Gene description for ARRB2
Gene name arrestin, beta 2
Gene symbol ARRB2
Other names/aliases ARB2
ARR2
BARR2
Species Homo sapiens
 Database cross references - ARRB2
ExoCarta ExoCarta_409
Vesiclepedia VP_409
Entrez Gene 409
HGNC 712
MIM 107941
UniProt P32121  
 ARRB2 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ARRB2
Molecular Function
    G protein-coupled receptor binding GO:0001664 IPI
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    angiotensin receptor binding GO:0031701 IBA
    angiotensin receptor binding GO:0031701 IPI
    D1 dopamine receptor binding GO:0031748 IPI
    protein kinase B binding GO:0043422 IEA
    molecular adaptor activity GO:0060090 IDA
Biological Process
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 IMP
    G protein-coupled receptor internalization GO:0002031 IBA
    G protein-coupled receptor internalization GO:0002031 IDA
    G protein-coupled receptor internalization GO:0002031 IMP
    G protein-coupled receptor internalization GO:0002031 TAS
    positive regulation of receptor internalization GO:0002092 IMP
    transcription by RNA polymerase II GO:0006366 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 NAS
    adult walking behavior GO:0007628 IEA
    positive regulation of gene expression GO:0010628 IEA
    protein transport GO:0015031 IEA
    protein ubiquitination GO:0016567 IMP
    negative regulation of protein ubiquitination GO:0031397 IDA
    receptor internalization GO:0031623 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    positive regulation of synaptic transmission, dopaminergic GO:0032226 IEA
    negative regulation of interleukin-1 beta production GO:0032691 IEA
    negative regulation of interleukin-12 production GO:0032695 IEA
    negative regulation of interleukin-6 production GO:0032715 IEA
    negative regulation of tumor necrosis factor production GO:0032720 IEA
    negative regulation of toll-like receptor signaling pathway GO:0034122 IEA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    negative regulation of natural killer cell mediated cytotoxicity GO:0045953 IMP
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 NAS
    excitatory postsynaptic potential GO:0060079 NAS
    cell chemotaxis GO:0060326 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    postsynaptic signal transduction GO:0098926 IEA
    positive regulation of cardiac muscle cell differentiation GO:2000727 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IC
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    clathrin-coated pit GO:0005905 IEA
    endocytic vesicle GO:0030139 IDA
    endocytic vesicle membrane GO:0030666 TAS
    cytoplasmic vesicle GO:0031410 IDA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified ARRB2 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARRB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mdm2  
Affinity Capture-Western Mus musculus
2 EIF2AK4 440275
Affinity Capture-MS Homo sapiens
3 PPP2R4 5524
Affinity Capture-Western Homo sapiens
4 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
5 HDAC2 3066
Affinity Capture-MS Homo sapiens
6 PKM 5315
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 SRPK2 6733
Affinity Capture-MS Homo sapiens
8 TPTE2  
Affinity Capture-MS Homo sapiens
9 JPH1 56704
Affinity Capture-MS Homo sapiens
10 ACTC1 70
Affinity Capture-MS Homo sapiens
11 MIIP 60672
Affinity Capture-MS Homo sapiens
12 ADRB1  
Affinity Capture-Western Homo sapiens
13 BRD2  
Affinity Capture-MS Homo sapiens
14 UBC 7316
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
15 CSNK2A1 1457
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
16 WDR26 80232
Affinity Capture-MS Homo sapiens
17 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 AFF4 27125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ADGRG1 9289
Affinity Capture-Western Homo sapiens
20 C18orf25 147339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 AP2A1 160
Affinity Capture-MS Homo sapiens
22 DGKE  
Affinity Capture-MS Homo sapiens
23 ALPK2  
Affinity Capture-MS Homo sapiens
24 SRC 6714
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PPM1A 5494
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 USP33  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
28 RPL19 6143
Affinity Capture-MS Homo sapiens
29 MYL6 4637
Affinity Capture-MS Homo sapiens
30 PRM3  
Affinity Capture-MS Homo sapiens
31 PPM1B 5495
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
32 BRPF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TCOF1 6949
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 OPRD1  
Reconstituted Complex Homo sapiens
35 CAPZA1 829
Affinity Capture-MS Homo sapiens
36 MAP1B 4131
Affinity Capture-MS Homo sapiens
37 PABPC1 26986
Affinity Capture-MS Homo sapiens
38 MYH9 4627
Affinity Capture-MS Homo sapiens
39 RPL31 6160
Affinity Capture-MS Homo sapiens
40 XRCC5 7520
Affinity Capture-MS Homo sapiens
41 P4HB 5034
Affinity Capture-MS Homo sapiens
42 SNRPD1 6632
Affinity Capture-MS Homo sapiens
43 PAK7  
Affinity Capture-MS Homo sapiens
44 CAPN1 823
Affinity Capture-MS Homo sapiens
45 EPB41L5 57669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HCRTR1  
FRET Homo sapiens
47 KBTBD8  
Affinity Capture-Western Homo sapiens
48 BCLAF1 9774
Affinity Capture-MS Homo sapiens
49 HSPA5 3309
Affinity Capture-MS Homo sapiens
50 AHCYL1 10768
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ARRDC4  
Affinity Capture-Western Homo sapiens
52 CSN2 1447
Affinity Capture-MS Homo sapiens
53 CUL3 8452
Affinity Capture-Western Homo sapiens
54 RPL7L1 285855
Affinity Capture-MS Homo sapiens
55 TBXA2R 6915
Affinity Capture-Western Homo sapiens
56 MYO1C 4641
Affinity Capture-MS Homo sapiens
57 HIST1H2BO 8348
Affinity Capture-MS Homo sapiens
58 CDC42 998
Two-hybrid Homo sapiens
59 TUBA4A 7277
Affinity Capture-MS Homo sapiens
60 PRMT5 10419
Affinity Capture-MS Homo sapiens
61 STAT1 6772
Affinity Capture-MS Homo sapiens
62 AP2A2 161
Affinity Capture-MS Homo sapiens
63 RPL35A 6165
Affinity Capture-MS Homo sapiens
64 ZNF653  
Affinity Capture-MS Homo sapiens
65 EDNRA 1909
Affinity Capture-Western Homo sapiens
66 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
67 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CTNNA1 1495
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
69 TRPV4 59341
Affinity Capture-MS Homo sapiens
70 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
71 DDX1 1653
Affinity Capture-MS Homo sapiens
72 PABPC3 5042
Affinity Capture-MS Homo sapiens
73 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
74 STUB1 10273
Affinity Capture-Western Homo sapiens
75 YBX1 4904
Affinity Capture-MS Homo sapiens
76 EIF1AD 84285
Affinity Capture-MS Homo sapiens
77 RPS27A 6233
Affinity Capture-MS Homo sapiens
78 FASN 2194
Affinity Capture-MS Homo sapiens
79 SF3B1 23451
Affinity Capture-MS Homo sapiens
80 WDR77 79084
Affinity Capture-MS Homo sapiens
81 RPL6 6128
Affinity Capture-MS Homo sapiens
82 CYTH1 9267
Affinity Capture-Western Homo sapiens
83 G3BP2 9908
Affinity Capture-MS Homo sapiens
84 SIRT1  
Affinity Capture-MS Homo sapiens
85 BOLA2 552900
Affinity Capture-MS Homo sapiens
86 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
87 PHKA2 5256
Affinity Capture-MS Homo sapiens
88 GAPDH 2597
Affinity Capture-MS Homo sapiens
89 ALYREF 10189
Affinity Capture-MS Homo sapiens
90 CDK4 1019
Affinity Capture-MS Homo sapiens
91 CLTA 1211
Affinity Capture-MS Homo sapiens
92 EPHA2 1969
Proximity Label-MS Homo sapiens
93 PTGES3 10728
Affinity Capture-Western Homo sapiens
94 CAMK2D 817
Affinity Capture-MS Homo sapiens
95 RAB5C 5878
Affinity Capture-MS Homo sapiens
96 ANXA2 302
Affinity Capture-MS Homo sapiens
97 C16orf71  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 PTCH1  
Affinity Capture-MS Homo sapiens
99 NOP10 55505
Affinity Capture-MS Homo sapiens
100 HSPA1B 3304
Affinity Capture-MS Homo sapiens
101 RANGAP1 5905
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
102 CTNND1 1500
Affinity Capture-MS Homo sapiens
103 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
104 CENPB 1059
Affinity Capture-MS Homo sapiens
105 MCM3 4172
Affinity Capture-MS Homo sapiens
106 TUBA8 51807
Affinity Capture-MS Homo sapiens
107 KPNA3 3839
Affinity Capture-MS Homo sapiens
108 CDK3 1018
Affinity Capture-MS Homo sapiens
109 HSPA6 3310
Affinity Capture-MS Homo sapiens
110 RPS17 6218
Affinity Capture-MS Homo sapiens
111 ADRB2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
112 DBN1 1627
Affinity Capture-MS Homo sapiens
113 NOP56 10528
Affinity Capture-MS Homo sapiens
114 RPL14 9045
Affinity Capture-MS Homo sapiens
115 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
116 IGFALS 3483
Co-fractionation Homo sapiens
117 DIEXF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 CLTB 1212
Affinity Capture-MS Homo sapiens
119 CCDC102B  
Affinity Capture-MS Homo sapiens
120 MAPK10 5602
Affinity Capture-Western Homo sapiens
121 RASA2 5922
Affinity Capture-MS Homo sapiens
122 NOLC1 9221
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 H1FX 8971
Affinity Capture-MS Homo sapiens
124 SPIN1  
Affinity Capture-MS Homo sapiens
125 NPM1 4869
Affinity Capture-MS Homo sapiens
126 SRRM2 23524
Affinity Capture-MS Homo sapiens
127 YWHAH 7533
Affinity Capture-MS Homo sapiens
128 CTPS1 1503
Affinity Capture-MS Homo sapiens
129 SPIN3  
Affinity Capture-MS Homo sapiens
130 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 PES1 23481
Affinity Capture-MS Homo sapiens
132 Trhr  
FRET Rattus norvegicus
133 MAPK3 5595
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
134 HNRNPA0 10949
Affinity Capture-MS Homo sapiens
135 MCC 4163
Affinity Capture-MS Homo sapiens
136 RPL4 6124
Affinity Capture-MS Homo sapiens
137 SLC9A5  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
138 IGKV1-5 28299
Affinity Capture-MS Homo sapiens
139 RPL35 11224
Affinity Capture-MS Homo sapiens
140 UBR5 51366
Affinity Capture-MS Homo sapiens
141 TUBA1C 84790
Affinity Capture-MS Homo sapiens
142 LHCGR  
Reconstituted Complex Homo sapiens
143 RAD18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 NCL 4691
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
145 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
146 GPBP1L1  
Affinity Capture-MS Homo sapiens
147 RPS6 6194
Affinity Capture-MS Homo sapiens
148 RPLP0 6175
Affinity Capture-MS Homo sapiens
149 CUL5 8065
Affinity Capture-MS Homo sapiens
150 DDX27 55661
Affinity Capture-MS Homo sapiens
151 EIF4B 1975
Affinity Capture-MS Homo sapiens
152 HNRNPD 3184
Affinity Capture-MS Homo sapiens
153 MRPL44  
Affinity Capture-MS Homo sapiens
154 RPN2 6185
Affinity Capture-MS Homo sapiens
155 LEO1 123169
Affinity Capture-MS Homo sapiens
156 XPO1 7514
Affinity Capture-MS Homo sapiens
157 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 POLR2E 5434
Affinity Capture-MS Homo sapiens
159 TUBB4B 10383
Affinity Capture-MS Homo sapiens
160 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
161 ANKRD11 29123
Affinity Capture-MS Homo sapiens
162 WEE1 7465
Affinity Capture-MS Homo sapiens
163 TMEM169  
Affinity Capture-MS Homo sapiens
164 SCYL2 55681
Affinity Capture-MS Homo sapiens
165 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
166 EFTUD2 9343
Affinity Capture-MS Homo sapiens
167 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
168 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
169 CSNK2A2 1459
Biochemical Activity Homo sapiens
170 TMOD3 29766
Affinity Capture-MS Homo sapiens
171 Mlnr  
FRET Rattus norvegicus
172 PRPF4 9128
Affinity Capture-Western Homo sapiens
173 EEF1A2 1917
Affinity Capture-MS Homo sapiens
174 EVI2A  
Affinity Capture-MS Homo sapiens
175 TMEM74  
Affinity Capture-MS Homo sapiens
176 RPL26 6154
Affinity Capture-MS Homo sapiens
177 AVPR2  
Affinity Capture-Western Homo sapiens
178 EEF1A1 1915
Affinity Capture-MS Homo sapiens
179 SPTBN1 6711
Affinity Capture-MS Homo sapiens
180 HNRNPH2 3188
Affinity Capture-MS Homo sapiens
181 ILF3 3609
Affinity Capture-MS Homo sapiens
182 MAP2K4 6416
Affinity Capture-Western Homo sapiens
183 TMEM38A 79041
Affinity Capture-MS Homo sapiens
184 KANK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
185 ARR3 407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 PTBP1 5725
Affinity Capture-MS Homo sapiens
187 CENPF 1063
Affinity Capture-MS Homo sapiens
188 MAP2K1 5604
Affinity Capture-Western Homo sapiens
189 HNRNPK 3190
Affinity Capture-MS Homo sapiens
190 POU2F1 5451
Affinity Capture-MS Homo sapiens
191 DCD 117159
Affinity Capture-MS Homo sapiens
192 AHCYL2 23382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 YBX3 8531
Affinity Capture-MS Homo sapiens
194 RPL7A 6130
Affinity Capture-MS Homo sapiens
195 C1QBP 708
Affinity Capture-MS Homo sapiens
196 LRP11  
Affinity Capture-MS Homo sapiens
197 AP3D1 8943
Affinity Capture-MS Homo sapiens
198 KLHL12  
Affinity Capture-Western Homo sapiens
199 PRMT1 3276
Affinity Capture-MS Homo sapiens
200 LURAP1  
Affinity Capture-MS Homo sapiens
201 RANBP2 5903
Affinity Capture-Western Homo sapiens
202 HNRNPC 3183
Affinity Capture-MS Homo sapiens
203 YWHAE 7531
Affinity Capture-MS Homo sapiens
204 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
205 COPRS  
Affinity Capture-MS Homo sapiens
206 BOLA2B 654483
Affinity Capture-MS Homo sapiens
207 RPS19 6223
Affinity Capture-MS Homo sapiens
208 SYNE2 23224
Affinity Capture-MS Homo sapiens
209 HNRNPR 10236
Affinity Capture-MS Homo sapiens
210 OXER1  
FRET Homo sapiens
211 TRIM28 10155
Affinity Capture-MS Homo sapiens
212 EMILIN1 11117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
213 MLLT4 4301
Affinity Capture-MS Homo sapiens
214 SENP1  
Affinity Capture-Western Homo sapiens
215 Naa10  
Affinity Capture-MS Mus musculus
216 GPR55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
217 SLC31A1 1317
Affinity Capture-MS Homo sapiens
218 SYT2  
Affinity Capture-MS Homo sapiens
219 ATG16L1 55054
Affinity Capture-MS Homo sapiens
220 PFKFB3 5209
Affinity Capture-MS Homo sapiens
221 ARPC5L 81873
Affinity Capture-MS Homo sapiens
222 PABPC4 8761
Affinity Capture-MS Homo sapiens
223 MAS1