Gene description for RHOU
Gene name ras homolog family member U
Gene symbol RHOU
Other names/aliases ARHU
CDC42L1
G28K
WRCH1
hG28K
Species Homo sapiens
 Database cross references - RHOU
ExoCarta ExoCarta_58480
Vesiclepedia VP_58480
Entrez Gene 58480
HGNC 17794
MIM 606366
UniProt Q7L0Q8  
 RHOU identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RHOU
Molecular Function
    GTPase activity GO:0003924 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    protein kinase binding GO:0019901 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IEA
    endocytosis GO:0006897 IBA
    cytoskeleton organization GO:0007010 IMP
    actin filament organization GO:0007015 IBA
    regulation of cell shape GO:0008360 IMP
    Rac protein signal transduction GO:0016601 IEA
    establishment of cell polarity GO:0030010 IBA
    Cdc42 protein signal transduction GO:0032488 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    podosome GO:0002102 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IEA
    endosome membrane GO:0010008 TAS
    cell projection GO:0042995 IEA
 Experiment description of studies that identified RHOU in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RHOU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ACAD8 27034
Reconstituted Complex Homo sapiens
3 ARHGEF25 115557
Reconstituted Complex Homo sapiens
4 RPS17P16  
Reconstituted Complex Homo sapiens
5 KLRC3  
Reconstituted Complex Homo sapiens
6 RPL23AP5  
Reconstituted Complex Homo sapiens
7 CHRNA4  
Reconstituted Complex Homo sapiens
8 POT1  
Reconstituted Complex Homo sapiens
9 SKIV2L 6499
Reconstituted Complex Homo sapiens
10 MARCKS 4082
Reconstituted Complex Homo sapiens
11 ERO1L 30001
Reconstituted Complex Homo sapiens
12 PSMA4 5685
Reconstituted Complex Homo sapiens
13 FKBP15 23307
Reconstituted Complex Homo sapiens
14 SIVA1  
Reconstituted Complex Homo sapiens
15 XPA  
Reconstituted Complex Homo sapiens
16 IFNGR1 3459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ZNF20  
Reconstituted Complex Homo sapiens
18 ANXA6 309
Reconstituted Complex Homo sapiens
19 VWA5A 4013
Reconstituted Complex Homo sapiens
20 UTP3 57050
Reconstituted Complex Homo sapiens
21 ARNTL  
Reconstituted Complex Homo sapiens
22 ATP5L 10632
Reconstituted Complex Homo sapiens
23 TCN2 6948
Reconstituted Complex Homo sapiens
24 MOS 4342
Reconstituted Complex Homo sapiens
25 S100A4 6275
Reconstituted Complex Homo sapiens
26 ERF  
Reconstituted Complex Homo sapiens
27 SSC4D  
Reconstituted Complex Homo sapiens
28 PAK4 10298
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
29 HIST1H2BM 8342
Reconstituted Complex Homo sapiens
30 LAYN 143903
Reconstituted Complex Homo sapiens
31 C8orf59  
Reconstituted Complex Homo sapiens
32 SYK 6850
Reconstituted Complex Homo sapiens
33 TEKT2  
Reconstituted Complex Homo sapiens
34 NR1H2  
Reconstituted Complex Homo sapiens
35 ZNF117  
Reconstituted Complex Homo sapiens
36 BNIPL  
Reconstituted Complex Homo sapiens
37 HINT3 135114
Reconstituted Complex Homo sapiens
38 PLEK 5341
Reconstituted Complex Homo sapiens
39 P4HTM  
Reconstituted Complex Homo sapiens
40 ATP6AP1 537
Reconstituted Complex Homo sapiens
41 CPNE3 8895
Reconstituted Complex Homo sapiens
42 CTCF  
Reconstituted Complex Homo sapiens
43 NXPH4  
Reconstituted Complex Homo sapiens
44 TRIM59  
Reconstituted Complex Homo sapiens
45 PAK1 5058
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
46 LYL1  
Reconstituted Complex Homo sapiens
47 CLEC7A  
Reconstituted Complex Homo sapiens
48 WNT6  
Reconstituted Complex Homo sapiens
49 MRAS 22808
Reconstituted Complex Homo sapiens
50 PDLIM3 27295
Reconstituted Complex Homo sapiens
51 ZNF177  
Reconstituted Complex Homo sapiens
52 JAM3 83700
Reconstituted Complex Homo sapiens
53 AP1S1 1174
Reconstituted Complex Homo sapiens
54 LMO7 4008
Reconstituted Complex Homo sapiens
55 ZNF84  
Reconstituted Complex Homo sapiens
56 GPRIN2  
Reconstituted Complex Homo sapiens
57 GLI3 2737
Reconstituted Complex Homo sapiens
58 ANXA4 307
Reconstituted Complex Homo sapiens
59 NRBF2  
Reconstituted Complex Homo sapiens
60 HCAR1  
Reconstituted Complex Homo sapiens
61 ANGPT4  
Reconstituted Complex Homo sapiens
62 PRPF18  
Reconstituted Complex Homo sapiens
63 ZKSCAN7  
Reconstituted Complex Homo sapiens
64 FAM110B  
Reconstituted Complex Homo sapiens
65 DNM2 1785
Reconstituted Complex Homo sapiens
66 RPS15A 6210
Reconstituted Complex Homo sapiens
67 KAAG1  
Reconstituted Complex Homo sapiens
68 DACT2  
Reconstituted Complex Homo sapiens
69 ID2  
Reconstituted Complex Homo sapiens
70 AS3MT 57412
Reconstituted Complex Homo sapiens
71 BRINP3  
Reconstituted Complex Homo sapiens
72 ZNF747  
Reconstituted Complex Homo sapiens
73 PCDHGA4  
Reconstituted Complex Homo sapiens
74 IP6K2  
Affinity Capture-MS Homo sapiens
75 BRF1  
Reconstituted Complex Homo sapiens
76 NCR1  
Reconstituted Complex Homo sapiens
77 ZNF569  
Reconstituted Complex Homo sapiens
78 KIAA1191  
Reconstituted Complex Homo sapiens
79 C14orf119  
Reconstituted Complex Homo sapiens
80 SLC26A1  
Reconstituted Complex Homo sapiens
81 TMEM255B  
Reconstituted Complex Homo sapiens
82 NPM3 10360
Reconstituted Complex Homo sapiens
83 DOK4 55715
Reconstituted Complex Homo sapiens
84 IFFO1  
Reconstituted Complex Homo sapiens
85 RASSF1  
Reconstituted Complex Homo sapiens
86 SEC14L4 284904
Reconstituted Complex Homo sapiens
87 TMEM169  
Reconstituted Complex Homo sapiens
88 MRPL15 29088
Reconstituted Complex Homo sapiens
89 RAD51D  
Reconstituted Complex Homo sapiens
90 TMEM106A  
Reconstituted Complex Homo sapiens
91 HSD17B13  
Reconstituted Complex Homo sapiens
92 PRB1  
Reconstituted Complex Homo sapiens
93 FCHO2 115548
Reconstituted Complex Homo sapiens
94 EMC2 9694
Reconstituted Complex Homo sapiens
95 CDKL4  
Reconstituted Complex Homo sapiens
96 NHP2L1 4809
Reconstituted Complex Homo sapiens
97 GALNTL5  
Reconstituted Complex Homo sapiens
98 QTRT1 81890
Reconstituted Complex Homo sapiens
99 CCDC37 348807
Reconstituted Complex Homo sapiens
100 ZFAND2B  
Reconstituted Complex Homo sapiens
101 LANCL2 55915
Reconstituted Complex Homo sapiens
102 ADM2  
Reconstituted Complex Homo sapiens
103 PIP5K1A 8394
Reconstituted Complex Homo sapiens
104 RALBP1 10928
Reconstituted Complex Homo sapiens
105 ALG13 79868
Reconstituted Complex Homo sapiens
106 USP37  
Reconstituted Complex Homo sapiens
107 STX16 8675
Reconstituted Complex Homo sapiens
108 ME3 10873
Reconstituted Complex Homo sapiens
109 LARS2 23395
Reconstituted Complex Homo sapiens
110 RAB40A  
Reconstituted Complex Homo sapiens
111 CUL5 8065
Reconstituted Complex Homo sapiens
112 ZFP64  
Reconstituted Complex Homo sapiens
113 SOX4  
Reconstituted Complex Homo sapiens
114 IL17F  
Reconstituted Complex Homo sapiens
115 BDNF 627
Reconstituted Complex Homo sapiens
116 INSM2 84684
Reconstituted Complex Homo sapiens
117 DMRT2  
Reconstituted Complex Homo sapiens
118 PLOD3 8985
Reconstituted Complex Homo sapiens
119 EIF4B 1975
Reconstituted Complex Homo sapiens
120 PXN 5829
Reconstituted Complex Homo sapiens
121 PGK1 5230
Reconstituted Complex Homo sapiens
122 SEMA3G 56920
Reconstituted Complex Homo sapiens
123 ZNF625  
Reconstituted Complex Homo sapiens
124 ELP5 23587
Reconstituted Complex Homo sapiens
125 OXCT2  
Reconstituted Complex Homo sapiens
126 DSCR8  
Reconstituted Complex Homo sapiens
127 SEPT6 23157
Reconstituted Complex Homo sapiens
128 ESYT1 23344
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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