation of integrin-mediated signaling pathway GO:2001046 ISO
    receptor clustering GO:0043113 ISO
    actin cytoskeleton organization GO:0030036 IGI
    protein stabilization GO:0050821 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    epithelial to mesenchymal transition GO:0001837 IGI
    negative regulation of protein catabolic process GO:0042177 ISO
    early endosome to late endosome transport GO:0045022 IDA
    actin cytoskeleton reorganization GO:0031532 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    protein localization to cell surface GO:0034394 ISO
    cytoplasmic sequestering of protein GO:0051220 ISO
    positive regulation of transcription factor import into nucleus GO:0042993 ISO
    platelet aggregation GO:0070527 ISO
    mRNA transcription from RNA polymerase II promoter GO:0042789 ISO
    cilium assembly GO:0042384 ISO
    cell projection organization GO:0030030 IEA
Subcellular Localization
    trans-Golgi network GO:0005802 IDA
    cytoskeleton GO:0005856 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    Myb complex GO:0031523 ISO
    cortical cytoskeleton GO:0030863 ISO
    nucleolar part GO:0044452 ISO
    apical dendrite GO:0097440 ISO
    plasma membrane GO:0005886 ISO
    protein complex GO:0043234 ISO
    cytoplasm GO:0005737 ISO
    actin filament bundle GO:0032432 IDA
    actin filament GO:0005884 ISO
    brush border GO:0005903 IDA
    neuronal cell body GO:0043025 ISO
    dendritic shaft GO:0043198 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    actin cytoskeleton GO:0015629 ISO
    cytosol GO:0005829 ISO
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Flna in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Flna
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K4 6416
Two-hybrid Homo sapiens
2 MAP2K2 5605
Two-hybrid Homo sapiens
3 FURIN 5045
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 MAP2K1 5604
Two-hybrid Homo sapiens
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