Gene description for PPP3R1
Gene name protein phosphatase 3, regulatory subunit B, alpha
Gene symbol PPP3R1
Other names/aliases CALNB1
CNB
CNB1
Species Homo sapiens
 Database cross references - PPP3R1
ExoCarta ExoCarta_5534
Vesiclepedia VP_5534
Entrez Gene 5534
HGNC 9317
MIM 601302
UniProt P63098  
 PPP3R1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PPP3R1
Molecular Function
    calcium-dependent protein serine/threonine phosphatase activity GO:0004723 NAS
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 NAS
    calcium-dependent protein serine/threonine phosphatase regulator activity GO:0008597 IBA
    phosphatase binding GO:0019902 IBA
    phosphatase binding GO:0019902 IPI
    protein domain specific binding GO:0019904 IPI
Biological Process
    branching involved in blood vessel morphogenesis GO:0001569 IEA
    epithelial to mesenchymal transition GO:0001837 IEA
    protein import into nucleus GO:0006606 IEA
    heart development GO:0007507 IEA
    myelination in peripheral nervous system GO:0022011 IEA
    calcineurin-NFAT signaling cascade GO:0033173 IBA
    calcineurin-NFAT signaling cascade GO:0033173 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    lung epithelial cell differentiation GO:0060487 IEA
    positive regulation of calcineurin-NFAT signaling cascade GO:0070886 NAS
    regulation of synaptic vesicle cycle GO:0098693 IEA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IEA
    positive regulation of calcium ion import across plasma membrane GO:1905665 NAS
    negative regulation of calcium ion import across plasma membrane GO:1905949 NAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    calcineurin complex GO:0005955 IBA
    calcineurin complex GO:0005955 IDA
    calcineurin complex GO:0005955 NAS
    protein serine/threonine phosphatase complex GO:0008287 NAS
    sarcolemma GO:0042383 IEA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    parallel fiber to Purkinje cell synapse GO:0098688 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified PPP3R1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PPP3R1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPM6A 2823
Affinity Capture-MS Homo sapiens
2 HAX1  
Affinity Capture-MS Homo sapiens
3 PPP3CA 5530
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
4 CABP2  
Reconstituted Complex Homo sapiens
5 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DGCR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DNAJC8 22826
Affinity Capture-MS Homo sapiens
8 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TP53 7157
Affinity Capture-MS Homo sapiens
10 RASSF2 9770
Affinity Capture-MS Homo sapiens
11 NAP1L2  
Two-hybrid Homo sapiens
12 TNS4 84951
Affinity Capture-MS Homo sapiens
13 RCAN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CALM2 805
Affinity Capture-MS Homo sapiens
15 PMEPA1 56937
Affinity Capture-MS Homo sapiens
16 TRIM41 90933
Affinity Capture-MS Homo sapiens
17 IQCB1  
Affinity Capture-MS Homo sapiens
18 TMCC3  
Affinity Capture-MS Homo sapiens
19 FAM163B  
Affinity Capture-MS Homo sapiens
20 PDE4D  
Affinity Capture-Western Homo sapiens
21 Cul3  
Affinity Capture-MS Mus musculus
22 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
23 CSN2 1447
Affinity Capture-MS Homo sapiens
24 FCGR2A 2212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PPIA 5478
Co-crystal Structure Homo sapiens
26 FAM122A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CRTC2 200186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TRAF3 7187
Affinity Capture-Western Homo sapiens
30 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TMEM200A 114801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GSK3A 2931
Affinity Capture-MS Homo sapiens
33 SOX15  
Affinity Capture-MS Homo sapiens
34 KCNK18  
Affinity Capture-MS Homo sapiens
35 HOXC5  
Affinity Capture-MS Homo sapiens
36 PRR3  
Affinity Capture-MS Homo sapiens
37 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
38 SRBD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SPATA33  
Affinity Capture-MS Homo sapiens
40 PSMA7 5688
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
41 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CABP1 9478
Reconstituted Complex Homo sapiens
44 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 NFATC1 4772
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PPP3CB  
Affinity Capture-MS Homo sapiens
47 CDR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SMPD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 LIME1 54923
Affinity Capture-MS Homo sapiens
50 RCAN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MYH7 4625
Affinity Capture-MS Homo sapiens
52 IGFBP6 3489
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TINF2  
Affinity Capture-MS Homo sapiens
54 CALM3 808
Affinity Capture-MS Homo sapiens
55 RCAN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PPP3CC 5533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
57 CABP5  
Reconstituted Complex Homo sapiens
58 HYPM  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here