Gene description for TRIM21
Gene name tripartite motif containing 21
Gene symbol TRIM21
Other names/aliases RNF81
RO52
Ro/SSA
SSA
SSA1
Species Homo sapiens
 Database cross references - TRIM21
ExoCarta ExoCarta_6737
Vesiclepedia VP_6737
Entrez Gene 6737
HGNC 11312
MIM 109092
UniProt P19474  
 TRIM21 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TRIM21
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
    DNA binding GO:0003677 IEA
    zinc ion binding GO:0008270 IEA
    ligase activity GO:0016874 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    regulation of type I interferon production GO:0032479 TAS
    positive regulation of viral entry into host cell GO:0046598 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    innate immune response GO:0045087 TAS
    positive regulation of cell cycle GO:0045787 IMP
    protein trimerization GO:0070206 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    negative regulation of protein deubiquitination GO:0090086 IMP
    protein ubiquitination GO:0016567 IDA
    protein monoubiquitination GO:0006513 IDA
    negative regulation of viral transcription GO:0032897 IDA
    protein destabilization GO:0031648 IMP
    cell cycle GO:0007049 IEA
    cytokine-mediated signaling pathway GO:0019221 TAS
    protein polyubiquitination GO:0000209 IDA
    negative regulation of viral release from host cell GO:1902187 IDA
    protein autoubiquitination GO:0051865 IDA
    positive regulation of type I interferon production GO:0032481 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IDA
    cytoplasmic mRNA processing body GO:0000932 IEA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    ribonucleoprotein complex GO:0030529 TAS
 Experiment description of studies that identified TRIM21 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRIM21
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USP4 7375
Two-hybrid Homo sapiens
2 NIF3L1 60491
Two-hybrid Homo sapiens
3 TRIM21 6737
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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