Gene description for SAG
Gene name S-antigen; retina and pineal gland (arrestin)
Gene symbol SAG
Other names/aliases RP47
S-AG
Species Homo sapiens
 Database cross references - SAG
ExoCarta ExoCarta_6295
Vesiclepedia VP_6295
Entrez Gene 6295
HGNC 10521
MIM 181031
UniProt P10523  
 SAG identified in sEVs derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SAG
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    opsin binding GO:0002046 IEA
    protein phosphatase inhibitor activity GO:0004864 TAS
    protein binding GO:0005515 IPI
    phosphoprotein binding GO:0051219 IEA
Biological Process
    G protein-coupled receptor internalization GO:0002031 IBA
    cell surface receptor signaling pathway GO:0007166 TAS
    G protein-coupled opsin signaling pathway GO:0016056 TAS
Subcellular Localization
    photoreceptor outer segment GO:0001750 IBA
    photoreceptor outer segment GO:0001750 IDA
    photoreceptor inner segment GO:0001917 IBA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IEA
 Experiment description of studies that identified SAG in sEVs
1
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SAG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARRB2 409
Affinity Capture-MS Homo sapiens
2 SAG 6295
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RHO  
Reconstituted Complex Homo sapiens
5 CLK3  
Two-hybrid Homo sapiens
6 DDX58 23586
Affinity Capture-RNA Homo sapiens
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