Gene description for ARHGEF12
Gene name Rho guanine nucleotide exchange factor (GEF) 12
Gene symbol ARHGEF12
Other names/aliases LARG
Species Homo sapiens
 Database cross references - ARHGEF12
ExoCarta ExoCarta_23365
Entrez Gene 23365
HGNC 14193
MIM 604763
UniProt Q9NZN5  
 ARHGEF12 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for ARHGEF12
Molecular Function
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    G-protein coupled receptor binding GO:0001664 IDA
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
Biological Process
    G-protein coupled receptor signaling pathway GO:0007186 IDA
    apoptotic signaling pathway GO:0097190 TAS
    termination of G-protein coupled receptor signaling pathway GO:0038032 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    axon guidance GO:0007411 TAS
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IEA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified ARHGEF12 in exosomes
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ARHGEF12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNA13 10672
Invitro Homo sapiens
Invivo Homo sapiens
2 PLXNB2 23654
Affinity Capture-Western Homo sapiens
3 ARHGEF12 23365
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 GNA12 2768
Invitro Homo sapiens
Invivo Homo sapiens
5 RHOA 387
Reconstituted Complex Homo sapiens
6 PLXNB1 5364
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 IGF1R 3480
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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