Gene description for CUL2
Gene name cullin 2
Gene symbol CUL2
Other names/aliases -
Species Homo sapiens
 Database cross references - CUL2
ExoCarta ExoCarta_8453
Entrez Gene 8453
HGNC 2552
MIM 603135
UniProt Q13617  
 CUL2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CUL2
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IDA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    negative regulation of cell proliferation GO:0008285 TAS
    cell cycle arrest GO:0007050 TAS
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    cellular response to hypoxia GO:0071456 TAS
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    viral process GO:0016032 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    Cul2-RING ubiquitin ligase complex GO:0031462 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CUL2 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CUL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAB39 51719
Affinity Capture-MS Homo sapiens
2 RNF7  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 STOM 2040
Affinity Capture-MS Homo sapiens
4 P4HB 5034
Affinity Capture-MS Homo sapiens
5 TFE3  
Affinity Capture-MS Homo sapiens
6 PFKFB3 5209
Affinity Capture-MS Homo sapiens
7 LOC441246  
Affinity Capture-MS Homo sapiens
8 RNMTL1  
Affinity Capture-MS Homo sapiens
9 RBX1 9978
Affinity Capture-MS Homo sapiens
10 KCTD5  
Affinity Capture-MS Homo sapiens
11 TIMM13  
Affinity Capture-MS Homo sapiens
12 VHL  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 CMBL 134147
Affinity Capture-MS Homo sapiens
15 DLG3 1741
Affinity Capture-MS Homo sapiens
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