Gene description for ME3
Gene name malic enzyme 3, NADP(+)-dependent, mitochondrial
Gene symbol ME3
Other names/aliases NADP-ME
Species Homo sapiens
 Database cross references - ME3
ExoCarta ExoCarta_10873
Vesiclepedia VP_10873
Entrez Gene 10873
HGNC 6985
MIM 604626
UniProt Q16798  
 ME3 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for ME3
Molecular Function
    malic enzyme activity GO:0004470 IDA
    malate dehydrogenase (decarboxylating) (NADP+) activity GO:0004473 IBA
    malate dehydrogenase (decarboxylating) (NADP+) activity GO:0004473 IDA
    oxaloacetate decarboxylase activity GO:0008948 IEA
    metal ion binding GO:0046872 IEA
    NAD binding GO:0051287 IEA
    NADP+ binding GO:0070401 IDA
Biological Process
    pyruvate metabolic process GO:0006090 IBA
    pyruvate metabolic process GO:0006090 IDA
    malate metabolic process GO:0006108 IBA
    malate metabolic process GO:0006108 IDA
    aerobic respiration GO:0009060 TAS
    oxygen metabolic process GO:0072592 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 TAS
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ME3 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ME3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOU 58480
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which ME3 is involved
PathwayEvidenceSource
Aerobic respiration and respiratory electron transport TAS Reactome
Metabolism TAS Reactome
Pyruvate metabolism TAS Reactome





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