Gene description for DLG3
Gene name discs, large homolog 3 (Drosophila)
Gene symbol DLG3
Other names/aliases MRX
MRX90
NEDLG
PPP1R82
SAP102
XLMR
Species Homo sapiens
 Database cross references - DLG3
ExoCarta ExoCarta_1741
Entrez Gene 1741
HGNC 2902
MIM 300189
UniProt Q92796  
 DLG3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DLG3
Molecular Function
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IDA
    protein C-terminus binding GO:0008022 IEA
    guanylate kinase activity GO:0004385 IBA
    ionotropic glutamate receptor binding GO:0035255 IBA
    PDZ domain binding GO:0030165 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    protein phosphatase binding GO:0019903 IEA
    phosphatase binding GO:0019902 IDA
Biological Process
    establishment of planar polarity GO:0001736 IEA
    receptor clustering GO:0043113 IBA
    nucleotide phosphorylation GO:0046939 IBA
    negative regulation of phosphatase activity GO:0010923 IDA
    nervous system development GO:0007399 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    negative regulation of cell proliferation GO:0008285 NAS
    receptor localization to synapse GO:0097120 IBA
    axon guidance GO:0007411 TAS
    synaptic transmission GO:0007268 TAS
Subcellular Localization
    ionotropic glutamate receptor complex GO:0008328 IBA
    basolateral plasma membrane GO:0016323 IBA
    postsynaptic density GO:0014069 IBA
    plasma membrane GO:0005886 TAS
    dendritic shaft GO:0043198 IEA
    cytoplasm GO:0005737 IEA
    neuronal cell body GO:0043025 IEA
    growth cone GO:0030426 IEA
    extracellular space GO:0005615 IDA
    postsynaptic membrane GO:0045211 IBA
    bicellular tight junction GO:0005923 IEA
    alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex GO:0032281 IEA
 Experiment description of studies that identified DLG3 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DLG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EXOC7 23265
Reconstituted Complex Homo sapiens
2 GDA 9615
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 CUL2 8453
Affinity Capture-MS Homo sapiens
4 EXOC3 11336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 KRTHA5  
Affinity Capture-MS Homo sapiens
6 DLGAP1  
Two-hybrid Homo sapiens
7 KLHDC3  
Affinity Capture-MS Homo sapiens
8 NLGN3  
Two-hybrid Homo sapiens
9 ANXA1 301
Affinity Capture-MS Homo sapiens
10 APC  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 NLGN2  
Two-hybrid Homo sapiens
12 HIST1H2BC 8347
Affinity Capture-MS Homo sapiens
13 ERBB4 2066
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 KRTHB2  
Affinity Capture-MS Homo sapiens
15 S100A3  
Affinity Capture-MS Homo sapiens
16 DLG4  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
17 SYNGAP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 EXOC4 60412
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 LIN7A 8825
Invitro Homo sapiens
20 GRIN2B  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 LRP2 4036
Two-hybrid Homo sapiens
22 PTK2B 2185
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 KRT31 3881
Affinity Capture-MS Homo sapiens
24 NLGN1  
Two-hybrid Homo sapiens
25 KRTHA4  
Affinity Capture-MS Homo sapiens
26 GRIN2A  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
27 CAMK2A  
Affinity Capture-MS Homo sapiens
28 ATP2B4 493
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
29 SEMA4C 54910
Two-hybrid Homo sapiens
30 GRIN2C  
Invitro Homo sapiens
Invivo Homo sapiens
31 KCNJ12 3768
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 CRIPT 9419
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
33 GUCY1A2  
Invitro Homo sapiens
34 KRTHB5  
Affinity Capture-MS Homo sapiens
35 DLG1 1739
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which DLG3 is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor TAS Reactome
NrCAM interactions TAS Reactome





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