Gene description for RFC1
Gene name replication factor C (activator 1) 1, 145kDa
Gene symbol RFC1
Other names/aliases A1
MHCBFB
PO-GA
RECC1
RFC
RFC140
Species Homo sapiens
 Database cross references - RFC1
ExoCarta ExoCarta_5981
Vesiclepedia VP_5981
Entrez Gene 5981
HGNC 9969
MIM 102579
UniProt P35251  
 RFC1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for RFC1
Molecular Function
    ATP binding GO:0005524 IEA
    DNA binding GO:0003677 IBA
    double-stranded DNA binding GO:0003690 IEA
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 TAS
    sequence-specific DNA binding GO:0043565 IEA
    DNA clamp loader activity GO:0003689 IEA
    protein domain specific binding GO:0019904 IEA
Biological Process
    error-prone translesion synthesis GO:0042276 TAS
    transcription, DNA-templated GO:0006351 IEA
    telomere maintenance via recombination GO:0000722 TAS
    DNA-dependent DNA replication GO:0006261 TAS
    DNA repair GO:0006281 TAS
    positive regulation of catalytic activity GO:0043085 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    mitotic cell cycle GO:0000278 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    error-free translesion synthesis GO:0070987 TAS
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    nucleotide-excision repair GO:0006289 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    telomere maintenance via telomerase GO:0007004 TAS
    translesion synthesis GO:0019985 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    base-excision repair GO:0006284 TAS
    telomere maintenance GO:0000723 TAS
Subcellular Localization
    DNA replication factor C complex GO:0005663 IDA
    nucleolus GO:0005730 IEA
    Golgi apparatus GO:0005794 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    extracellular exosome GO:0070062 IDA
    cell junction GO:0030054 IDA
 Experiment description of studies that identified RFC1 in exosomes
1
Experiment ID 234
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RFC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 RAD50 10111
Co-purification Homo sapiens
3 MSH6 2956
Co-purification Homo sapiens
4 TP53 7157
Affinity Capture-Western Homo sapiens
5 MSH2 4436
Co-purification Homo sapiens
6 RB1 5925
Affinity Capture-Western Homo sapiens
7 KIT 3815
Affinity Capture-Western Homo sapiens
8 MRE11A 4361
Co-purification Homo sapiens
9 MLH1 4292
Co-purification Homo sapiens
10 HDAC1 3065
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 BLM 641
Co-purification Homo sapiens
12 NBN 4683
Co-purification Homo sapiens
13 PCNA 5111
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 ATM 472
Co-purification Homo sapiens
15 CDK4 1019
Affinity Capture-Western Homo sapiens
16 CASP3 836
Invitro Homo sapiens
17 RFC3 5983
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
18 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 CCND1 595
Affinity Capture-Western Homo sapiens
20 BRCA1 672
Co-purification Homo sapiens
21 XRCC5 7520
Affinity Capture-Western Homo sapiens
22 POLD1 5424
Invivo Homo sapiens
Invitro Homo sapiens
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