Gene description for ACAD8
Gene name acyl-CoA dehydrogenase family, member 8
Gene symbol ACAD8
Other names/aliases ACAD-8
ARC42
Species Homo sapiens
 Database cross references - ACAD8
ExoCarta ExoCarta_27034
Vesiclepedia VP_27034
Entrez Gene 27034
HGNC 87
MIM 604773
UniProt Q9UKU7  
 ACAD8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ACAD8
Molecular Function
    short-chain 2-methyl fatty acyl-CoA dehydrogenase activity GO:0003853 IEA
    acyl-CoA dehydrogenase activity GO:0003995 TAS
    protein binding GO:0005515 IPI
    flavin adenine dinucleotide binding GO:0050660 IEA
Biological Process
    valine catabolic process GO:0006574 IEA
    lipid metabolic process GO:0006629 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ACAD8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ACAD8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FCGR1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RHOU 58480
Reconstituted Complex Homo sapiens
3 POLM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PTPN22  
Affinity Capture-MS Homo sapiens
5 DLG3 1741
Affinity Capture-MS Homo sapiens
6 Nek2  
Affinity Capture-MS Mus musculus
7 CYP2C18  
Affinity Capture-MS Homo sapiens
8 TRUB2  
Affinity Capture-MS Homo sapiens
9 SRGAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MTMR14 64419
Affinity Capture-MS Homo sapiens
11 FAM161B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CD79A  
Affinity Capture-MS Homo sapiens
13 LDHD  
Affinity Capture-MS Homo sapiens
14 PPP1CC 5501
Two-hybrid Homo sapiens
15 ACADSB 36
Co-fractionation Homo sapiens
16 Tgfbr1 21812
Affinity Capture-MS Mus musculus
17 VSTM2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ABHD11 83451
Affinity Capture-MS Homo sapiens
19 PTPN21 11099
Affinity Capture-MS Homo sapiens
20 CKAP5 9793
Affinity Capture-MS Homo sapiens
21 GP5 2814
Affinity Capture-MS Homo sapiens
22 VPS37D 155382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GMIP  
Affinity Capture-MS Homo sapiens
24 TNS2 23371
Affinity Capture-MS Homo sapiens
25 DUSP14 11072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HSPD1 3329
Affinity Capture-MS Homo sapiens
27 OLIG1  
Affinity Capture-MS Homo sapiens
28 IPO8 10526
Affinity Capture-MS Homo sapiens
29 CUL4A 8451
Affinity Capture-MS Homo sapiens
30 PTPN14 5784
Affinity Capture-MS Homo sapiens
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