Gene description for AKR7L
Gene name aldo-keto reductase family 7-like (gene/pseudogene)
Gene symbol AKR7L
Other names/aliases AFAR3
AFB1-AR3
AKR7A4
Species Homo sapiens
 Database cross references - AKR7L
ExoCarta ExoCarta_246181
Vesiclepedia VP_246181
Entrez Gene 246181
HGNC 24056
MIM 608478
 AKR7L identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for AKR7L
Molecular Function
    aldo-keto reductase (NADPH) activity GO:0004033 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified AKR7L in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AKR7L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Affinity Capture-MS Homo sapiens
2 DDX46 9879
Affinity Capture-MS Homo sapiens
3 GCFC2  
Affinity Capture-MS Homo sapiens
4 MARK3 4140
Affinity Capture-MS Homo sapiens
5 DST 667
Affinity Capture-MS Homo sapiens
6 GTF2F1 2962
Affinity Capture-MS Homo sapiens
7 PEF1 553115
Affinity Capture-MS Homo sapiens
8 KIF2A 3796
Affinity Capture-MS Homo sapiens
9 NXT2  
Affinity Capture-MS Homo sapiens
10 RIOK1 83732
Affinity Capture-MS Homo sapiens
11 GIGYF2 26058
Affinity Capture-MS Homo sapiens
12 C12orf43  
Affinity Capture-MS Homo sapiens
13 SPATS2L 26010
Affinity Capture-MS Homo sapiens
14 FBXW5  
Affinity Capture-MS Homo sapiens
15 ZRANB2 9406
Affinity Capture-MS Homo sapiens
16 DHX36 170506
Affinity Capture-MS Homo sapiens
17 CLASP2 23122
Affinity Capture-MS Homo sapiens
18 MACF1 23499
Affinity Capture-MS Homo sapiens
19 YTHDF1 54915
Affinity Capture-MS Homo sapiens
20 PPIL4  
Affinity Capture-MS Homo sapiens
21 PINX1  
Affinity Capture-MS Homo sapiens
22 PARD3 56288
Affinity Capture-MS Homo sapiens
23 RBMXL1  
Affinity Capture-MS Homo sapiens
24 DGCR8  
Affinity Capture-MS Homo sapiens
25 ESF1 51575
Affinity Capture-MS Homo sapiens
26 SCYL1 57410
Affinity Capture-MS Homo sapiens
27 PNISR  
Affinity Capture-MS Homo sapiens
28 RFC5 5985
Affinity Capture-MS Homo sapiens
29 PNMAL1  
Affinity Capture-MS Homo sapiens
30 UBR3  
Affinity Capture-MS Homo sapiens
31 RBM6 10180
Affinity Capture-MS Homo sapiens
32 UPF2 26019
Affinity Capture-MS Homo sapiens
33 DDX47 51202
Affinity Capture-MS Homo sapiens
34 CCDC97  
Affinity Capture-MS Homo sapiens
35 PDCD11 22984
Affinity Capture-MS Homo sapiens
36 SPATS2  
Affinity Capture-MS Homo sapiens
37 RPP38 10557
Affinity Capture-MS Homo sapiens
38 RFC1 5981
Affinity Capture-MS Homo sapiens
39 DHX40  
Affinity Capture-MS Homo sapiens
40 TCEB3 6924
Affinity Capture-MS Homo sapiens
41 MCM10 55388
Affinity Capture-MS Homo sapiens
42 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
43 MARK2 2011
Affinity Capture-MS Homo sapiens
44 RIOK2 55781
Affinity Capture-MS Homo sapiens
45 RPP25  
Affinity Capture-MS Homo sapiens
46 HEXIM1 10614
Affinity Capture-MS Homo sapiens
47 DHX38 9785
Affinity Capture-MS Homo sapiens
48 GTF2F2 2963
Affinity Capture-MS Homo sapiens
49 TRIP4 9325
Affinity Capture-MS Homo sapiens
50 RPP40 10799
Affinity Capture-MS Homo sapiens
51 MAP9 79884
Affinity Capture-MS Homo sapiens
52 DHX16 8449
Affinity Capture-MS Homo sapiens
53 FOCAD 54914
Affinity Capture-MS Homo sapiens
54 SRPK1 6732
Affinity Capture-MS Homo sapiens
55 AKR7A2 8574
Affinity Capture-MS Homo sapiens
56 SRFBP1  
Affinity Capture-MS Homo sapiens
57 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
58 UPF3B 65109
Affinity Capture-MS Homo sapiens
59 MAP1A 4130
Affinity Capture-MS Homo sapiens
60 AKR7A3 22977
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PHF8  
Affinity Capture-MS Homo sapiens
62 NEMF 9147
Affinity Capture-MS Homo sapiens
63 STRBP 55342
Affinity Capture-MS Homo sapiens
64 NVL  
Affinity Capture-MS Homo sapiens
65 GPKOW  
Affinity Capture-MS Homo sapiens
66 DDX49 54555
Affinity Capture-MS Homo sapiens
67 DDX55  
Affinity Capture-MS Homo sapiens
68 SRRT 51593
Affinity Capture-MS Homo sapiens
69 FAM117A 81558
Affinity Capture-MS Homo sapiens
70 HABP4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AKR7L is involved
PathwayEvidenceSource
Aflatoxin activation and detoxification TAS Reactome
Biological oxidations TAS Reactome
Metabolism TAS Reactome





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