Gene description for RCL1
Gene name RNA terminal phosphate cyclase-like 1
Gene symbol RCL1
Other names/aliases RNAC
RPCL1
Species Homo sapiens
 Database cross references - RCL1
ExoCarta ExoCarta_10171
Vesiclepedia VP_10171
Entrez Gene 10171
HGNC 17687
MIM 611405
UniProt Q9Y2P8  
 RCL1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for RCL1
Molecular Function
    RNA endonuclease activity GO:0004521 IBA
    protein binding GO:0005515 IPI
Biological Process
    endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000447 IEA
    endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000479 IBA
    endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000480 IEA
    ribosomal small subunit biogenesis GO:0042274 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    small-subunit processome GO:0032040 IDA
 Experiment description of studies that identified RCL1 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RCL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 HERC1 8925
Affinity Capture-MS Homo sapiens
3 HECTD1 25831
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 Eif3i 54709
Affinity Capture-MS Mus musculus
6 POP1 10940
Affinity Capture-MS Homo sapiens
7 GEMIN7  
Affinity Capture-MS Homo sapiens
8 TTL 150465
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 ARHGEF12 23365
Affinity Capture-MS Homo sapiens
11 BMS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 HDGF 3068
Affinity Capture-MS Homo sapiens
13 DNAJC8 22826
Proximity Label-MS Homo sapiens
14 TOP1 7150
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 LMNB1 4001
Affinity Capture-MS Homo sapiens
17 RC3H2  
Affinity Capture-MS Homo sapiens
18 SERPINB9 5272
Affinity Capture-MS Homo sapiens
19 SIRT7  
Affinity Capture-MS Homo sapiens
20 LETM1 3954
Affinity Capture-MS Homo sapiens
21 IFI16 3428
Affinity Capture-MS Homo sapiens
22 USP36  
Affinity Capture-MS Homo sapiens
23 CTTN 2017
Affinity Capture-MS Homo sapiens
24 BRCA1 672
Affinity Capture-MS Homo sapiens
25 YY1 7528
Affinity Capture-MS Homo sapiens
26 EMB 133418
Affinity Capture-MS Homo sapiens
27 RPS19 6223
Affinity Capture-MS Homo sapiens
28 CHCHD1  
Proximity Label-MS Homo sapiens
29 MECP2 4204
Affinity Capture-MS Homo sapiens
30 C1orf35  
Affinity Capture-MS Homo sapiens
31 EGFR 1956
Negative Genetic Homo sapiens
32 PTPN2 5771
Affinity Capture-MS Homo sapiens
33 PAPD5 64282
Affinity Capture-MS Homo sapiens
34 TAF2  
Affinity Capture-MS Homo sapiens
35 PPP1R10  
Affinity Capture-MS Homo sapiens
36 Ncaph  
Affinity Capture-MS Mus musculus
37 SUZ12  
Affinity Capture-MS Homo sapiens
38 SART1 9092
Affinity Capture-MS Homo sapiens
39 TFG 10342
Affinity Capture-MS Homo sapiens
40 NXF1 10482
Affinity Capture-RNA Homo sapiens
41 DNAJB6 10049
Proximity Label-MS Homo sapiens
42 GOLGA2 2801
Two-hybrid Homo sapiens
43 UFL1 23376
Affinity Capture-MS Homo sapiens
44 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
45 FKBP5 2289
Affinity Capture-MS Homo sapiens
46 DDX56  
Affinity Capture-MS Homo sapiens
47 NSRP1  
Affinity Capture-MS Homo sapiens
48 MOV10 4343
Affinity Capture-RNA Homo sapiens
49 ULK3 25989
Affinity Capture-MS Homo sapiens
50 PSPC1 55269
Affinity Capture-MS Homo sapiens
51 HECW2  
Affinity Capture-MS Homo sapiens
52 RPSA 3921
Affinity Capture-MS Homo sapiens
53 NUFIP2 57532
Affinity Capture-MS Homo sapiens
54 PPIL4  
Affinity Capture-MS Homo sapiens
55 DNAJC9 23234
Proximity Label-MS Homo sapiens
56 CDK9 1025
Affinity Capture-MS Homo sapiens
57 ARHGAP11A  
Affinity Capture-MS Homo sapiens
58 CIT 11113
Affinity Capture-MS Homo sapiens
59 NCL 4691
Affinity Capture-MS Homo sapiens
60 DDX42 11325
Affinity Capture-MS Homo sapiens
61 CPSF7 79869
Affinity Capture-MS Homo sapiens
62 DDRGK1 65992
Affinity Capture-MS Homo sapiens
63 RNF2  
Affinity Capture-MS Homo sapiens
64 Tagap  
Affinity Capture-MS Mus musculus
65 USF1  
Affinity Capture-MS Homo sapiens
66 RC3H1 149041
Affinity Capture-MS Homo sapiens
67 CCNT1  
Affinity Capture-MS Homo sapiens
68 SSRP1 6749
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 APBB1  
Affinity Capture-MS Homo sapiens
71 KLHL21 9903
Affinity Capture-MS Homo sapiens
72 KRAS 3845
Negative Genetic Homo sapiens
73 NPM1 4869
Affinity Capture-MS Homo sapiens
74 PIN4 5303
Affinity Capture-MS Homo sapiens
75 EED  
Affinity Capture-MS Homo sapiens
76 NUSAP1 51203
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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