Gene description for CD14
Gene name CD14 molecule
Gene symbol CD14
Other names/aliases -
Species Homo sapiens
 Database cross references - CD14
ExoCarta ExoCarta_929
Vesiclepedia VP_929
Entrez Gene 929
HGNC 1628
MIM 158120
UniProt P08571  
 CD14 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for CD14
Molecular Function
    lipopolysaccharide binding GO:0001530 IBA
    lipopolysaccharide binding GO:0001530 IDA
    opsonin receptor activity GO:0001847 TAS
    lipopolysaccharide immune receptor activity GO:0001875 IDA
    protein binding GO:0005515 IPI
    peptidoglycan immune receptor activity GO:0016019 TAS
    lipoteichoic acid binding GO:0070891 IDA
Biological Process
    positive regulation of cytokine production GO:0001819 IBA
    cell surface pattern recognition receptor signaling pathway GO:0002752 IDA
    receptor-mediated endocytosis GO:0006898 IEA
    phagocytosis GO:0006909 TAS
    apoptotic process GO:0006915 TAS
    inflammatory response GO:0006954 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IEA
    response to magnesium ion GO:0032026 IEA
    positive regulation of type I interferon production GO:0032481 IEA
    positive regulation of type II interferon production GO:0032729 ISS
    positive regulation of interleukin-8 production GO:0032757 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    toll-like receptor 4 signaling pathway GO:0034142 IBA
    toll-like receptor 4 signaling pathway GO:0034142 IDA
    positive regulation of toll-like receptor 4 signaling pathway GO:0034145 ISS
    response to tumor necrosis factor GO:0034612 IEA
    innate immune response GO:0045087 IEA
    response to ethanol GO:0045471 IEA
    positive regulation of endocytosis GO:0045807 IEA
    response to electrical stimulus GO:0051602 IEA
    cellular response to molecule of bacterial origin GO:0071219 IDA
    cellular response to lipopolysaccharide GO:0071222 IBA
    cellular response to lipopolysaccharide GO:0071222 IDA
    cellular response to lipopolysaccharide GO:0071222 IMP
    cellular response to lipoteichoic acid GO:0071223 IDA
    cellular response to diacyl bacterial lipopeptide GO:0071726 IDA
    cellular response to triacyl bacterial lipopeptide GO:0071727 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    endosome membrane GO:0010008 TAS
    secretory granule membrane GO:0030667 TAS
    membrane raft GO:0045121 IBA
    membrane raft GO:0045121 IDA
    lipopolysaccharide receptor complex GO:0046696 IBA
    lipopolysaccharide receptor complex GO:0046696 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CD14 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
9
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CD14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP3S1 1176
Affinity Capture-MS Homo sapiens
2 PHB 5245
Affinity Capture-Western Homo sapiens
3 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
4 TRIM21 6737
Affinity Capture-Western Homo sapiens
5 LAMB1 3912
Affinity Capture-MS Homo sapiens
6 GJB2  
Two-hybrid Homo sapiens
7 OASL 8638
Affinity Capture-MS Homo sapiens
8 GALNS 2588
Affinity Capture-MS Homo sapiens
9 TNFRSF1B  
Two-hybrid Homo sapiens
10 BCHE 590
Affinity Capture-MS Homo sapiens
11 ARID2  
Affinity Capture-MS Homo sapiens
12 MAN2B1 4125
Affinity Capture-MS Homo sapiens
13 SVIL 6840
Affinity Capture-MS Homo sapiens
14 POLR2J  
Two-hybrid Homo sapiens
15 EXO1  
Affinity Capture-MS Homo sapiens
16 ANXA7 310
Affinity Capture-MS Homo sapiens
17 PHF20L1  
Affinity Capture-MS Homo sapiens
18 ARFGAP3 26286
Two-hybrid Homo sapiens
19 MTG2  
Affinity Capture-MS Homo sapiens
20 HPS5 11234
Affinity Capture-MS Homo sapiens
21 SKP2  
Affinity Capture-Western Homo sapiens
22 BUB1B  
Affinity Capture-MS Homo sapiens
23 PPP3R2  
Two-hybrid Homo sapiens
24 SERPINE1 5054
Affinity Capture-MS Homo sapiens
25 TAF1  
Affinity Capture-MS Homo sapiens
26 DDX58 23586
Affinity Capture-RNA Homo sapiens
27 FNDC5  
Affinity Capture-MS Homo sapiens
28 BCAS3 54828
Affinity Capture-MS Homo sapiens
29 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
30 TAF9 6880
Affinity Capture-MS Homo sapiens
31 C2orf44  
Affinity Capture-MS Homo sapiens
32 FBXO2 26232
Affinity Capture-MS Homo sapiens
33 DIS3 22894
Affinity Capture-MS Homo sapiens
34 INTS7  
Affinity Capture-MS Homo sapiens
35 PHF21A  
Affinity Capture-MS Homo sapiens
36 TLR2 7097
Affinity Capture-Western Homo sapiens
37 ERAP1 51752
Affinity Capture-MS Homo sapiens
38 RNF133  
Affinity Capture-MS Homo sapiens
39 LY6E  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 LTF 4057
Reconstituted Complex Homo sapiens
41 ICAM5 7087
Affinity Capture-MS Homo sapiens
42 IRAK3 11213
Two-hybrid Homo sapiens
43 LBP 3929
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
44 CERCAM 51148
Affinity Capture-MS Homo sapiens
45 TXNDC11 51061
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CD14 is involved
PathwayEvidenceSource
Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) TAS Reactome
Adaptive Immune System TAS Reactome
Antigen processing-Cross presentation TAS Reactome
Apoptosis TAS Reactome
Caspase activation via Death Receptors in the presence of ligand TAS Reactome
Caspase activation via extrinsic apoptotic signalling pathway TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Disease TAS Reactome
Diseases associated with the TLR signaling cascade TAS Reactome
Diseases of Immune System TAS Reactome
ER-Phagosome pathway TAS Reactome
IKK complex recruitment mediated by RIP1 TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation TAS Reactome
IRAK4 deficiency (TLR2/4) TAS Reactome
MyD88 deficiency (TLR2/4) TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Neutrophil degranulation TAS Reactome
Programmed Cell Death TAS Reactome
Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 TAS Reactome
Regulation of TLR by endogenous ligand TAS Reactome
Respiratory syncytial virus (RSV) attachment and entry TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
RSV-host interactions TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRAF6-mediated induction of TAK1 complex within TLR4 complex TAS Reactome
Transfer of LPS from LBP carrier to CD14 TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
TRIF-mediated programmed cell death TAS Reactome
Viral Infection Pathways TAS Reactome





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