Gene description for MYD88
Gene name myeloid differentiation primary response 88
Gene symbol MYD88
Other names/aliases MYD88D
Species Homo sapiens
 Database cross references - MYD88
ExoCarta ExoCarta_4615
Vesiclepedia VP_4615
Entrez Gene 4615
HGNC 7562
MIM 602170
UniProt Q99836  
 MYD88 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MYD88
Molecular Function
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    death receptor binding GO:0005123 TAS
    TIR domain binding GO:0070976 IPI
Biological Process
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    response to interleukin-1 GO:0070555 IMP
    signal transduction GO:0007165 NAS
    negative regulation of apoptotic process GO:0043066 TAS
    positive regulation of interleukin-17 production GO:0032740 ISS
    cell surface receptor signaling pathway GO:0007166 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    defense response to Gram-positive bacterium GO:0050830 ISS
    positive regulation of interleukin-6 production GO:0032755 ISS
    positive regulation of type I interferon production GO:0032481 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    regulation of inflammatory response GO:0050727 ISS
    3'-UTR-mediated mRNA stabilization GO:0070935 IDA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    innate immune response GO:0045087 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    positive regulation of interleukin-23 production GO:0032747 ISS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    inflammatory response GO:0006954 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    endosome membrane GO:0010008 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified MYD88 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYD88
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TLR2 7097
Two-hybrid Homo sapiens
2 FADD 8772
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 TLR9  
Two-hybrid Homo sapiens
4 IRAK4 51135
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 IRAK2  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
6 RAC1 5879
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 IRAK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 SPOP  
Two-hybrid Homo sapiens
9 TLR5  
Two-hybrid Homo sapiens
10 TLR4  
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
11 IRAK3 11213
Affinity Capture-Western Homo sapiens
12 IL1RAP 3556
Invivo Homo sapiens
13 TLR3  
Reconstituted Complex Homo sapiens
14 TIRAP  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
15 IL1R1 3554
Affinity Capture-Western Homo sapiens
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