Gene description for TAF15
Gene name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Gene symbol TAF15
Other names/aliases Npl3
RBP56
TAF2N
TAFII68
Species Homo sapiens
 Database cross references - TAF15
ExoCarta ExoCarta_8148
Entrez Gene 8148
HGNC 11547
MIM 601574
UniProt Q92804  
 TAF15 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TAF15
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    DNA binding GO:0003677 IEA
    zinc ion binding GO:0008270 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    positive regulation of transcription, DNA-templated GO:0045893 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified TAF15 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for TAF15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TAF5  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 POLR2C 5432
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 TBP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 POLR2E 5434
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 TAF7  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 TAF11  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 SF1 7536
Reconstituted Complex Homo sapiens
8 PRMT1 3276
Biochemical Activity Homo sapiens
9 SAFB  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 TAF13  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 POLR2G  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TAF15 is involved
No pathways found





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