Gene description for ME3
Gene name malic enzyme 3, NADP(+)-dependent, mitochondrial
Gene symbol ME3
Other names/aliases NADP-ME
Species Homo sapiens
 Database cross references - ME3
ExoCarta ExoCarta_10873
Entrez Gene 10873
HGNC 6985
MIM 604626
UniProt Q16798  
 ME3 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for ME3
Molecular Function
    oxaloacetate decarboxylase activity GO:0008948 IEA
    cofactor binding GO:0048037 TAS
    malate dehydrogenase (decarboxylating) (NADP+) activity GO:0004473 IDA
    malic enzyme activity GO:0004470 IDA
    malate dehydrogenase (decarboxylating) (NAD+) activity GO:0004471 IEA
    metal ion binding GO:0046872 IEA
    NAD binding GO:0051287 IEA
Biological Process
    oxidation-reduction process GO:0055114 IDA
    pyruvate metabolic process GO:0006090 IDA
    oxygen metabolic process GO:0072592 TAS
    malate metabolic process GO:0006108 IDA
    aerobic respiration GO:0009060 TAS
Subcellular Localization
    mitochondrial matrix GO:0005759 IEA
    mitochondrion GO:0005739 TAS
 Experiment description of studies that identified ME3 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ME3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ME3 is involved
No pathways found





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