Gene description for RFC1
Gene name replication factor C (activator 1) 1, 145kDa
Gene symbol RFC1
Other names/aliases A1
MHCBFB
PO-GA
RECC1
RFC
RFC140
Species Homo sapiens
 Database cross references - RFC1
ExoCarta ExoCarta_5981
Vesiclepedia VP_5981
Entrez Gene 5981
HGNC 9969
MIM 102579
UniProt P35251  
 RFC1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for RFC1
Molecular Function
    DNA binding GO:0003677 IBA
    DNA clamp loader activity GO:0003689 IEA
    double-stranded DNA binding GO:0003690 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme activator activity GO:0008047 TAS
    ATP hydrolysis activity GO:0016887 IEA
    protein domain specific binding GO:0019904 IEA
    sequence-specific DNA binding GO:0043565 IEA
    DNA clamp unloader activity GO:0061860 IBA
    DNA clamp unloader activity GO:0061860 IMP
    DNA-binding transcription factor binding GO:0140297 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    DNA-templated DNA replication GO:0006261 IDA
    DNA repair GO:0006281 IEA
    telomere maintenance via telomerase GO:0007004 TAS
    positive regulation of DNA-templated transcription GO:0045893 IEA
    DNA clamp unloading GO:0090618 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    DNA replication factor C complex GO:0005663 IDA
    DNA replication factor C complex GO:0005663 IPI
    Elg1 RFC-like complex GO:0031391 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified RFC1 in sEVs
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RFC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 ZNF668  
Affinity Capture-MS Homo sapiens
6 SENP3 26168
Affinity Capture-MS Homo sapiens
7 SPRTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PMS1  
Affinity Capture-Western Homo sapiens
9 MSH6 2956
Co-purification Homo sapiens
Co-fractionation Homo sapiens
10 ANLN 54443
Affinity Capture-MS Homo sapiens
11 RACGAP1 29127
Co-fractionation Homo sapiens
12 RPA2 6118
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
13 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
14 NOP56 10528
Affinity Capture-MS Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 BRD2  
Affinity Capture-MS Homo sapiens
17 UBC 7316
Reconstituted Complex Homo sapiens
18 LAMP2 3920
Co-fractionation Homo sapiens
19 CHEK2  
Synthetic Lethality Homo sapiens
20 FGF3  
Affinity Capture-MS Homo sapiens
21 SOX2  
Affinity Capture-MS Homo sapiens
22 CENPA  
Proximity Label-MS Homo sapiens
23 APEX1 328
Proximity Label-MS Homo sapiens
24 TOP2A 7153
Co-fractionation Homo sapiens
25 WDR76  
Affinity Capture-MS Homo sapiens
26 KIF23 9493
Affinity Capture-MS Homo sapiens
27 LIG1 3978
Co-fractionation Homo sapiens
28 FBXW7  
Affinity Capture-MS Homo sapiens
29 RFC3 5983
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
30 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 FBL 2091
Proximity Label-MS Homo sapiens
32 H2AFY2 55506
Affinity Capture-MS Homo sapiens
33 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SMC2 10592
Co-fractionation Homo sapiens
35 PRKRA 8575
Affinity Capture-MS Homo sapiens
36 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 RRP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 Eif3i 54709
Affinity Capture-MS Mus musculus
39 CUL1 8454
Affinity Capture-MS Homo sapiens
40 CSNK2B 1460
Two-hybrid Homo sapiens
41 OBSL1 23363
Affinity Capture-MS Homo sapiens
42 RBM14 10432
Co-fractionation Homo sapiens
43 ESR1  
Affinity Capture-MS Homo sapiens
44 HSPA8 3312
Affinity Capture-MS Homo sapiens
45 SMC4 10051
Co-fractionation Homo sapiens
46 TPT1 7178
Affinity Capture-MS Homo sapiens
47 POLA2  
Co-fractionation Homo sapiens
48 RB1 5925
Affinity Capture-Western Homo sapiens
49 E2F4  
Affinity Capture-MS Homo sapiens
50 H2AFZ 3015
Affinity Capture-MS Homo sapiens
51 NF2 4771
Affinity Capture-MS Homo sapiens
52 SRP9 6726
Affinity Capture-MS Homo sapiens
53 POLD1 5424
Co-fractionation Homo sapiens
54 C10orf12  
Affinity Capture-MS Homo sapiens
55 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
56 MRE11A 4361
Co-purification Homo sapiens
Co-fractionation Homo sapiens
57 PARK2  
Affinity Capture-MS Homo sapiens
58 BLM 641
Co-purification Homo sapiens
Co-fractionation Homo sapiens
59 NBN 4683
Co-purification Homo sapiens
Co-fractionation Homo sapiens
60 RFC5 5985
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
61 CBX3 11335
Proximity Label-MS Homo sapiens
62 RPL5 6125
Affinity Capture-MS Homo sapiens
63 AKR7L 246181
Affinity Capture-MS Homo sapiens
64 RPL18A 6142
Affinity Capture-MS Homo sapiens
65 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
66 PDS5A 23244
Co-fractionation Homo sapiens
67 NTRK1 4914
Affinity Capture-MS Homo sapiens
68 RPL31 6160
Proximity Label-MS Homo sapiens
69 IFI16 3428
Affinity Capture-MS Homo sapiens
70 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
71 CHEK1  
Synthetic Lethality Homo sapiens
72 TEAD2  
Affinity Capture-MS Homo sapiens
73 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 FSCN1 6624
Affinity Capture-MS Homo sapiens
75 YWHAQ 10971
Reconstituted Complex Homo sapiens
76 RFC4 5984
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
77 Pcna 18538
Affinity Capture-MS Mus musculus
78 RPS6 6194
Proximity Label-MS Homo sapiens
79 HECTD1 25831
Affinity Capture-MS Homo sapiens
80 LHX4  
Proximity Label-MS Homo sapiens
81 PRIM2 5558
Co-fractionation Homo sapiens
82 MAGEB2 4113
Affinity Capture-MS Homo sapiens
83 CEBPA  
Protein-peptide Homo sapiens
84 DAXX  
Affinity Capture-MS Homo sapiens
85 BRCA1 672
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
86 ZBTB44  
Affinity Capture-MS Homo sapiens
87 CD3EAP  
Proximity Label-MS Homo sapiens
88 SSBP3  
Two-hybrid Homo sapiens
89 Brwd3  
Affinity Capture-MS Mus musculus
90 FGFBP1 9982
Affinity Capture-MS Homo sapiens
91 CCNA2 890
Co-fractionation Homo sapiens
92 RAD17  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
93 HIST1H3A 8350
Proximity Label-MS Homo sapiens
94 MUS81  
Synthetic Growth Defect Homo sapiens
95 WDR18 57418
Co-fractionation Homo sapiens
96 MSH2 4436
Co-purification Homo sapiens
Co-fractionation Homo sapiens
97 SIRT7  
Affinity Capture-MS Homo sapiens
98 POLD3  
Co-fractionation Homo sapiens
99 ZSCAN1  
Two-hybrid Homo sapiens
100 RFC2 5982
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
101 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
102 SKP2  
Affinity Capture-MS Homo sapiens
103 NR3C1 2908
Proximity Label-MS Homo sapiens
104 BTF3 689
Affinity Capture-MS Homo sapiens
105 ATM 472
Co-purification Homo sapiens
106 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
107 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
108 ZC3H10  
Affinity Capture-MS Homo sapiens
109 CDK2 1017
Co-fractionation Homo sapiens
110 PCBP1 5093
Affinity Capture-MS Homo sapiens
111 PARP1 142
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
112 PSPC1 55269
Affinity Capture-MS Homo sapiens
113 RPA3 6119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
114 EMC2 9694
Affinity Capture-MS Homo sapiens
115 TOP1 7150
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
116 CDC45  
Co-fractionation Homo sapiens
117 ETV3  
Proximity Label-MS Homo sapiens
118 SAP25  
Affinity Capture-MS Homo sapiens
119 DDRGK1 65992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 CYB5R3 1727
Cross-Linking-MS (XL-MS) Homo sapiens
121 Kctd5  
Affinity Capture-MS Mus musculus
122 Rpl35 66489
Affinity Capture-MS Mus musculus
123 CCND1 595
Affinity Capture-Western Homo sapiens
124 LMNA 4000
Proximity Label-MS Homo sapiens
125 ZC3H3  
Affinity Capture-MS Homo sapiens
126 BRD3 8019
Affinity Capture-MS Homo sapiens
127 XRCC5 7520
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
128 RPS16 6217
Affinity Capture-MS Homo sapiens
129 RPL13 6137
Affinity Capture-MS Homo sapiens
130 RELA 5970
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
131 SURF6  
Affinity Capture-MS Homo sapiens
132 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
133 COPZ1 22818
Affinity Capture-MS Homo sapiens
134 SMC1A 8243
Co-fractionation Homo sapiens
135 LHX2  
Proximity Label-MS Homo sapiens
136 HDGF 3068
Affinity Capture-MS Homo sapiens
137 TP53 7157
Affinity Capture-Western Homo sapiens
138 SSRP1 6749
Affinity Capture-MS Homo sapiens
139 USP36  
Affinity Capture-MS Homo sapiens
140 RPA1 6117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
141 POLE3  
Co-fractionation Homo sapiens
142 PRIM1  
Co-fractionation Homo sapiens
143 MLH1 4292
Co-purification Homo sapiens
144 HDAC1 3065
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
145 NIFK 84365
Affinity Capture-MS Homo sapiens
146 WIF1 11197
Affinity Capture-MS Homo sapiens
147 RAD50 10111
Co-purification Homo sapiens
Co-fractionation Homo sapiens
148 MYOC 4653
Two-hybrid Homo sapiens
149 PCNA 5111
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
150 NUP50 10762
Proximity Label-MS Homo sapiens
151 NEIL1  
Affinity Capture-MS Homo sapiens
152 CDK4 1019
Affinity Capture-Western Homo sapiens
153 IBTK 25998
Affinity Capture-MS Homo sapiens
154 Rrbp1  
Affinity Capture-MS Mus musculus
155 CHTF18 63922
Co-fractionation Homo sapiens
156 GLYR1 84656
Co-fractionation Homo sapiens
157 XRCC6 2547
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
158 HNRNPU 3192
Affinity Capture-MS Homo sapiens
159 C9orf72  
Affinity Capture-MS Homo sapiens
160 RC3H1 149041
Affinity Capture-MS Homo sapiens
161 NUCKS1 64710
Affinity Capture-MS Homo sapiens
162 POLD2 5425
Co-fractionation Homo sapiens
163 NOTCH1 4851
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RFC1 is involved
PathwayEvidenceSource
Base Excision Repair TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Chromosome Maintenance TAS Reactome
DNA Damage Bypass TAS Reactome
DNA Double-Strand Break Repair TAS Reactome
DNA Repair TAS Reactome
DNA Replication TAS Reactome
DNA strand elongation TAS Reactome
Dual Incision in GG-NER TAS Reactome
Dual incision in TC-NER TAS Reactome
Extension of Telomeres TAS Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER TAS Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Global Genome Nucleotide Excision Repair (GG-NER) TAS Reactome
HDR through Homologous Recombination (HRR) TAS Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) TAS Reactome
Homology Directed Repair TAS Reactome
Lagging Strand Synthesis TAS Reactome
Leading Strand Synthesis TAS Reactome
Nucleotide Excision Repair TAS Reactome
PCNA-Dependent Long Patch Base Excision Repair TAS Reactome
Polymerase switching TAS Reactome
Polymerase switching on the C-strand of the telomere TAS Reactome
Recognition of DNA damage by PCNA-containing replication complex TAS Reactome
Resolution of Abasic Sites (AP sites) TAS Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway TAS Reactome
S Phase TAS Reactome
Synthesis of DNA TAS Reactome
Telomere C-strand (Lagging Strand) Synthesis TAS Reactome
Telomere Maintenance TAS Reactome
Termination of translesion DNA synthesis TAS Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Translesion Synthesis by POLH TAS Reactome
Translesion synthesis by POLI TAS Reactome
Translesion synthesis by POLK TAS Reactome
Translesion synthesis by REV1 TAS Reactome
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template TAS Reactome





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