Gene description for ARRB2
Gene name arrestin, beta 2
Gene symbol ARRB2
Other names/aliases ARB2
ARR2
BARR2
Species Homo sapiens
 Database cross references - ARRB2
ExoCarta ExoCarta_409
Vesiclepedia VP_409
Entrez Gene 409
HGNC 712
MIM 107941
UniProt P32121  
 ARRB2 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ARRB2
Molecular Function
    follicle-stimulating hormone receptor binding GO:0031762 IEA
    enzyme binding GO:0019899 IPI
    alpha-1A adrenergic receptor binding GO:0031691 IEA
    14-3-3 protein binding GO:0071889 IEA
    protein domain specific binding GO:0019904 IEA
    type 1 angiotensin receptor binding GO:0031702 IEA
    G-protein coupled receptor binding GO:0001664 IPI
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    receptor binding GO:0005102 IPI
    protein complex scaffold GO:0032947 IDA
    type 2A serotonin receptor binding GO:0031826 IEA
    protein complex binding GO:0032403 IEA
    protein kinase B binding GO:0043422 IEA
    alpha-1B adrenergic receptor binding GO:0031692 IEA
    D1 dopamine receptor binding GO:0031748 IEA
    mitogen-activated protein kinase binding GO:0051019 IEA
    angiotensin receptor binding GO:0031701 IPI
Biological Process
    positive regulation of synaptic transmission, dopaminergic GO:0032226 IEA
    G-protein coupled receptor internalization GO:0002031 IMP
    negative regulation of protein ubiquitination GO:0031397 IDA
    protein ubiquitination GO:0016567 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    positive regulation of protein ubiquitination GO:0031398 IGI
    negative regulation of interleukin-1 beta production GO:0032691 IEA
    platelet activation GO:0030168 TAS
    receptor internalization GO:0031623 IDA
    regulation of androgen receptor signaling pathway GO:0060765 IDA
    negative regulation of GTPase activity GO:0034260 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IEA
    positive regulation of protein kinase B signaling GO:0051897 IEA
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IEA
    negative regulation of interleukin-6 production GO:0032715 IEA
    adult walking behavior GO:0007628 IEA
    protein transport GO:0015031 IEA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    follicle-stimulating hormone signaling pathway GO:0042699 IEA
    negative regulation of smooth muscle cell apoptotic process GO:0034392 IEA
    positive regulation of DNA biosynthetic process GO:2000573 IEA
    brain development GO:0007420 IEA
    positive regulation of receptor internalization GO:0002092 IMP
    Notch signaling pathway GO:0007219 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    cell chemotaxis GO:0060326 IMP
    negative regulation of toll-like receptor signaling pathway GO:0034122 IEA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IEA
    negative regulation of tumor necrosis factor production GO:0032720 IEA
    transcription from RNA polymerase II promoter GO:0006366 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of interleukin-12 production GO:0032695 IEA
    positive regulation of calcium ion transport GO:0051928 IEA
    negative regulation of natural killer cell mediated cytotoxicity GO:0045953 IMP
    desensitization of G-protein coupled receptor protein signaling pathway by arrestin GO:0002032 IMP
    detection of temperature stimulus involved in sensory perception of pain GO:0050965 IEA
    blood coagulation GO:0007596 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    dendritic spine GO:0043197 IEA
    postsynaptic density GO:0014069 IEA
    plasma membrane GO:0005886 TAS
    endocytic vesicle GO:0030139 IDA
    coated pit GO:0005905 IEA
    cytoplasmic vesicle GO:0031410 IDA
    cytosol GO:0005829 TAS
    basolateral plasma membrane GO:0016323 IEA
    cytoplasm GO:0005737 IDA
    postsynaptic membrane GO:0045211 IEA
 Experiment description of studies that identified ARRB2 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARRB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPL44  
Affinity Capture-MS Homo sapiens
2 KPNA3 3839
Affinity Capture-MS Homo sapiens
3 MAP2K4 6416
Invivo Homo sapiens
Invitro Homo sapiens
4 RALGDS  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 PES1 23481
Affinity Capture-MS Homo sapiens
6 TCOF1 6949
Affinity Capture-MS Homo sapiens
7 MRPL43 84545
Affinity Capture-MS Homo sapiens
8 BOP1 23246
Affinity Capture-MS Homo sapiens
9 MED8  
Two-hybrid Homo sapiens
10 PDE4D  
Affinity Capture-MS Homo sapiens
11 OPRD1  
Reconstituted Complex Homo sapiens
12 CYTH2 9266
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
13 ARF6 382
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
14 RPL7L1 285855
Affinity Capture-MS Homo sapiens
15 MAPK10 5602
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
16 KPNA4 3840
Affinity Capture-MS Homo sapiens
17 ARRB1 408
Affinity Capture-MS Homo sapiens
18 NOLC1 9221
Affinity Capture-MS Homo sapiens
19 AP1B1 162
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 MAPK3 5595
Affinity Capture-Western Homo sapiens
21 STC2 8614
Two-hybrid Homo sapiens
22 RAB5C 5878
Affinity Capture-MS Homo sapiens
23 FBL 2091
Affinity Capture-MS Homo sapiens
24 AP3B1 8546
Affinity Capture-MS Homo sapiens
25 DDX27 55661
Affinity Capture-MS Homo sapiens
26 MDM2  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 PTAFR  
Invivo Homo sapiens
Invitro Homo sapiens
28 AVPR2  
Affinity Capture-Western Homo sapiens
29 SMARCC2 6601
Two-hybrid Homo sapiens
View the network image/svg+xml



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