Gene description for Dctn2
Gene name dynactin 2
Gene symbol Dctn2
Other names/aliases 2310042E05Rik
C130077D06Rik
C87049
DCTN-50
GMP23-48K
RBP50
p50
Species Mus musculus
 Database cross references - Dctn2
ExoCarta ExoCarta_69654
Vesiclepedia VP_69654
Entrez Gene 69654
UniProt Q99KJ8  
 Dctn2 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
 Gene ontology annotations for Dctn2
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    spectrin binding GO:0030507 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    mitotic spindle organization GO:0007052 IBA
    mitotic spindle organization GO:0007052 IEA
    mitotic spindle organization GO:0007052 ISO
    mitotic metaphase chromosome alignment GO:0007080 IEA
    mitotic metaphase chromosome alignment GO:0007080 ISO
    melanosome transport GO:0032402 IMP
    protein localization to centrosome GO:0071539 IEA
    protein localization to centrosome GO:0071539 ISO
Subcellular Localization
    kinetochore GO:0000776 IEA
    kinetochore GO:0000776 ISO
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    dynactin complex GO:0005869 IBA
    dynactin complex GO:0005869 IEA
    dynactin complex GO:0005869 ISO
    microtubule GO:0005874 IEA
    membrane GO:0016020 IEA
    dynein complex GO:0030286 IEA
    growth cone GO:0030426 IDA
    vesicle GO:0031982 ISO
    vesicle GO:0031982 ISS
 Experiment description of studies that identified Dctn2 in exosomes
1
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Dctn2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphs2  
Affinity Capture-MS Mus musculus
2 SNCA 6622
Affinity Capture-MS Homo sapiens
3 DCTN3 11258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 Dynlt3 67117
Co-fractionation Mus musculus
5 Bicd2  
Co-fractionation Mus musculus
6 DCTN6 10671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 EEA1 8411
Affinity Capture-MS Homo sapiens
8 Atxn1  
Affinity Capture-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 CAPZA1 829
Affinity Capture-MS Homo sapiens
11 CAPZA2 830
Affinity Capture-MS Homo sapiens
12 ACTR1B 10120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 DCTN2 10540
Affinity Capture-MS Homo sapiens
14 DCTN5 84516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 Mettl21e  
Affinity Capture-MS Mus musculus
16 Inpp5d 16331
Affinity Capture-MS Mus musculus
17 Tmem173  
Proximity Label-MS Mus musculus
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 Uso1 56041
Co-fractionation Mus musculus
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 DCTN4 51164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DCTN1 1639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TMSB4X 7114
Affinity Capture-MS Homo sapiens
24 ACTR1A 10121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GOLM1 51280
Affinity Capture-MS Homo sapiens
26 SHCBP1 79801
Affinity Capture-MS Homo sapiens
27 Mapt  
Affinity Capture-MS Mus musculus
28 ACTR10 55860
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KLHL2 11275
Affinity Capture-MS Homo sapiens
30 TRIM47 91107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Dctn2 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
G2/M Transition IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Prometaphase IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here