Gene description for DLG3
Gene name discs, large homolog 3 (Drosophila)
Gene symbol DLG3
Other names/aliases MRX
MRX90
NEDLG
PPP1R82
SAP102
XLMR
Species Homo sapiens
 Database cross references - DLG3
ExoCarta ExoCarta_1741
Vesiclepedia VP_1741
Entrez Gene 1741
HGNC 2902
MIM 300189
UniProt Q92796  
 DLG3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DLG3
Molecular Function
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IDA
    protein kinase binding GO:0019901 IBA
    phosphatase binding GO:0019902 IDA
    ubiquitin protein ligase binding GO:0031625 IEA
    ionotropic glutamate receptor binding GO:0035255 IBA
Biological Process
    establishment of planar polarity GO:0001736 IEA
    chemical synaptic transmission GO:0007268 IBA
    nervous system development GO:0007399 IBA
    negative regulation of cell population proliferation GO:0008285 NAS
    protein localization to synapse GO:0035418 IBA
    receptor clustering GO:0043113 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 NAS
    receptor localization to synapse GO:0097120 IBA
    cell-cell adhesion GO:0098609 IBA
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IBA
Subcellular Localization
    extracellular space GO:0005615 HDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 NAS
    bicellular tight junction GO:0005923 IEA
    basolateral plasma membrane GO:0016323 IBA
    neuromuscular junction GO:0031594 IBA
    AMPA glutamate receptor complex GO:0032281 IEA
    neuron projection GO:0043005 IBA
    postsynaptic density membrane GO:0098839 IBA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified DLG3 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DLG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT34  
Affinity Capture-MS Homo sapiens
2 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
3 ARHGAP17 55114
Affinity Capture-MS Homo sapiens
4 KRT85  
Affinity Capture-MS Homo sapiens
5 EIF1AD 84285
Affinity Capture-MS Homo sapiens
6 NBEA  
Affinity Capture-MS Homo sapiens
7 GRIK2  
Affinity Capture-Western Homo sapiens
8 RALGAPA1 253959
Affinity Capture-MS Homo sapiens
9 EXOC7 23265
Reconstituted Complex Homo sapiens
10 DLGAP2  
Affinity Capture-MS Homo sapiens
11 KCNA4  
Affinity Capture-MS Homo sapiens
12 TARBP1 6894
Affinity Capture-MS Homo sapiens
13 EXOC3 11336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 PRNP 5621
Affinity Capture-MS Homo sapiens
15 HIST1H2BC 8347
Affinity Capture-MS Homo sapiens
16 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
17 RFWD2  
Affinity Capture-Western Homo sapiens
18 RACGAP1 29127
Affinity Capture-MS Homo sapiens
19 KRT35 3886
Affinity Capture-MS Homo sapiens
20 DLGAP1  
Two-hybrid Homo sapiens
21 MRPL18 29074
Affinity Capture-MS Homo sapiens
22 CAMK2A 815
Affinity Capture-MS Homo sapiens
23 WDR91 29062
Affinity Capture-MS Homo sapiens
24 NLGN3  
Two-hybrid Homo sapiens
25 ANXA1 301
Affinity Capture-MS Homo sapiens
26 APC  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
27 GUCY1A2  
Affinity Capture-Western Homo sapiens
28 GDA 9615
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 CNPY2 10330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SS18L2  
Affinity Capture-MS Homo sapiens
31 ACAD8 27034
Affinity Capture-MS Homo sapiens
32 ERBB4 2066
Two-hybrid Homo sapiens
33 RPS6KA4 8986
Co-fractionation Homo sapiens
34 GRIN2C 2905
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
35 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
36 CALM1 801
Affinity Capture-MS Homo sapiens
37 RB1CC1 9821
Affinity Capture-MS Homo sapiens
38 DLG4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
39 SYNGAP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
40 DLGAP4 22839
Affinity Capture-MS Homo sapiens
41 C11orf52 91894
Proximity Label-MS Homo sapiens
42 RALGAPA2  
Affinity Capture-MS Homo sapiens
43 KRT82 3888
Affinity Capture-MS Homo sapiens
44 ZNF232  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 EXOC4 60412
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
46 RALGAPB 57148
Affinity Capture-MS Homo sapiens
47 S100A3  
Affinity Capture-MS Homo sapiens
48 MRPL22  
Affinity Capture-MS Homo sapiens
49 CUL2 8453
Affinity Capture-MS Homo sapiens
50 DLG2 1740
Affinity Capture-MS Homo sapiens
51 Sstr2  
Two-hybrid Rattus norvegicus
52 TFAM 7019
Cross-Linking-MS (XL-MS) Homo sapiens
53 GRIN2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 GRIK5  
Affinity Capture-Western Homo sapiens
55 OGT 8473
Reconstituted Complex Homo sapiens
56 KLHDC3  
Affinity Capture-MS Homo sapiens
57 ATP2B2 491
Affinity Capture-Western Homo sapiens
58 LRP2 4036
Two-hybrid Homo sapiens
59 PTK2B 2185
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
60 COBLL1 22837
Affinity Capture-MS Homo sapiens
61 NEDD4L 23327
Affinity Capture-MS Homo sapiens
62 RHBDL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 EPHA2 1969
Proximity Label-MS Homo sapiens
64 NLGN1  
Two-hybrid Homo sapiens
65 ZFP91 80829
Affinity Capture-MS Homo sapiens
66 KRAS 3845
Proximity Label-MS Homo sapiens
67 RHOB 388
Proximity Label-MS Homo sapiens
68 KHDRBS1 10657
Protein-peptide Homo sapiens
69 GRIN2A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
70 AP3B1 8546
Affinity Capture-MS Homo sapiens
71 ATP2B4 493
Affinity Capture-Western Homo sapiens
72 SEMA4C 54910
Two-hybrid Homo sapiens
73 MRPS26 64949
Affinity Capture-MS Homo sapiens
74 KRT31 3881
Affinity Capture-MS Homo sapiens
75 KCNJ12 3768
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
76 CRIPT 9419
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
77 TEAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 NLGN2 57555
Two-hybrid Homo sapiens
79 KCTD2  
Affinity Capture-MS Homo sapiens
80 ARFGAP1 55738
Co-fractionation Homo sapiens
81 DLG1 1739
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DLG3 is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor TAS Reactome
Activation of kainate receptors upon glutamate binding TAS Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of NMDA receptors and postsynaptic events TAS Reactome
Assembly and cell surface presentation of NMDA receptors IEA Reactome
Axon guidance IEA Reactome
CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling IEA Reactome
Developmental Biology IEA Reactome
Ionotropic activity of kainate receptors TAS Reactome
L1CAM interactions IEA Reactome
Long-term potentiation IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative regulation of NMDA receptor-mediated neuronal transmission IEA Reactome
Nervous system development IEA Reactome
Neurexins and neuroligins IEA Reactome
Neurexins and neuroligins TAS Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
NrCAM interactions IEA Reactome
Post NMDA receptor activation events IEA Reactome
Protein-protein interactions at synapses IEA Reactome
Protein-protein interactions at synapses TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Ras activation upon Ca2+ influx through NMDA receptor IEA Reactome
Signal Transduction TAS Reactome
Synaptic adhesion-like molecules TAS Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome
Unblocking of NMDA receptors, glutamate binding and activation TAS Reactome
Unblocking of NMDA receptors, glutamate binding and activation IEA Reactome





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