Gene description for NEMF
Gene name nuclear export mediator factor
Gene symbol NEMF
Other names/aliases NY-CO-1
SDCCAG1
Species Homo sapiens
 Database cross references - NEMF
ExoCarta ExoCarta_9147
Vesiclepedia VP_9147
Entrez Gene 9147
HGNC 10663
MIM 608378
UniProt O60524  
 NEMF identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for NEMF
Molecular Function
    tRNA binding GO:0000049 IBA
    ribosomal large subunit binding GO:0043023 IBA
    ribosomal large subunit binding GO:0043023 IDA
    alpha-aminoacyl-tRNA binding GO:1904678 IDA
Biological Process
    nuclear export GO:0051168 IMP
    protein-containing complex assembly GO:0065003 IMP
    rescue of stalled ribosome GO:0072344 IBA
    rescue of stalled ribosome GO:0072344 IDA
    CAT tailing GO:0140708 IDA
    ribosome-associated ubiquitin-dependent protein catabolic process GO:1990116 IBA
    ribosome-associated ubiquitin-dependent protein catabolic process GO:1990116 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IDA
    cytosolic ribosome GO:0022626 IDA
    RQC complex GO:1990112 IBA
    RQC complex GO:1990112 IDA
 Experiment description of studies that identified NEMF in exosomes
1
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NEMF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
2 DDX18 8886
Co-fractionation Homo sapiens
3 ZBTB38 253461
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HYPK 25764
Affinity Capture-MS Homo sapiens
5 DHX15 1665
Co-fractionation Homo sapiens
6 ZRANB1 54764
Affinity Capture-MS Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 CYP4V2  
Affinity Capture-MS Homo sapiens
10 SMC1A 8243
Co-fractionation Homo sapiens
11 ORMDL1 94101
Affinity Capture-MS Homo sapiens
12 ANKRD18A  
Affinity Capture-MS Homo sapiens
13 SPANXN2  
Affinity Capture-MS Homo sapiens
14 FBXL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 FADS3 3995
Affinity Capture-MS Homo sapiens
16 POLR1B 84172
Co-fractionation Homo sapiens
17 TCEAL7  
Affinity Capture-MS Homo sapiens
18 PPM1E  
Co-fractionation Homo sapiens
19 PSENEN  
Affinity Capture-MS Homo sapiens
20 GYPA  
Affinity Capture-MS Homo sapiens
21 CCDC132 55610
Co-fractionation Homo sapiens
22 NABP2  
Co-fractionation Homo sapiens
23 ZNF622 90441
Affinity Capture-MS Homo sapiens
24 COPB1 1315
Co-fractionation Homo sapiens
25 EZR 7430
Affinity Capture-MS Homo sapiens
26 HAUS2  
Affinity Capture-MS Homo sapiens
27 Ccdc77  
Affinity Capture-MS Mus musculus
28 NOP56 10528
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
29 COX5A 9377
Co-fractionation Homo sapiens
30 RPL10 6134
Affinity Capture-MS Homo sapiens
31 RPS7 6201
Cross-Linking-MS (XL-MS) Homo sapiens
32 GNL2 29889
Affinity Capture-MS Homo sapiens
33 THOC5 8563
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 MCM4 4173
Co-fractionation Homo sapiens
36 ZNF490  
Affinity Capture-MS Homo sapiens
37 EIF2S2 8894
Co-fractionation Homo sapiens
38 HSP90B1 7184
Co-fractionation Homo sapiens
39 RPL19 6143
Affinity Capture-MS Homo sapiens
40 MMGT1 93380
Affinity Capture-MS Homo sapiens
41 UFL1 23376
Affinity Capture-MS Homo sapiens
42 DIMT1 27292
Affinity Capture-MS Homo sapiens
43 RPL5 6125
Affinity Capture-MS Homo sapiens
44 AKR7L 246181
Affinity Capture-MS Homo sapiens
45 Tipin  
Affinity Capture-MS Mus musculus
46 SPCS1 28972
Affinity Capture-MS Homo sapiens
47 Sept6  
Affinity Capture-MS Mus musculus
48 HECTD1 25831
Affinity Capture-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 Rpl35 66489
Affinity Capture-MS Mus musculus
51 COPE 11316
Co-fractionation Homo sapiens
52 KXD1 79036
Affinity Capture-MS Homo sapiens
53 STK11 6794
Affinity Capture-MS Homo sapiens
54 FBXW7  
Affinity Capture-MS Homo sapiens
55 COPA 1314
Co-fractionation Homo sapiens
56 CERS6  
Affinity Capture-MS Homo sapiens
57 DDRGK1 65992
Affinity Capture-MS Homo sapiens
58 LTN1 26046
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
59 XRCC3  
Affinity Capture-MS Homo sapiens
60 OBSL1 23363
Affinity Capture-MS Homo sapiens
61 ZNF786  
Affinity Capture-MS Homo sapiens
62 CC2D1B 200014
Co-fractionation Homo sapiens
63 KLK12  
Affinity Capture-MS Homo sapiens
64 SEC61B 10952
Affinity Capture-Western Homo sapiens
65 EP300 2033
Affinity Capture-MS Homo sapiens
66 RPS16 6217
Affinity Capture-MS Homo sapiens
67 C1orf189  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NEMF is involved
No pathways found





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