Experiment description of studies that identified SEPT6 in exosomes

1 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

6 Experiment ID 191 MISEV standards ✘ Biophysical techniques ✔ Alix|CD81|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for SEPT6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HK2 3099 Co-fractionation Homo sapiens 2 ANLN 54443 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 HSPA5 3309 Affinity Capture-MS Homo sapiens 4 SEPT1 1731 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 5 RHOU 58480 Reconstituted Complex Homo sapiens 6 SEPT14   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 TK1 7083 Two-hybrid Homo sapiens 8 PTMA 5757 Cross-Linking-MS (XL-MS) Homo sapiens 9 XPO1 7514 Affinity Capture-MS Homo sapiens 10 CDC42EP2   Reconstituted Complex Homo sapiens 11 RAP1GDS1 5910 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 SEPT4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 SEPT3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 14 CEP72   Affinity Capture-MS Homo sapiens 15 UBA1 7317 Co-fractionation Homo sapiens 16 KIAA1429 25962 Affinity Capture-MS Homo sapiens 17 SEPT5 5413 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 PLEKHF2 79666 Two-hybrid Homo sapiens 19 SEPT12   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 SEPT10 151011 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 SEPT11 55752 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 Sept7   Affinity Capture-MS Mus musculus 23 SOCS7   Affinity Capture-Western Homo sapiens 24 SEPT2 4735 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 ACAP3 116983 Affinity Capture-MS Homo sapiens 26 SEPT9 10801 Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 Sept11   Affinity Capture-MS Mus musculus 28 Sept2   Affinity Capture-MS Mus musculus 29 KDM1A 23028 Two-hybrid Homo sapiens 30 MORN4   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 31 STAT1 6772 Proximity Label-MS Homo sapiens 32 RPA3 6119 Proximity Label-MS Homo sapiens 33 DYNC1LI2 1783 Proximity Label-MS Homo sapiens 34 TRIM21 6737 Affinity Capture-MS Homo sapiens 35 SEPT8 23176 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 COPB2 9276 Affinity Capture-MS Homo sapiens 37 LAMP1 3916 Proximity Label-MS Homo sapiens 38 FLNB 2317 Co-fractionation Homo sapiens 39 TRIM32 22954 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 BICD2 23299 Proximity Label-MS Homo sapiens 41 CUL7 9820 Affinity Capture-MS Homo sapiens 42 MRPL12 6182 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 SH3KBP1 30011 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 44 WHSC1L1   Two-hybrid Homo sapiens 45 SEPT7 989 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 46 CEBPA   Protein-peptide Homo sapiens 47 CDC42EP3 10602 Reconstituted Complex Homo sapiens 48 Bub1   Affinity Capture-MS Mus musculus 49 Sept9   Affinity Capture-MS Mus musculus 50 KRAS 3845 Proximity Label-MS Homo sapiens 51 CDC42EP4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which SEPT6 is involved

No pathways found