Gene description for MAP2K4
Gene name mitogen-activated protein kinase kinase 4
Gene symbol MAP2K4
Other names/aliases JNKK
JNKK1
MAPKK4
MEK4
MKK4
PRKMK4
SAPKK-1
SAPKK1
SEK1
SERK1
SKK1
Species Homo sapiens
 Database cross references - MAP2K4
ExoCarta ExoCarta_6416
Vesiclepedia VP_6416
Entrez Gene 6416
HGNC 6844
MIM 601335
UniProt P45985  
 MAP2K4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MAP2K4
Molecular Function
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
    ATP binding GO:0005524 IEA
    JUN kinase kinase activity GO:0008545 IBA
    protein tyrosine kinase activity GO:0004713 IEA
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 TAS
Biological Process
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    JNK cascade GO:0007254 TAS
    positive regulation of neuron apoptotic process GO:0043525 IEA
    stress-activated MAPK cascade GO:0051403 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    innate immune response GO:0045087 TAS
    cellular response to sorbitol GO:0072709 IEA
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    positive regulation of DNA replication GO:0045740 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    signal transduction GO:0007165 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    cell growth involved in cardiac muscle cell development GO:0061049 IEA
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 IEA
    apoptotic process GO:0006915 IEA
    activation of JUN kinase activity GO:0007257 IBA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
Subcellular Localization
    intracellular GO:0005622 IBA
    axon GO:0030424 IEA
    cytosol GO:0005829 TAS
    dendrite cytoplasm GO:0032839 IEA
    nucleus GO:0005634 IEA
    perikaryon GO:0043204 IEA
 Experiment description of studies that identified MAP2K4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K7 5609
Phenotypic Enhancement Homo sapiens
2 FLNC 2318
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 MAP4K2  
Reconstituted Complex Homo sapiens
4 MAPK8IP3  
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
5 MAP3K1 4214
Invivo Homo sapiens
6 Flna 192176
Two-hybrid Mus musculus
7 ARRB2 409
Invivo Homo sapiens
Invitro Homo sapiens
8 MAPK14 1432
Invitro Homo sapiens
9 AKT1 207
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
10 MAP3K2 10746
Invitro Homo sapiens
11 MAPK8 5599
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 MAP3K12  
Invitro Homo sapiens
13 MAP3K10  
Invitro Homo sapiens
14 MAP3K8  
Biochemical Activity Homo sapiens
15 MAP3K4 4216
Invitro Homo sapiens
Invivo Homo sapiens
16 SPAG9 9043
Affinity Capture-Western Homo sapiens
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