Gene description for KLHL2
Gene name kelch-like family member 2
Gene symbol KLHL2
Other names/aliases ABP-KELCH
MAV
MAYVEN
Species Homo sapiens
 Database cross references - KLHL2
ExoCarta ExoCarta_11275
Vesiclepedia VP_11275
Entrez Gene 11275
HGNC 6353
MIM 605774
UniProt O95198  
 KLHL2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for KLHL2
Molecular Function
    actin binding GO:0003779 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IBA
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    ruffle GO:0001726 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    actin cytoskeleton GO:0015629 IDA
    lamellipodium GO:0030027 IEA
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 ISS
 Experiment description of studies that identified KLHL2 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for KLHL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WNK4  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
2 DCTN6 10671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CWC22 57703
Two-hybrid Homo sapiens
4 IMPDH1 3614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PADI6  
Two-hybrid Homo sapiens
6 BCAM 4059
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 DCTN3 11258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SDCBP 6386
Two-hybrid Homo sapiens
11 WNK2  
Affinity Capture-Western Homo sapiens
12 RRBP1 6238
Affinity Capture-MS Homo sapiens
13 ZFP36  
Affinity Capture-RNA Homo sapiens
14 PHLDB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EPDR1 54749
Affinity Capture-MS Homo sapiens
16 APPL1 26060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FBXO33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CCDC43 124808
Two-hybrid Homo sapiens
19 TRIM8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 WNK1 65125
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NAP1L2  
Two-hybrid Homo sapiens
22 UCKL1 54963
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GPRASP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 POLR3C 10623
Two-hybrid Homo sapiens
25 GIT2 9815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CLK2 1196
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 ACTC1 70
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
28 CDC37 11140
Two-hybrid Homo sapiens
29 NUDCD3 23386
Affinity Capture-MS Homo sapiens
30 TMA16  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 ACTR10 55860
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SCYL1 57410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MFAP1  
Two-hybrid Homo sapiens
35 UBE2C 11065
Two-hybrid Homo sapiens
36 DCTN2 10540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KLHL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
38 TRIM9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TXNDC12 51060
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 SAMD1  
Affinity Capture-MS Homo sapiens
41 NACC1 112939
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
42 PIK3C2B 5287
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ACTR1B 10120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ZNF114 163071
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 KLHL17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ACIN1 22985
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 APEX1 328
Affinity Capture-RNA Homo sapiens
49 Dctn2 69654
Affinity Capture-MS Mus musculus
50 DIABLO 56616
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 UBE2I 7329
Two-hybrid Homo sapiens
52 WNK3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 UPRT 139596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ZNF423  
Affinity Capture-MS Homo sapiens
55 TNPO2 30000
Two-hybrid Homo sapiens
56 YARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 FAM160B1 57700
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ALKBH4  
Affinity Capture-MS Homo sapiens
59 DCTN4 51164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 XIAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 DCTN5 84516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CASK 8573
Affinity Capture-MS Homo sapiens
63 BYSL 705
Two-hybrid Homo sapiens
64 DCTN1 1639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RNPS1 10921
Affinity Capture-MS Homo sapiens
66 TRIM50  
Two-hybrid Homo sapiens
67 CCDC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CUL7 9820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 C20orf85  
Two-hybrid Homo sapiens
70 ACTR1A 10121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 APBA1  
Affinity Capture-MS Homo sapiens
72 FBXW8 26259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 UCK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
74 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 KEAP1 9817
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 KLHL12  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
77 FBXL17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 DNAJC13 23317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 USP28 57646
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
80 KLHL2 11275
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
81 TRIM47 91107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 TCF25 22980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 UBE2D1 7321
Reconstituted Complex Homo sapiens
84 WDYHV1  
Two-hybrid Homo sapiens
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