Gene description for NUP50
Gene name nucleoporin 50kDa
Gene symbol NUP50
Other names/aliases NPAP60
NPAP60L
Species Homo sapiens
 Database cross references - NUP50
ExoCarta ExoCarta_10762
Vesiclepedia VP_10762
Entrez Gene 10762
HGNC 8065
MIM 604646
UniProt Q9UKX7  
 NUP50 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NUP50
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    neural tube formation GO:0001841 IEA
    protein import into nucleus GO:0006606 IBA
    nucleocytoplasmic transport GO:0006913 NAS
    mRNA transport GO:0051028 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear pore GO:0005643 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified NUP50 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NUP50
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MORC2  
Proximity Label-MS Homo sapiens
2 RNGTT 8732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SLFN11 91607
Proximity Label-MS Homo sapiens
4 CDCA2 157313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ZNF292  
Proximity Label-MS Homo sapiens
6 PRKAA1 5562
Co-fractionation Homo sapiens
7 AP2B1 163
Co-fractionation Homo sapiens
8 BAZ2A  
Proximity Label-MS Homo sapiens
9 SUPT6H 6830
Proximity Label-MS Homo sapiens
10 PPP1R3A  
Affinity Capture-MS Homo sapiens
11 ARHGEF19  
Affinity Capture-MS Homo sapiens
12 GPATCH4 54865
Proximity Label-MS Homo sapiens
13 COIL  
Proximity Label-MS Homo sapiens
14 PMS1  
Proximity Label-MS Homo sapiens
15 KPNA1 3836
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LDHB 3945
Co-fractionation Homo sapiens
17 SRPK2 6733
Biochemical Activity Homo sapiens
18 FAM192A  
Proximity Label-MS Homo sapiens
19 SSB 6741
Proximity Label-MS Homo sapiens
20 GLB1 2720
Co-fractionation Homo sapiens
21 KPNB1 3837
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ZC3H14  
Proximity Label-MS Homo sapiens
23 ACTC1 70
Proximity Label-MS Homo sapiens
24 SENP1  
Proximity Label-MS Homo sapiens
25 BRD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SPEN 23013
Proximity Label-MS Homo sapiens
27 ESF1 51575
Proximity Label-MS Homo sapiens
28 TAF3 83860
Proximity Label-MS Homo sapiens
29 C9orf78 51759
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
30 ZFR 51663
Proximity Label-MS Homo sapiens
31 CSE1L 1434
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
32 SPOPL  
Affinity Capture-MS Homo sapiens
33 KPNA2 3838
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CCDC94  
Proximity Label-MS Homo sapiens
35 ARID3B  
Proximity Label-MS Homo sapiens
36 SBNO1 55206
Proximity Label-MS Homo sapiens
37 SMARCD2 6603
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RFX1  
Proximity Label-MS Homo sapiens
39 APP 351
Reconstituted Complex Homo sapiens
40 WDR36 134430
Proximity Label-MS Homo sapiens
41 EPB41L1 2036
Co-fractionation Homo sapiens
42 ZMAT2  
Proximity Label-MS Homo sapiens
43 APEX1 328
Affinity Capture-RNA Homo sapiens
44 KIAA1429 25962
Affinity Capture-MS Homo sapiens
45 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
46 ZSCAN21  
Proximity Label-MS Homo sapiens
47 MED1 5469
Proximity Label-MS Homo sapiens
48 NCOR1  
Proximity Label-MS Homo sapiens
49 RDX 5962
Co-fractionation Homo sapiens
50 NISCH 11188
Affinity Capture-MS Homo sapiens
51 BCL7B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DGCR14  
Proximity Label-MS Homo sapiens
53 WRNIP1 56897
Proximity Label-MS Homo sapiens
54 HELZ 9931
Affinity Capture-MS Homo sapiens
55 KPNA3 3839
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 AP2A1 160
Co-fractionation Homo sapiens
57 NUP153 9972
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 KIF5B 3799
Co-fractionation Homo sapiens
59 NR2F2  
Co-fractionation Homo sapiens
60 CDK12 51755
Proximity Label-MS Homo sapiens
61 TOR1AIP1 26092
Co-fractionation Homo sapiens
62 CUX1 1523
Proximity Label-MS Homo sapiens
63 NPM1 4869
Affinity Capture-Western Homo sapiens
64 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 KLHL7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PFDN4 5203
Co-fractionation Homo sapiens
67 WIZ 58525
Proximity Label-MS Homo sapiens
68 NARS 4677
Co-fractionation Homo sapiens
69 SIRT1  
Proximity Label-MS Homo sapiens
70 BOD1L1 259282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
72 ZNF451 26036
Proximity Label-MS Homo sapiens
73 ZNF512B  
Proximity Label-MS Homo sapiens
74 WDR3  
Proximity Label-MS Homo sapiens
75 PEPD 5184
Co-fractionation Homo sapiens
76 BCOR  
Proximity Label-MS Homo sapiens
77 YEATS2  
Proximity Label-MS Homo sapiens
78 ZC3H4 23211
Proximity Label-MS Homo sapiens
79 BTAF1 9044
Proximity Label-MS Homo sapiens
80 CDC73  
Proximity Label-MS Homo sapiens
81 NSD1  
Proximity Label-MS Homo sapiens
82 MECP2 4204
Proximity Label-MS Homo sapiens
83 SLC4A1AP  
Proximity Label-MS Homo sapiens
84 MYCN  
Affinity Capture-MS Homo sapiens
85 BUD31 8896
Proximity Label-MS Homo sapiens
86 TCERG1 10915
Proximity Label-MS Homo sapiens
87 TFIP11  
Proximity Label-MS Homo sapiens
88 KIF14 9928
Affinity Capture-MS Homo sapiens
89 EMC9  
Affinity Capture-MS Homo sapiens
90 PSME3 10197
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
91 NUP155 9631
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
92 GARS 2617
Co-fractionation Homo sapiens
93 HSPBP1 23640
Co-fractionation Homo sapiens
94 CXXC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 KIFC1 3833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 PHF10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 SMARCD1 6602
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 C10orf12  
Proximity Label-MS Homo sapiens
99 BRIP1  
Proximity Label-MS Homo sapiens
100 MCM3AP  
Proximity Label-MS Homo sapiens
101 Rcc1  
Affinity Capture-MS Mus musculus
102 SAP130  
Proximity Label-MS Homo sapiens
103 EMD 2010
Proximity Label-MS Homo sapiens
104 WDR70 55100
Proximity Label-MS Homo sapiens
105 TNPO1 3842
Affinity Capture-MS Homo sapiens
106 RNF169  
Proximity Label-MS Homo sapiens
107 RBM6 10180
Proximity Label-MS Homo sapiens
108 SUPT5H 6829
Proximity Label-MS Homo sapiens
109 PAN2  
Affinity Capture-MS Homo sapiens
110 BLM 641
Proximity Label-MS Homo sapiens
111 MAPRE1 22919
Affinity Capture-MS Homo sapiens
112 XPO5 57510
Proximity Label-MS Homo sapiens
113 NOL11  
Proximity Label-MS Homo sapiens
114 NELFCD 51497
Proximity Label-MS Homo sapiens
115 KPNA4 3840
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 SRRM2 23524
Proximity Label-MS Homo sapiens
117 ACACA 31
Co-fractionation Homo sapiens
118 CFDP1  
Proximity Label-MS Homo sapiens
119 PFDN2 5202
Co-fractionation Homo sapiens
120 RAN 5901
Affinity Capture-MS Homo sapiens
121 NTRK1 4914
Affinity Capture-MS Homo sapiens
122 DHX16 8449
Proximity Label-MS Homo sapiens
123 Mki67 17345
Affinity Capture-MS Mus musculus
124 DTX2 113878
Proximity Label-MS Homo sapiens
125 NACA 4666
Cross-Linking-MS (XL-MS) Homo sapiens
126 TSC22D1 8848
Co-fractionation Homo sapiens
127 KIAA1143  
Proximity Label-MS Homo sapiens
128 FLNB 2317
Co-fractionation Homo sapiens
129 WDR43 23160
Proximity Label-MS Homo sapiens
130 RPA4  
Proximity Label-MS Homo sapiens
131 UTP14A 10813
Proximity Label-MS Homo sapiens
132 GPKOW  
Proximity Label-MS Homo sapiens
133 CCDC22 28952
Co-fractionation Homo sapiens
134 SOWAHA  
Affinity Capture-MS Homo sapiens
135 WHSC1L1  
Proximity Label-MS Homo sapiens
136 CHD7  
Proximity Label-MS Homo sapiens
137 Ube2i  
Affinity Capture-MS Mus musculus
138 SON 6651
Proximity Label-MS Homo sapiens
139 KPNA5 3841
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 AHCTF1 25909
Proximity Label-MS Homo sapiens
141 KIAA1033 23325
Co-fractionation Homo sapiens
142 PPM1G 5496
Proximity Label-MS Homo sapiens
143 CEBPA  
Protein-peptide Homo sapiens
144 USP28 57646
Proximity Label-MS Homo sapiens
145 TTLL12 23170
Proximity Label-MS Homo sapiens
146 OGT 8473
Reconstituted Complex Homo sapiens
147 SMARCC2 6601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 GTF2A1 2957
Proximity Label-MS Homo sapiens
149 DHX8 1659
Proximity Label-MS Homo sapiens
150 STK10 6793
Co-fractionation Homo sapiens
151 HNRNPDL 9987
Co-fractionation Homo sapiens
152 TTI1 9675
Co-fractionation Homo sapiens
153 RBBP6 5930
Proximity Label-MS Homo sapiens
154 SLX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 ANO5  
Proximity Label-MS Homo sapiens
156 KHSRP 8570
Co-fractionation Homo sapiens
157 KMT2A  
Proximity Label-MS Homo sapiens
158 Nup98  
Affinity Capture-MS Mus musculus
159 RAB7A 7879
Proximity Label-MS Homo sapiens
160 TP53BP1 7158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
161 NOL8  
Proximity Label-MS Homo sapiens
162 XPO1 7514
Affinity Capture-MS Homo sapiens
163 LMNB1 4001
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 EIF4G1 1981
Co-fractionation Homo sapiens
165 UBAP2 55833
Co-fractionation Homo sapiens
166 DACH1  
Proximity Label-MS Homo sapiens
167 Kifc1  
Affinity Capture-MS Mus musculus
168 STXBP1 6812
Co-fractionation Homo sapiens
169 HTATSF1 27336
Proximity Label-MS Homo sapiens
170 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
171 PRCC  
Proximity Label-MS Homo sapiens
172 RPRD2  
Proximity Label-MS Homo sapiens
173 PHIP 55023
Proximity Label-MS Homo sapiens
174 CUL3 8452
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
175 XAB2 56949
Proximity Label-MS Homo sapiens
176 C1orf52  
Proximity Label-MS Homo sapiens
177 ZNF830  
Proximity Label-MS Homo sapiens
178 ZEB1  
Proximity Label-MS Homo sapiens
179 POM121  
Proximity Label-MS Homo sapiens
180 NUP43 348995
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
181 NACC1 112939
Proximity Label-MS Homo sapiens
182 NUP214 8021
Affinity Capture-MS Homo sapiens
183 MYC  
Affinity Capture-MS Homo sapiens
184 RBM33  
Proximity Label-MS Homo sapiens
185 SRFBP1  
Proximity Label-MS Homo sapiens
186 GTF2E1 2960
Proximity Label-MS Homo sapiens
187 KIAA0430  
Affinity Capture-MS Homo sapiens
188 RFC1 5981
Proximity Label-MS Homo sapiens
189 NCBP2 22916
Affinity Capture-MS Homo sapiens
190 KDM3B  
Proximity Label-MS Homo sapiens
191 SUGP1  
Proximity Label-MS Homo sapiens
192 PMPCB 9512
Co-fractionation Homo sapiens
193 MRGBP  
Affinity Capture-MS Homo sapiens
194 NCAPH 23397
Affinity Capture-MS Homo sapiens
195 DDX42 11325
Proximity Label-MS Homo sapiens
196 IMPDH2 3615
Co-fractionation Homo sapiens
197 MFAP1  
Proximity Label-MS Homo sapiens
198 Nup107  
Affinity Capture-MS Mus musculus
199 RPA3 6119
Proximity Label-MS Homo sapiens
200 SEC31A 22872
Co-fractionation Homo sapiens
201 PPWD1 23398
Proximity Label-MS Homo sapiens
202 GMFB 2764
Co-fractionation Homo sapiens
203 RBM27  
Proximity Label-MS Homo sapiens
204 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
205 RAI1 10743
Proximity Label-MS Homo sapiens
206 SART3 9733
Co-fractionation Homo sapiens
207 SGTA 6449
Proximity Label-MS Homo sapiens
208 COPA 1314
Proximity Label-MS Homo sapiens
209 CDKN1B 1027
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
210 KDM3A  
Proximity Label-MS Homo sapiens
211 LEMD3  
Proximity Label-MS Homo sapiens
212 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
213 MYSM1  
Proximity Label-MS Homo sapiens
214 RANBP1 5902
Co-fractionation Homo sapiens
215 TAF4 6874
Affinity Capture-MS Homo sapiens
216 RAE1 8480
Proximity Label-MS Homo sapiens
217 UBXN7 26043
Proximity Label-MS Homo sapiens
218 PAIP1 10605
Co-fractionation Homo sapiens
219 SBDS 51119
Co-fractionation Homo sapiens
220 RPL13 6137
Cross-Linking-MS (XL-MS) Homo sapiens
221 IKBKAP 8518
Co-fractionation Homo sapiens
222 TAF15 8148
Proximity Label-MS Homo sapiens
223 FNBP4  
Proximity Label-MS Homo sapiens
224 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
225 FBXW11  
Affinity Capture-MS Homo sapiens
226 CLSPN  
Proximity Label-MS Homo sapiens
227 SPOP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
228 SMARCD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
229 ZNF384  
Proximity Label-MS Homo sapiens
230 ARID2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
231 NUP133 55746
Proximity Label-MS Homo sapiens
232 NSF 4905
Co-fractionation Homo sapiens
233 FBXL6  
Proximity Label-MS Homo sapiens
234 PLEC 5339
Co-fractionation Homo sapiens
235 TRIM33 51592
Proximity Label-MS Homo sapiens
236 RGPD8  
Affinity Capture-MS Homo sapiens
237