Gene description for TRAF3
Gene name TNF receptor-associated factor 3
Gene symbol TRAF3
Other names/aliases CAP-1
CAP1
CD40bp
CRAF1
IIAE5
LAP1
Species Homo sapiens
 Database cross references - TRAF3
ExoCarta ExoCarta_7187
Vesiclepedia VP_7187
Entrez Gene 7187
HGNC 12033
MIM 601896
UniProt Q13114  
 TRAF3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TRAF3
Molecular Function
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 TAS
    ubiquitin protein ligase binding GO:0031625 IPI
    zinc ion binding GO:0008270 IEA
    protein kinase binding GO:0019901 IEA
    thioesterase binding GO:0031996 IPI
    ubiquitin-protein transferase activity GO:0004842 IEA
    tumor necrosis factor receptor binding GO:0005164 IPI
Biological Process
    regulation of apoptotic process GO:0042981 IEA
    regulation of proteolysis GO:0030162 IMP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    protein ubiquitination GO:0016567 IEA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    regulation of cytokine production GO:0001817 ISS
    innate immune response GO:0045087 TAS
    Toll signaling pathway GO:0008063 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    regulation of defense response to virus GO:0050688 ISS
    apoptotic process GO:0006915 IEA
    negative regulation of type I interferon production GO:0032480 TAS
    regulation of interferon-beta production GO:0032648 ISS
    tumor necrosis factor-mediated signaling pathway GO:0033209 IMP
    signal transduction GO:0007165 TAS
Subcellular Localization
    endosome GO:0005768 IEA
    mitochondrion GO:0005739 IEA
    cytoplasmic side of plasma membrane GO:0009898 ISS
    cytosol GO:0005829 TAS
    CD40 receptor complex GO:0035631 ISS
 Experiment description of studies that identified TRAF3 in exosomes
1
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for TRAF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD40 958
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
2 MAP3K14  
Invitro Homo sapiens
3 TANK 10010
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
4 TRAF3IP2  
Invivo Homo sapiens
5 USP7 7874
Invitro Homo sapiens
6 CFLAR  
Affinity Capture-Western Homo sapiens
7 TNFRSF4  
Invivo Homo sapiens
Two-hybrid Homo sapiens
8 TRADD 8717
Invivo Homo sapiens
Two-hybrid Homo sapiens
9 LTBR 4055
Affinity Capture-Western Homo sapiens
10 TRAF3IP1 26146
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
11 NUP62 23636
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 CAP1 10487
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 TNFSF14  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 TNFRSF7  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
15 TNFRSF8 943
Invivo Homo sapiens
16 TNFRSF18  
Affinity Capture-Western Homo sapiens
17 RIPK1 8737
Invivo Homo sapiens
Two-hybrid Homo sapiens
18 TNFRSF17  
Invivo Homo sapiens
19 MAP3K5 4217
Affinity Capture-Western Homo sapiens
20 TNFRSF11A  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
21 TRIM37  
Invitro Homo sapiens
22 EDA2R  
Invitro Homo sapiens
23 NGFR 4804
Affinity Capture-Western Homo sapiens
24 CASP3 836
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
25 TNFRSF9  
Reconstituted Complex Homo sapiens
26 SRC 6714
Affinity Capture-Western Homo sapiens
27 TDP2 51567
Affinity Capture-Western Homo sapiens
28 TNFRSF19  
Invivo Homo sapiens
29 TNFRSF14  
Invivo Homo sapiens
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