Gene description for TLR2
Gene name toll-like receptor 2
Gene symbol TLR2
Other names/aliases CD282
TIL4
Species Homo sapiens
 Database cross references - TLR2
ExoCarta ExoCarta_7097
Vesiclepedia VP_7097
Entrez Gene 7097
HGNC 11848
MIM 603028
UniProt O60603  
 TLR2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast milk 17641064    
 Gene ontology annotations for TLR2
Molecular Function
    triacyl lipopeptide binding GO:0042497 IDA
    lipopolysaccharide receptor activity GO:0001875 TAS
    signaling pattern recognition receptor activity GO:0008329 IDA
    diacyl lipopeptide binding GO:0042498 IEA
    transmembrane signaling receptor activity GO:0004888 IEA
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IDA
    lipoteichoic acid binding GO:0070891 IEA
    peptidoglycan binding GO:0042834 IDA
    receptor activity GO:0004872 IDA
Biological Process
    negative regulation of cell proliferation GO:0008285 IEA
    negative regulation of interleukin-12 production GO:0032695 IEA
    nitric oxide metabolic process GO:0046209 IEA
    response to insulin GO:0032868 IEA
    positive regulation of inflammatory response GO:0050729 IC
    I-kappaB phosphorylation GO:0007252 IDA
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    inflammatory response GO:0006954 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    positive regulation of chemokine production GO:0032722 IDA
    positive regulation of interleukin-10 production GO:0032733 IEA
    cellular response to bacterial lipopeptide GO:0071221 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    interleukin-10 production GO:0032613 IDA
    toll-like receptor signaling pathway GO:0002224 TAS
    signal transduction GO:0007165 TAS
    negative regulation of interleukin-17 production GO:0032700 IEA
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of leukocyte migration GO:0002687 IEA
    positive regulation of macrophage cytokine production GO:0060907 IEA
    cytokine secretion involved in immune response GO:0002374 IMP
    positive regulation of interferon-beta production GO:0032728 ISS
    defense response to Gram-positive bacterium GO:0050830 IDA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    positive regulation of toll-like receptor signaling pathway GO:0034123 IDA
    lipopolysaccharide-mediated signaling pathway GO:0031663 TAS
    chloramphenicol transport GO:0042892 IEA
    positive regulation of NF-kappaB import into nucleus GO:0042346 IDA
    response to molecule of fungal origin GO:0002238 IEA
    immune response GO:0006955 TAS
    positive regulation of cytokine secretion GO:0050715 IEA
    positive regulation of interleukin-12 production GO:0032735 ISS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    negative regulation of growth of symbiont in host GO:0044130 IEA
    detection of diacyl bacterial lipopeptide GO:0042496 IDA
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    cellular response to diacyl bacterial lipopeptide GO:0071726 IDA
    apoptotic process GO:0006915 TAS
    detection of triacyl bacterial lipopeptide GO:0042495 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    cellular response to lipoteichoic acid GO:0071223 IDA
    cellular response to peptidoglycan GO:0071224 IEA
    positive regulation of interleukin-6 production GO:0032755 IDA
    central nervous system myelin formation GO:0032289 IEA
    positive regulation of interleukin-18 production GO:0032741 ISS
    induction by symbiont of defense-related host nitric oxide production GO:0052063 IEA
    innate immune response GO:0045087 TAS
    positive regulation of tumor necrosis factor biosynthetic process GO:0042535 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    response to toxic substance GO:0009636 IEA
    response to fatty acid GO:0070542 IEA
    positive regulation of oligodendrocyte differentiation GO:0048714 IEA
    cell surface pattern recognition receptor signaling pathway GO:0002752 IEA
    cellular response to triacyl bacterial lipopeptide GO:0071727 IDA
    response to progesterone GO:0032570 IEA
    response to hypoxia GO:0001666 IEA
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 ISS
    microglial cell activation GO:0001774 IEA
    leukotriene metabolic process GO:0006691 IEA
Subcellular Localization
    cell projection GO:0042995 IEA
    plasma membrane GO:0005886 TAS
    Toll-like receptor 1-Toll-like receptor 2 protein complex GO:0035354 IDA
    Toll-like receptor 2-Toll-like receptor 6 protein complex GO:0035355 IEA
    integral component of plasma membrane GO:0005887 TAS
    cytoplasm GO:0005737 IDA
    cell body GO:0044297 IEA
    cell surface GO:0009986 IDA
    external side of plasma membrane GO:0009897 IEA
 Experiment description of studies that identified TLR2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 48
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for TLR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD14 929
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
2 TOLLIP 54472
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 IRAK1  
Invivo Homo sapiens
4 LY96  
Affinity Capture-Western Homo sapiens
5 MYD88 4615
Two-hybrid Homo sapiens
6 HSP90B1 7184
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 TLR1 7096
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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