Gene description for CLK2
Gene name CDC-like kinase 2
Gene symbol CLK2
Other names/aliases -
Species Homo sapiens
 Database cross references - CLK2
ExoCarta ExoCarta_1196
Vesiclepedia VP_1196
Entrez Gene 1196
HGNC 2069
MIM 602989
UniProt P49760  
 CLK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CLK2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine/tyrosine kinase activity GO:0004712 IEA
    protein tyrosine kinase activity GO:0004713 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    identical protein binding GO:0042802 IPI
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    response to ionizing radiation GO:0010212 IMP
    regulation of RNA splicing GO:0043484 IBA
    regulation of RNA splicing GO:0043484 IDA
    negative regulation of gluconeogenesis GO:0045721 ISS
    protein autophosphorylation GO:0046777 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nuclear body GO:0016604 IDA
    nuclear speck GO:0016607 IEA
 Experiment description of studies that identified CLK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CLK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 NUPL2 11097
Affinity Capture-MS Homo sapiens
3 RBMXL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LUZP4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 RSBN1L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 YTHDF1 54915
Two-hybrid Homo sapiens
8 PKP2 5318
Affinity Capture-MS Homo sapiens
9 PRPF38A 84950
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 ZC3H14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 SRSF11 9295
Affinity Capture-MS Homo sapiens
13 CPSF4  
Affinity Capture-MS Homo sapiens
14 HNRNPC 3183
Affinity Capture-MS Homo sapiens
15 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
16 ZNF398  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 PTPN1 5770
Biochemical Activity Homo sapiens
18 SRSF8 10929
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TACC2 10579
Two-hybrid Homo sapiens
20 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
21 Mrps21  
Two-hybrid Mus musculus
22 CPSF2 53981
Affinity Capture-MS Homo sapiens
23 SNRNP70 6625
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
24 RNPS1 10921
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 ZNF440  
Two-hybrid Homo sapiens
26 ZNF436  
Two-hybrid Homo sapiens
27 NFKBIL1  
Affinity Capture-MS Homo sapiens
28 RNF8  
Two-hybrid Homo sapiens
29 RSRP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TRIM50  
Two-hybrid Homo sapiens
31 Cep85l  
Affinity Capture-MS Mus musculus
32 NCOA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CPSF7 79869
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 ZSCAN21  
Two-hybrid Homo sapiens
35 SRRM2 23524
Affinity Capture-MS Homo sapiens
36 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PRPF4B 8899
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ZNF30  
Affinity Capture-MS Homo sapiens
39 SRSF3 6428
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
40 EED  
Affinity Capture-MS Homo sapiens
41 U2AF1 7307
Two-hybrid Homo sapiens
42 YWHAH 7533
Affinity Capture-MS Homo sapiens
43 ATG16L1 55054
Affinity Capture-MS Homo sapiens
44 MRPL4 51073
Two-hybrid Homo sapiens
45 SDCBP 6386
Two-hybrid Homo sapiens
46 LARP4B 23185
Affinity Capture-MS Homo sapiens
47 YWHAB 7529
Affinity Capture-MS Homo sapiens
48 PSME3 10197
Affinity Capture-MS Homo sapiens
49 EPB41L3 23136
Affinity Capture-MS Homo sapiens
50 ZNF394  
Two-hybrid Homo sapiens
51 CLK2 1196
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
52 SRRT 51593
Affinity Capture-MS Homo sapiens
53 SRPK2 6733
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 RBM6 10180
Two-hybrid Homo sapiens
55 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 RBM7  
Affinity Capture-MS Homo sapiens
57 ACIN1 22985
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SRSF6 6431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PURG  
Affinity Capture-MS Homo sapiens
60 FANCM 57697
Affinity Capture-Western Homo sapiens
61 UBE2I 7329
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 ZNF638 27332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PABPC1 26986
Affinity Capture-MS Homo sapiens
64 Rhoa 11848
Affinity Capture-MS Mus musculus
65 ZNF837  
Two-hybrid Homo sapiens
66 U2AF2 11338
Affinity Capture-MS Homo sapiens
67 RAD18  
Affinity Capture-MS Homo sapiens
68 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 HUWE1 10075
Affinity Capture-MS Homo sapiens
70 YWHAQ 10971
Affinity Capture-MS Homo sapiens
71 MB21D1  
Proximity Label-MS Homo sapiens
72 SNRNP27  
Two-hybrid Homo sapiens
73 FGFRL1 53834
Affinity Capture-MS Homo sapiens
74 ZRSR2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 Ube2i  
Two-hybrid Mus musculus
76 SON 6651
Affinity Capture-MS Homo sapiens
77 NKTR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 CDX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DAXX  
Affinity Capture-MS Homo sapiens
80 MRPL2 51069
Two-hybrid Homo sapiens
81 TRA2B 6434
Affinity Capture-MS Homo sapiens
82 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 BCLAF1 9774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PAIP2B  
Affinity Capture-MS Homo sapiens
85 RBBP6 5930
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 EPB41L1 2036
Affinity Capture-MS Homo sapiens
87 RBM39 9584
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
88 CPSF1 29894
Affinity Capture-MS Homo sapiens
89 ZNF473 25888
Two-hybrid Homo sapiens
90 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 SRSF7 6432
Affinity Capture-MS Homo sapiens
92 CLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
93 GPALPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 JPH4 84502
Affinity Capture-MS Homo sapiens
95 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SRSF4 6429
Affinity Capture-MS Homo sapiens
97 SRSF1 6426
Affinity Capture-MS Homo sapiens
98 WDR33 55339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 SETD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 TNPO3 23534
Affinity Capture-MS Homo sapiens
101 TRIM27  
Two-hybrid Homo sapiens
102 HNRNPF 3185
Affinity Capture-MS Homo sapiens
103 ZC3H11A  
Affinity Capture-MS Homo sapiens
104 UBL5 59286
Reconstituted Complex Homo sapiens
105 NIP7 51388
Two-hybrid Homo sapiens
106 NXF1 10482
Two-hybrid Homo sapiens
107 AP2M1 1173
Two-hybrid Homo sapiens
108 PPHLN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PRR3  
Affinity Capture-MS Homo sapiens
110 PATL1 219988
Two-hybrid Homo sapiens
111 ZNF558  
Two-hybrid Homo sapiens
112 ZNF491  
Two-hybrid Homo sapiens
113 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 PABPC5 140886
Affinity Capture-MS Homo sapiens
115 CLASRP  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 FIP1L1 81608
Affinity Capture-MS Homo sapiens
117 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
118 GPATCH8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 SRSF10 10772
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 CACTIN  
Two-hybrid Homo sapiens
121 ZNF136  
Two-hybrid Homo sapiens
122 GMCL1  
Two-hybrid Homo sapiens
123 TBC1D9B 23061
Affinity Capture-MS Homo sapiens
124 RBMS2 5939
Affinity Capture-MS Homo sapiens
125 ZNF263  
Two-hybrid Homo sapiens
126 KLHL2 11275
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
127 ECE1 1889
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
128 SRRM1 10250
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
129 CLK1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 RNF151  
Affinity Capture-MS Homo sapiens
131 CPSF6 11052
Affinity Capture-MS Homo sapiens
132 MDK 4192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 APOBEC3D  
Affinity Capture-MS Homo sapiens
134 ASPSCR1 79058
Two-hybrid Homo sapiens
135 YTHDC1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 EHHADH 1962
Two-hybrid Homo sapiens
137 SAP18 10284
Affinity Capture-MS Homo sapiens
138 SRSF12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 SNRPA 6626
Affinity Capture-MS Homo sapiens
140 HNRNPM 4670
Affinity Capture-MS Homo sapiens
141 THRAP3 9967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 ZNF764 92595
Two-hybrid Homo sapiens
143 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
144 EPB41L2 2037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 DDIT4L  
Two-hybrid Homo sapiens
146 DDX58 23586
Affinity Capture-RNA Homo sapiens
147 C19orf40  
Affinity Capture-Western Homo sapiens
148 ZNF317 57693
Two-hybrid Homo sapiens
149 SNIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
150 GSPT2 23708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 ZNF768 79724
Two-hybrid Homo sapiens
152 ZCCHC10  
Affinity Capture-MS Homo sapiens
153 PRPF6 24148
Two-hybrid Homo sapiens
154 PRM1  
Two-hybrid Homo sapiens
155 C17orf85  
Affinity Capture-MS Homo sapiens
156 GTPBP2 54676
Two-hybrid Homo sapiens
157 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
158 REPIN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 Rnps1  
Two-hybrid Mus musculus
160 LNX1  
Two-hybrid Homo sapiens
161 CDKN2A 1029
Affinity Capture-MS Homo sapiens
162 CASC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLK2 is involved
No pathways found





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