Gene description for EHHADH
Gene name enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Gene symbol EHHADH
Other names/aliases ECHD
FRTS3
L-PBE
LBFP
LBP
PBFE
Species Homo sapiens
 Database cross references - EHHADH
ExoCarta ExoCarta_1962
Vesiclepedia VP_1962
Entrez Gene 1962
HGNC 3247
MIM 607037
UniProt Q08426   Q9NY65  
 EHHADH identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Keratinocytes 19530224    
Keratinocytes 19530224    
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for EHHADH
Molecular Function
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IBA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IDA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISS
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 NAS
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 TAS
    delta(3)-delta(2)-enoyl-CoA isomerase activity GO:0004165 ISS
    enoyl-CoA hydratase activity GO:0004300 IDA
    enoyl-CoA hydratase activity GO:0004300 ISS
    enoyl-CoA hydratase activity GO:0004300 NAS
    protein binding GO:0005515 IPI
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 IDA
    intramolecular oxidoreductase activity, transposing C=C bonds GO:0016863 ISS
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 TAS
    enzyme binding GO:0019899 IPI
    NAD+ binding GO:0070403 IEA
Biological Process
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IDA
    fatty acid beta-oxidation GO:0006635 ISS
    fatty acid beta-oxidation GO:0006635 NAS
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 TAS
Subcellular Localization
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 ISS
    peroxisome GO:0005777 NAS
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified EHHADH in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EHHADH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Two-hybrid Homo sapiens
2 SGCB 6443
Two-hybrid Homo sapiens
3 SPATS2  
Affinity Capture-MS Homo sapiens
4 UTP18  
Affinity Capture-MS Homo sapiens
5 RBMXL1  
Affinity Capture-MS Homo sapiens
6 HSD17B11 51170
Two-hybrid Homo sapiens
7 TNIP1 10318
Two-hybrid Homo sapiens
8 KHDRBS3  
Affinity Capture-MS Homo sapiens
9 NECAB2  
Two-hybrid Homo sapiens
10 MAP9 79884
Affinity Capture-MS Homo sapiens
11 EMP1  
Two-hybrid Homo sapiens
12 REL 5966
Two-hybrid Homo sapiens
13 BOLL  
Two-hybrid Homo sapiens
14 ESF1 51575
Affinity Capture-MS Homo sapiens
15 ARMC12  
Two-hybrid Homo sapiens
16 GAS8  
Two-hybrid Homo sapiens
17 ZC3H4 23211
Affinity Capture-MS Homo sapiens
18 TRIM55  
Two-hybrid Homo sapiens
19 MID1 4281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 CCDC102B  
Two-hybrid Homo sapiens
21 TRIM54  
Two-hybrid Homo sapiens
22 USP36  
Affinity Capture-MS Homo sapiens
23 TCEB3 6924
Affinity Capture-MS Homo sapiens
24 CENPU  
Affinity Capture-MS Homo sapiens
25 SMN2 6607
Two-hybrid Homo sapiens
26 TMEM35  
Two-hybrid Homo sapiens
27 RPRM  
Two-hybrid Homo sapiens
28 CDK11B 984
Affinity Capture-MS Homo sapiens
29 PTGES 9536
Two-hybrid Homo sapiens
30 OPRM1 4988
Two-hybrid Homo sapiens
31 LSG1  
Affinity Capture-MS Homo sapiens
32 ARMCX3 51566
Two-hybrid Homo sapiens
33 MTUS2 23281
Two-hybrid Homo sapiens
34 TMEM17  
Two-hybrid Homo sapiens
35 ADARB1 104
Two-hybrid Homo sapiens
36 PRKRA 8575
Two-hybrid Homo sapiens
37 KRTAP4-11  
Two-hybrid Homo sapiens
38 DDX18 8886
Affinity Capture-MS Homo sapiens
39 LEPROTL1 23484
Two-hybrid Homo sapiens
40 DVL2 1856
Affinity Capture-MS Homo sapiens
41 KCTD6  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
42 LYPD5 284348
Two-hybrid Homo sapiens
43 LZTFL1 54585
Two-hybrid Homo sapiens
44 DAAM2 23500
Two-hybrid Homo sapiens
45 TRIM41 90933
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
46 CLK2 1196
Two-hybrid Homo sapiens
47 CLDN5 7122
Two-hybrid Homo sapiens
48 HTATIP2 10553
Two-hybrid Homo sapiens
49 UTP11L  
Affinity Capture-MS Homo sapiens
50 JAGN1 84522
Two-hybrid Homo sapiens
51 CDR2L  
Two-hybrid Homo sapiens
52 FUNDC1  
Two-hybrid Homo sapiens
53 PNMA5 114824
Two-hybrid Homo sapiens
54 MCM10 55388
Affinity Capture-MS Homo sapiens
55 SLC39A2  
Two-hybrid Homo sapiens
56 ZCCHC17  
Two-hybrid Homo sapiens
57 SAFB2 9667
Affinity Capture-MS Homo sapiens
58 C16orf58 64755
Two-hybrid Homo sapiens
59 TRIM21 6737
Two-hybrid Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 RRP36 88745
Affinity Capture-MS Homo sapiens
62 NSRP1  
Affinity Capture-MS Homo sapiens
63 CENPQ  
Affinity Capture-MS Homo sapiens
64 ACTB 60
Two-hybrid Homo sapiens
65 TPP2 7174
Two-hybrid Homo sapiens
66 SNRNP27  
Affinity Capture-MS Homo sapiens
67 AGPS 8540
Proximity Label-MS Homo sapiens
68 TMEM121  
Two-hybrid Homo sapiens
69 DNTTIP2  
Affinity Capture-MS Homo sapiens
70 ACTG1 71
Two-hybrid Homo sapiens
71 SYNGR3  
Two-hybrid Homo sapiens
72 TRIM63  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 PPP1R12C 54776
Two-hybrid Homo sapiens
74 SSX2IP  
Two-hybrid Homo sapiens
75 KRTAP10-7  
Two-hybrid Homo sapiens
76 ETHE1 23474
Co-fractionation Homo sapiens
77 CAT 847
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
78 HABP4  
Affinity Capture-MS Homo sapiens
79 MAB21L3  
Two-hybrid Homo sapiens
80 MAP7 9053
Affinity Capture-MS Homo sapiens
81 FGF14  
Two-hybrid Homo sapiens
82 KCTD9  
Two-hybrid Homo sapiens
83 SMIM5  
Two-hybrid Homo sapiens
84 SLC14A2  
Two-hybrid Homo sapiens
85 DES 1674
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
86 DCAF13 25879
Affinity Capture-MS Homo sapiens
87 BHLHE40  
Two-hybrid Homo sapiens
88 NACC1 112939
Two-hybrid Homo sapiens
89 ANKRD36B  
Two-hybrid Homo sapiens
90 NCDN 23154
Affinity Capture-MS Homo sapiens
91 ORMDL1 94101
Two-hybrid Homo sapiens
92 RP9  
Affinity Capture-MS Homo sapiens
93 LZTS2 84445
Two-hybrid Homo sapiens
94 PSTPIP1 9051
Two-hybrid Homo sapiens
95 SSNA1 8636
Two-hybrid Homo sapiens
96 SCARA5 286133
Two-hybrid Homo sapiens
97 PRRC2A 7916
Affinity Capture-MS Homo sapiens
98 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
99 HIP1 3092
Two-hybrid Homo sapiens
100 DIDO1  
Affinity Capture-MS Homo sapiens
101 TMEM31  
Two-hybrid Homo sapiens
102 TRIM5 85363
Two-hybrid Homo sapiens
103 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
104 HID1 283987
Two-hybrid Homo sapiens
105 SMN1 6606
Two-hybrid Homo sapiens
106 SDR16C5  
Two-hybrid Homo sapiens
107 VAC14 55697
Two-hybrid Homo sapiens
108 ZBTB8A  
Two-hybrid Homo sapiens
109 ZC3H13 23091
Affinity Capture-MS Homo sapiens
110 CYB561A3 220002
Two-hybrid Homo sapiens
111 ZBTB26  
Two-hybrid Homo sapiens
112 SLC7A6OS  
Affinity Capture-MS Homo sapiens
113 PNMA1 9240
Two-hybrid Homo sapiens
114 ZBTB10 65986
Two-hybrid Homo sapiens
115 PAQR8  
Two-hybrid Homo sapiens
116 ERGIC1 57222
Two-hybrid Homo sapiens
117 WBP11  
Affinity Capture-MS Homo sapiens
118 RIOK2 55781
Affinity Capture-MS Homo sapiens
119 AARS2  
Two-hybrid Homo sapiens
120 TRIM23 373
Two-hybrid Homo sapiens
121 ZBTB9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
122 CCDC13 152206
Two-hybrid Homo sapiens
123 DHRS4 10901
Affinity Capture-MS Homo sapiens
124 CISD2 493856
Two-hybrid Homo sapiens
125 PEX5 5830
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
126 LCN2 3934
Two-hybrid Homo sapiens
127 CEP83  
Two-hybrid Homo sapiens
128 TRAF1 7185
Two-hybrid Homo sapiens
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