Gene description for TCEB3
Gene name transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Gene symbol TCEB3
Other names/aliases EloA
SIII
SIII p110
TCEB3A
Species Homo sapiens
 Database cross references - TCEB3
ExoCarta ExoCarta_6924
Entrez Gene 6924
HGNC 11620
MIM 600786
UniProt Q14241  
 TCEB3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TCEB3
Molecular Function
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    ubiquitin-protein transferase activity GO:0004842 IBA
Biological Process
    viral process GO:0016032 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    gene expression GO:0010467 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    positive regulation of viral transcription GO:0050434 TAS
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
Subcellular Localization
    nucleus GO:0005634 IDA
    integral component of membrane GO:0016021 IEA
    elongin complex GO:0070449 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified TCEB3 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TCEB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SOCS1  
Affinity Capture-Western Homo sapiens
2 TCEB1 6921
Invitro Homo sapiens
3 TCEB2 6923
Invitro Homo sapiens
4 KIAA0980  
Two-hybrid Homo sapiens
View the network image/svg+xml



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