Gene description for PMEPA1
Gene name prostate transmembrane protein, androgen induced 1
Gene symbol PMEPA1
Other names/aliases STAG1
TMEPAI
Species Homo sapiens
 Database cross references - PMEPA1
ExoCarta ExoCarta_56937
Vesiclepedia VP_56937
Entrez Gene 56937
HGNC 14107
MIM 606564
UniProt Q969W9  
 PMEPA1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PMEPA1
Molecular Function
    protein binding GO:0005515 IPI
    WW domain binding GO:0050699 IPI
    R-SMAD binding GO:0070412 IBA
    R-SMAD binding GO:0070412 IPI
    protein sequestering activity GO:0140311 IDA
Biological Process
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IBA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IMP
    androgen receptor signaling pathway GO:0030521 NAS
    negative regulation of SMAD protein signal transduction GO:0060392 IDA
    negative regulation of SMAD protein signal transduction GO:0060392 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 IDA
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 IDA
    membrane GO:0016020 NAS
    early endosome membrane GO:0031901 IBA
    early endosome membrane GO:0031901 IDA
    early endosome membrane GO:0031901 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified PMEPA1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PMEPA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBR2 23304
Affinity Capture-MS Homo sapiens
2 JCHAIN 3512
Affinity Capture-MS Homo sapiens
3 PPP3CA 5530
Affinity Capture-MS Homo sapiens
4 PPP3R1 5534
Affinity Capture-MS Homo sapiens
5 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
6 NEDD4 4734
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 DCTN6 10671
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
8 IGHA1 3493
Affinity Capture-MS Homo sapiens
9 UBQLN2 29978
Two-hybrid Homo sapiens
10 PHLPP1  
Affinity Capture-Western Homo sapiens
11 NFKBIA  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 IGKC 3514
Affinity Capture-MS Homo sapiens
13 IGHA2 3494
Affinity Capture-MS Homo sapiens
14 CAND1 55832
Affinity Capture-MS Homo sapiens
15 DCTN5 84516
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
16 BPIFA1 51297
Affinity Capture-MS Homo sapiens
17 ACP2 53
Affinity Capture-MS Homo sapiens
18 HP 3240
Affinity Capture-MS Homo sapiens
19 CST4 1472
Affinity Capture-MS Homo sapiens
20 STXBP4 252983
Affinity Capture-MS Homo sapiens
21 UBASH3A 53347
Two-hybrid Homo sapiens
22 ZG16B 124220
Affinity Capture-MS Homo sapiens
23 BPIFB1 92747
Affinity Capture-MS Homo sapiens
24 PPP3CB  
Affinity Capture-MS Homo sapiens
25 UBQLN4 56893
Two-hybrid Homo sapiens
26 PRR4 11272
Affinity Capture-MS Homo sapiens
27 PPP3CC 5533
Affinity Capture-MS Homo sapiens
28 HGS 9146
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 LCN1 3933
Affinity Capture-MS Homo sapiens
30 SGTA 6449
Two-hybrid Homo sapiens
31 AR 367
Affinity Capture-Western Homo sapiens
32 LTF 4057
Affinity Capture-MS Homo sapiens
33 LACRT 90070
Affinity Capture-MS Homo sapiens
34 NEDD4L 23327
Affinity Capture-MS Homo sapiens
35 PIGR 5284
Affinity Capture-MS Homo sapiens
36 STAM 8027
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 DMBT1 1755
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here