Gene description for RCL1
Gene name RNA terminal phosphate cyclase-like 1
Gene symbol RCL1
Other names/aliases RNAC
RPCL1
Species Homo sapiens
 Database cross references - RCL1
ExoCarta ExoCarta_10171
Entrez Gene 10171
HGNC 17687
MIM 611405
UniProt Q9Y2P8  
 RCL1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for RCL1
Molecular Function
    RNA-3'-phosphate cyclase activity GO:0003963 IBA
    endoribonuclease activity GO:0004521 IBA
    RNA binding GO:0003723 IBA
Biological Process
    endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000479 IBA
    biological_process GO:0008150 ND
Subcellular Localization
    nucleolus GO:0005730 IBA
 Experiment description of studies that identified RCL1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RCL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RCL1 is involved
No pathways found





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