Gene description for DCTN5
Gene name dynactin 5 (p25)
Gene symbol DCTN5
Other names/aliases -
Species Homo sapiens
 Database cross references - DCTN5
ExoCarta ExoCarta_84516
Vesiclepedia VP_84516
Entrez Gene 84516
HGNC 24594
MIM 612962
UniProt Q9BTE1  
 DCTN5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for DCTN5
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    ventricular septum development GO:0003281 IEA
    aorta development GO:0035904 IEA
    coronary vasculature development GO:0060976 IEA
Subcellular Localization
    kinetochore GO:0000776 IEA
    nucleoplasm GO:0005654 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    dynactin complex GO:0005869 IBA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified DCTN5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DCTN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAPZA1 829
Affinity Capture-MS Homo sapiens
2 ZRANB1 54764
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 IKBKAP 8518
Co-fractionation Homo sapiens
5 GSN 2934
Affinity Capture-MS Homo sapiens
6 RPS6KA2  
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PRKCSH 5589
Co-fractionation Homo sapiens
9 CLIP1 6249
Affinity Capture-MS Homo sapiens
10 PDGFD 80310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PREP 5550
Co-fractionation Homo sapiens
12 NASP 4678
Co-fractionation Homo sapiens
13 Dctn1 13191
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
14 DCTN6 10671
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CXCL14  
Affinity Capture-MS Homo sapiens
16 NINL  
Two-hybrid Homo sapiens
17 CCT2 10576
Affinity Capture-MS Homo sapiens
18 C1orf106  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 FATE1  
Two-hybrid Homo sapiens
20 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PMEPA1 56937
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
22 USP5 8078
Co-fractionation Homo sapiens
23 LAMP1 3916
Affinity Capture-MS Homo sapiens
24 MIS18A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 Myo1c 17913
Affinity Capture-MS Mus musculus
26 DBN1 1627
Affinity Capture-MS Homo sapiens
27 MCM7 4176
Co-fractionation Homo sapiens
28 SEPT14  
Affinity Capture-MS Homo sapiens
29 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ACTR1B 10120
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
31 DCTN2 10540
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TCP1 6950
Affinity Capture-MS Homo sapiens
33 RIC8A 60626
Affinity Capture-MS Homo sapiens
34 CSPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PRMT5 10419
Co-fractionation Homo sapiens
36 COPE 11316
Affinity Capture-MS Homo sapiens
37 CPVL 54504
Affinity Capture-MS Homo sapiens
38 CCT6B 10693
Affinity Capture-MS Homo sapiens
39 MAPRE1 22919
Affinity Capture-MS Homo sapiens
40 DCTN3 11258
Affinity Capture-MS Homo sapiens
41 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
42 N4BP2L1  
Affinity Capture-MS Homo sapiens
43 Dctn2 69654
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
44 TUBB8 347688
Affinity Capture-MS Homo sapiens
45 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TXN 7295
Co-fractionation Homo sapiens
47 WDHD1  
Co-fractionation Homo sapiens
48 Actr1b 226977
Affinity Capture-MS Mus musculus
49 IL17B 27190
Affinity Capture-MS Homo sapiens
50 Dctn3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
51 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
52 HARS 3035
Co-fractionation Homo sapiens
53 DCTN4 51164
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
54 COPB2 9276
Co-fractionation Homo sapiens
55 DEFB127  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DCTN1 1639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ACTB 60
Affinity Capture-MS Homo sapiens
58 Actr1a 54130
Affinity Capture-MS Mus musculus
59 KRAS 3845
Negative Genetic Homo sapiens
60 ACTR1A 10121
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
61 UQCRH 7388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ACTR10 55860
Affinity Capture-MS Homo sapiens
63 RBPMS 11030
Two-hybrid Homo sapiens
64 ACTG1 71
Affinity Capture-MS Homo sapiens
65 MED22  
Affinity Capture-MS Homo sapiens
66 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 Dctn4  
Affinity Capture-MS Mus musculus
68 TPM3 7170
Co-fractionation Homo sapiens
69 CRNN 49860
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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