Gene description for ACAD8
Gene name acyl-CoA dehydrogenase family, member 8
Gene symbol ACAD8
Other names/aliases ACAD-8
ARC42
Species Homo sapiens
 Database cross references - ACAD8
ExoCarta ExoCarta_27034
Entrez Gene 27034
HGNC 87
MIM 604773
UniProt Q9UKU7  
 ACAD8 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ACAD8
Molecular Function
    flavin adenine dinucleotide binding GO:0050660 IEA
    acyl-CoA dehydrogenase activity GO:0003995 EXP
Biological Process
    valine catabolic process GO:0006574 IEA
    small molecule metabolic process GO:0044281 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    oxidation-reduction process GO:0055114 IEA
    lipid metabolic process GO:0006629 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    branched-chain amino acid catabolic process GO:0009083 TAS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ACAD8 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ACAD8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ACAD8 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism TAS Reactome





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