Gene description for Eif3i
Gene name eukaryotic translation initiation factor 3, subunit I
Gene symbol Eif3i
Other names/aliases 36kDa
D4Ertd632e
Eif3s2
Trip1
Species Mus musculus
 Database cross references - Eif3i
ExoCarta ExoCarta_54709
Vesiclepedia VP_54709
Entrez Gene 54709
UniProt Q9QZD9  
 Eif3i identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3i
Molecular Function
    RNA binding GO:0003723 IBA
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISO
    protein binding GO:0005515 IPI
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IEA
    cytoplasmic translational initiation GO:0002183 IBA
    translational initiation GO:0006413 IDA
    translational initiation GO:0006413 ISO
Subcellular Localization
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IBA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IDA
 Experiment description of studies that identified Eif3i in exosomes
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3i
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX4  
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 SUPT16H 11198
Affinity Capture-MS Homo sapiens
4 SPATS2  
Affinity Capture-MS Homo sapiens
5 UTP18  
Affinity Capture-MS Homo sapiens
6 EIF3F 8665
Affinity Capture-MS Homo sapiens
7 Lmna 16905
Affinity Capture-MS Mus musculus
8 CCT8 10694
Affinity Capture-MS Homo sapiens
9 PARP1 142
Affinity Capture-MS Homo sapiens
10 RPS3 6188
Affinity Capture-MS Homo sapiens
11 Eif5b 226982
Co-fractionation Mus musculus
12 MSH6 2956
Affinity Capture-MS Homo sapiens
13 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
14 EIF3C 8663
Affinity Capture-MS Homo sapiens
15 KIAA0020 9933
Affinity Capture-MS Homo sapiens
16 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
17 SNRPE 6635
Affinity Capture-MS Homo sapiens
18 RCL1 10171
Affinity Capture-MS Homo sapiens
19 PRRC2C 23215
Affinity Capture-MS Homo sapiens
20 MRPL38  
Affinity Capture-MS Homo sapiens
21 EIF3K 27335
Affinity Capture-MS Homo sapiens
22 RFC5 5985
Affinity Capture-MS Homo sapiens
23 Eif1 20918
Co-fractionation Mus musculus
24 Chaf1a  
Affinity Capture-MS Mus musculus
25 EIF3G 8666
Affinity Capture-MS Homo sapiens
26 Ncl 17975
Affinity Capture-Western Mus musculus
27 DHX30 22907
Affinity Capture-MS Homo sapiens
28 PRRC2B  
Affinity Capture-MS Homo sapiens
29 WDR18 57418
Affinity Capture-MS Homo sapiens
30 Lin28a  
Affinity Capture-Western Mus musculus
31 RFC1 5981
Affinity Capture-MS Homo sapiens
32 Mettl21e  
Affinity Capture-MS Mus musculus
33 STRAP 11171
Affinity Capture-MS Homo sapiens
34 Tmem173  
Proximity Label-MS Mus musculus
35 Fzr1  
Affinity Capture-MS Mus musculus
36 ITGAV 3685
Affinity Capture-MS Homo sapiens
37 TOP2A 7153
Affinity Capture-MS Homo sapiens
38 WDR76  
Affinity Capture-MS Homo sapiens
39 EIF3J 8669
Affinity Capture-MS Homo sapiens
40 HNRNPL 3191
Affinity Capture-MS Homo sapiens
41 DDX47 51202
Affinity Capture-MS Homo sapiens
42 H1FX 8971
Affinity Capture-MS Homo sapiens
43 RPS6 6194
Affinity Capture-MS Homo sapiens
44 RRP12 23223
Affinity Capture-MS Homo sapiens
45 EIF3B 8662
Affinity Capture-MS Homo sapiens
46 HIST2H3A 333932
Affinity Capture-MS Homo sapiens
47 RPL23A 6147
Affinity Capture-MS Homo sapiens
48 EIF4G2 1982
Affinity Capture-MS Homo sapiens
49 Eed  
Affinity Capture-MS Mus musculus
50 EIF3H 8667
Affinity Capture-MS Homo sapiens
51 PELP1 27043
Affinity Capture-MS Homo sapiens
52 TEX10 54881
Affinity Capture-MS Homo sapiens
53 Atg16l1  
Affinity Capture-MS Mus musculus
54 RPS15A 6210
Affinity Capture-MS Homo sapiens
55 EIF3E 3646
Affinity Capture-MS Homo sapiens
56 EIF3M 10480
Affinity Capture-MS Homo sapiens
57 GNL3 26354
Affinity Capture-MS Homo sapiens
58 DDX55  
Affinity Capture-MS Homo sapiens
59 EIF3D 8664
Affinity Capture-MS Homo sapiens
60 RPS16 6217
Affinity Capture-MS Homo sapiens
61 EIF3L 51386
Affinity Capture-MS Homo sapiens
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