Gene description for ORMDL1
Gene name ORMDL sphingolipid biosynthesis regulator 1
Gene symbol ORMDL1
Other names/aliases -
Species Homo sapiens
 Database cross references - ORMDL1
ExoCarta ExoCarta_94101
Vesiclepedia VP_94101
Entrez Gene 94101
HGNC 16036
MIM 610073
UniProt Q9P0S3  
 ORMDL1 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ORMDL1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    ceramide metabolic process GO:0006672 IBA
    ceramide metabolic process GO:0006672 IMP
    sphingomyelin biosynthetic process GO:0006686 IEA
    sphingolipid biosynthetic process GO:0030148 IBA
    myelination GO:0042552 IEA
    motor behavior GO:0061744 IEA
    intracellular sphingolipid homeostasis GO:0090156 IBA
    negative regulation of ceramide biosynthetic process GO:1900060 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    serine palmitoyltransferase complex GO:0017059 IBA
 Experiment description of studies that identified ORMDL1 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ORMDL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM35  
Two-hybrid Homo sapiens
2 MAGEA8  
Affinity Capture-MS Homo sapiens
3 ERGIC3 51614
Two-hybrid Homo sapiens
4 TMEM143  
Two-hybrid Homo sapiens
5 GGACT 87769
Affinity Capture-MS Homo sapiens
6 SLC30A8  
Two-hybrid Homo sapiens
7 SLC22A8 9376
Affinity Capture-MS Homo sapiens
8 PTH1R  
Affinity Capture-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 TTC33  
Affinity Capture-MS Homo sapiens
11 EBAG9 9166
Two-hybrid Homo sapiens
12 TMEM63B 55362
Affinity Capture-MS Homo sapiens
13 ZFYVE27 118813
Two-hybrid Homo sapiens
14 TMEM52B 120939
Two-hybrid Homo sapiens
15 EHHADH 1962
Two-hybrid Homo sapiens
16 ASGR2  
Two-hybrid Homo sapiens
17 CRELD1 78987
Affinity Capture-MS Homo sapiens
18 TMEM14B  
Two-hybrid Homo sapiens
19 SPTLC1 10558
Affinity Capture-MS Homo sapiens
20 GPR12  
Affinity Capture-MS Homo sapiens
21 FPR1  
Affinity Capture-MS Homo sapiens
22 HSD17B11 51170
Two-hybrid Homo sapiens
23 SLC5A6 8884
Affinity Capture-MS Homo sapiens
24 EBP  
Two-hybrid Homo sapiens
25 KCNK4  
Two-hybrid Homo sapiens
26 AQP3  
Affinity Capture-MS Homo sapiens
27 FAM83H 286077
Affinity Capture-MS Homo sapiens
28 SLC22A9  
Affinity Capture-MS Homo sapiens
29 APP 351
Reconstituted Complex Homo sapiens
30 STOM 2040
Two-hybrid Homo sapiens
31 GPR152  
Two-hybrid Homo sapiens
32 DCUN1D5 84259
Affinity Capture-MS Homo sapiens
33 CPLX4  
Two-hybrid Homo sapiens
34 SLC10A1  
Two-hybrid Homo sapiens
35 CREB3L1  
Two-hybrid Homo sapiens
36 CREB3  
Two-hybrid Homo sapiens
37 SLC10A6  
Two-hybrid Homo sapiens
38 VMA21  
Two-hybrid Homo sapiens
39 PCNXL2  
Two-hybrid Homo sapiens
40 ARL13B 200894
Two-hybrid Homo sapiens
41 SLC22A4 6583
Affinity Capture-MS Homo sapiens
42 NEMF 9147
Affinity Capture-MS Homo sapiens
43 SCN3B  
Two-hybrid Homo sapiens
44 PGRMC2 10424
Two-hybrid Homo sapiens
45 ZDHHC15 158866
Two-hybrid Homo sapiens
46 SLC2A9  
Affinity Capture-MS Homo sapiens
47 HIATL1 84641
Two-hybrid Homo sapiens
48 TMEM56  
Two-hybrid Homo sapiens
49 Kif2a 16563
Affinity Capture-MS Mus musculus
50 REEP4  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ORMDL1 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





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