Gene description for TCEB3
Gene name transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Gene symbol TCEB3
Other names/aliases EloA
SIII
SIII p110
TCEB3A
Species Homo sapiens
 Database cross references - TCEB3
ExoCarta ExoCarta_6924
Vesiclepedia VP_6924
Entrez Gene 6924
HGNC 11620
MIM 600786
UniProt Q14241  
 TCEB3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TCEB3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 TAS
    transcription initiation at RNA polymerase II promoter GO:0006367 IDA
    transcription elongation by RNA polymerase II GO:0006368 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    nucleoplasm GO:0005654 TAS
    elongin complex GO:0070449 IDA
    site of DNA damage GO:0090734 ISS
 Experiment description of studies that identified TCEB3 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TCEB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 CD3EAP  
Proximity Label-MS Homo sapiens
3 CDC14B 8555
Proximity Label-MS Homo sapiens
4 H2AFJ 55766
Affinity Capture-MS Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 AFF4 27125
Affinity Capture-MS Homo sapiens
7 POLR2G 5436
Co-localization Homo sapiens
Co-localization Homo sapiens
8 TCEB1 6921
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 COIL  
Proximity Label-MS Homo sapiens
10 DLST 1743
Co-fractionation Homo sapiens
11 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Co-localization Homo sapiens
13 SPERT  
Two-hybrid Homo sapiens
14 ANAPC15  
Affinity Capture-MS Homo sapiens
15 FAM9B  
Two-hybrid Homo sapiens
16 NOP56 10528
Proximity Label-MS Homo sapiens
17 DHX8 1659
Proximity Label-MS Homo sapiens
18 INTS2  
Affinity Capture-MS Homo sapiens
19 MDFI  
Two-hybrid Homo sapiens
20 C9orf78 51759
Affinity Capture-MS Homo sapiens
21 SOCS1  
Affinity Capture-Western Homo sapiens
22 PRPF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 VHL  
Affinity Capture-Western Homo sapiens
24 INTS1 26173
Affinity Capture-MS Homo sapiens
25 PPP1R10  
Affinity Capture-MS Homo sapiens
26 SOX2  
Affinity Capture-MS Homo sapiens
27 TRIM54  
Two-hybrid Homo sapiens
28 RNF40 9810
Co-fractionation Homo sapiens
29 APEX1 328
Proximity Label-MS Homo sapiens
30 DDX23 9416
Proximity Label-MS Homo sapiens
31 CEP70  
Two-hybrid Homo sapiens
32 CHAF1A  
Co-fractionation Homo sapiens
33 CEP57L1  
Two-hybrid Homo sapiens
34 KRT40  
Two-hybrid Homo sapiens
35 SLC13A2 9058
Affinity Capture-MS Homo sapiens
36 POLR2I 5438
Co-localization Homo sapiens
Co-localization Homo sapiens
37 PLK4  
Two-hybrid Homo sapiens
38 CDK12 51755
Co-fractionation Homo sapiens
39 AKR7L 246181
Affinity Capture-MS Homo sapiens
40 COL8A1 1295
Affinity Capture-MS Homo sapiens
41 DDRGK1 65992
Affinity Capture-MS Homo sapiens
42 POLR1E  
Proximity Label-MS Homo sapiens
43 ASB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 PEA15 8682
Affinity Capture-MS Homo sapiens
45 MID2 11043
Two-hybrid Homo sapiens
46 PSMB9 5698
Co-localization Homo sapiens
Co-localization Homo sapiens
47 POLR2C 5432
Co-localization Homo sapiens
Co-localization Homo sapiens
48 FBL 2091
Proximity Label-MS Homo sapiens
49 MECP2 4204
Affinity Capture-MS Homo sapiens
50 INTS12  
Affinity Capture-MS Homo sapiens
51 TACO1  
Affinity Capture-MS Homo sapiens
52 PES1 23481
Affinity Capture-MS Homo sapiens
53 NINL  
Two-hybrid Homo sapiens
54 POLR2K  
Co-localization Homo sapiens
Co-localization Homo sapiens
55 PRPF6 24148
Affinity Capture-MS Homo sapiens
56 TCP1 6950
Cross-Linking-MS (XL-MS) Homo sapiens
57 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
58 TMEM14B  
Affinity Capture-MS Homo sapiens
59 SRRM1 10250
Affinity Capture-MS Homo sapiens
60 SUPT5H 6829
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
61 IFI27L1  
Affinity Capture-MS Homo sapiens
62 CBX3 11335
Proximity Label-MS Homo sapiens
63 NRXN1  
Affinity Capture-MS Homo sapiens
64 WDR61 80349
Affinity Capture-MS Homo sapiens
65 SUPT16H 11198
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
66 C12orf49  
Negative Genetic Homo sapiens
67 SREBF2 6721
Negative Genetic Homo sapiens
68 C16orf58 64755
Affinity Capture-MS Homo sapiens
69 BARD1 580
Affinity Capture-MS Homo sapiens
70 NTRK1 4914
Affinity Capture-MS Homo sapiens
71 RPL31 6160
Proximity Label-MS Homo sapiens
72 IFI16 3428
Affinity Capture-MS Homo sapiens
73 INTS7  
Affinity Capture-MS Homo sapiens
74 RAP1A 5906
Reconstituted Complex Homo sapiens
75 FBXL6  
Affinity Capture-MS Homo sapiens
76 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
77 CSNK1E 1454
Affinity Capture-MS Homo sapiens
78 HECTD1 25831
Affinity Capture-MS Homo sapiens
79 CTR9 9646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
80 REXO1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
81 BRCA1 672
Two-hybrid Homo sapiens
82 C1orf189  
Affinity Capture-MS Homo sapiens
83 POLR2B 5431
Co-localization Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
84 ANTXR2 118429
Affinity Capture-MS Homo sapiens
85 OGT 8473
Reconstituted Complex Homo sapiens
86 LEO1 123169
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 CDC73  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
88 POLR2H 5437
Co-localization Homo sapiens
Co-localization Homo sapiens
89 TCEAL7  
Affinity Capture-MS Homo sapiens
90 PSENEN  
Affinity Capture-MS Homo sapiens
91 ECT2 1894
Affinity Capture-MS Homo sapiens
92 POLR2E 5434
Co-localization Homo sapiens
Co-localization Homo sapiens
93 SIRPA 140885
Reconstituted Complex Homo sapiens
94 CUL3 8452
Affinity Capture-MS Homo sapiens
95 YWHAZ 7534
Co-localization Homo sapiens
96 CTTN 2017
Affinity Capture-MS Homo sapiens
97 POLR2D  
Co-localization Homo sapiens
Co-localization Homo sapiens
98 KRTAP10-3  
Two-hybrid Homo sapiens
99 POLR2A 5430
Co-localization Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
100 SART1 9092
Affinity Capture-MS Homo sapiens
101 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
103 LBH  
Affinity Capture-MS Homo sapiens
104 FAM134B 54463
Cross-Linking-MS (XL-MS) Homo sapiens
105 LRPAP1 4043
Affinity Capture-MS Homo sapiens
106 PRPF19 27339
Affinity Capture-MS Homo sapiens
107 MYC  
Affinity Capture-MS Homo sapiens
108 RPA3 6119
Proximity Label-MS Homo sapiens
109 PHF6  
Affinity Capture-MS Homo sapiens
110 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
111 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
112 TCEB2 6923
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 PARP1 142
Proximity Label-MS Homo sapiens
114 RHOB 388
Proximity Label-MS Homo sapiens
115 LYAR 55646
Affinity Capture-MS Homo sapiens
116 GMCL1  
Two-hybrid Homo sapiens
117 NFIC 4782
Co-fractionation Homo sapiens
118 MGEA5 10724
Co-fractionation Homo sapiens
119 U2SURP 23350
Affinity Capture-MS Homo sapiens
120 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
121 FASN 2194
Positive Genetic Homo sapiens
122 POLR2F  
Co-localization Homo sapiens
Co-localization Homo sapiens
123 KAT8  
Co-localization Homo sapiens
124 POLR2J  
Co-localization Homo sapiens
Co-localization Homo sapiens
125 HOMEZ  
Two-hybrid Homo sapiens
126 RPS21 6227
Affinity Capture-MS Homo sapiens
127 C9orf114  
Affinity Capture-MS Homo sapiens
128 POLR2L 5441
Co-localization Homo sapiens
Co-localization Homo sapiens
129 USP36  
Affinity Capture-MS Homo sapiens
130 EHHADH 1962
Affinity Capture-MS Homo sapiens
131 CUL5 8065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
FRET Homo sapiens
132 KNOP1 400506
Affinity Capture-MS Homo sapiens
133 INTS4  
Affinity Capture-MS Homo sapiens
134 EZH1  
Affinity Capture-MS Homo sapiens
135 NIFK 84365
Proximity Label-MS Homo sapiens
136 DHX40  
Proximity Label-MS Homo sapiens
137 C20orf197  
Affinity Capture-MS Homo sapiens
138 RPS24 6229
Proximity Label-MS Homo sapiens
139 SPCS1 28972
Affinity Capture-MS Homo sapiens
140 SUPT6H 6830
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
141 JAKMIP2  
Two-hybrid Homo sapiens
142 RPS6 6194
Proximity Label-MS Homo sapiens
143 GAGE5  
Affinity Capture-MS Homo sapiens
144 PRPF4 9128
Affinity Capture-MS Homo sapiens
145 CYCS 54205
Affinity Capture-MS Homo sapiens
146 PAF1 54623
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
147 FAM178B 51252
Cross-Linking-MS (XL-MS) Homo sapiens
148 SSRP1 6749
Affinity Capture-MS Homo sapiens
149 NEDD8 4738
Affinity Capture-MS Homo sapiens
150 PARN  
Co-fractionation Homo sapiens
View the network image/svg+xml



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