Gene description for NFIC
Gene name nuclear factor I/C (CCAAT-binding transcription factor)
Gene symbol NFIC
Other names/aliases CTF
CTF5
NF-I
NFI
Species Homo sapiens
 Database cross references - NFIC
ExoCarta ExoCarta_4782
Vesiclepedia VP_4782
Entrez Gene 4782
HGNC 7786
MIM 600729
UniProt P08651  
 NFIC identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NFIC
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    DNA replication GO:0006260 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    chromatin GO:0000785 ISA
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified NFIC in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NFIC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FOXC1  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
2 NCBP1 4686
Affinity Capture-MS Homo sapiens
3 PRDM1  
Reconstituted Complex Homo sapiens
4 MC1R 4157
Affinity Capture-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 FEV  
Proximity Label-MS Homo sapiens
7 NFIB  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
9 POGZ 23126
Proximity Label-MS Homo sapiens
10 MORC4  
Proximity Label-MS Homo sapiens
11 GATA2  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 ZNF608  
Proximity Label-MS Homo sapiens
13 PAX2  
Reconstituted Complex Homo sapiens
14 TCF7L2  
Proximity Label-MS Homo sapiens
15 RNMT 8731
Co-fractionation Homo sapiens
16 TLE3  
Proximity Label-MS Homo sapiens
17 KDM6A  
Proximity Label-MS Homo sapiens
18 CAMSAP2  
Co-fractionation Homo sapiens
19 ARID3B  
Proximity Label-MS Homo sapiens
20 FBRS 64319
Proximity Label-MS Homo sapiens
21 SMARCD2 6603
Proximity Label-MS Homo sapiens
22 SOX10  
Reconstituted Complex Homo sapiens
23 SOX2  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 MNX1  
Proximity Label-MS Homo sapiens
25 PAX6  
Reconstituted Complex Homo sapiens
26 SATB2  
Proximity Label-MS Homo sapiens
27 APEX1 328
Affinity Capture-RNA Homo sapiens
28 TCEB3 6924
Co-fractionation Homo sapiens
29 NCOR1  
Proximity Label-MS Homo sapiens
30 VPS72  
Proximity Label-MS Homo sapiens
31 GSE1 23199
Proximity Label-MS Homo sapiens
32 KLF8  
Reconstituted Complex Homo sapiens
33 FBRSL1  
Proximity Label-MS Homo sapiens
34 DNAJC3 5611
Co-fractionation Homo sapiens
35 TBL1XR1 79718
Proximity Label-MS Homo sapiens
36 NR2C2  
Proximity Label-MS Homo sapiens
37 E2F6 1876
Proximity Label-MS Homo sapiens
38 NUP35 129401
Proximity Label-MS Homo sapiens
39 ARID4B  
Proximity Label-MS Homo sapiens
40 CUX1 1523
Proximity Label-MS Homo sapiens
41 KMT2D 8085
Proximity Label-MS Homo sapiens
42 NCOA3  
Proximity Label-MS Homo sapiens
43 TEAD1  
Proximity Label-MS Homo sapiens
44 ZNF644  
Proximity Label-MS Homo sapiens
45 HMG20A  
Proximity Label-MS Homo sapiens
46 BCOR  
Proximity Label-MS Homo sapiens
47 NR2C1  
Proximity Label-MS Homo sapiens
48 RLF 6018
Proximity Label-MS Homo sapiens
49 WIZ 58525
Proximity Label-MS Homo sapiens
50 PLCB3 5331
Co-fractionation Homo sapiens
51 SMARCA2 6595
Proximity Label-MS Homo sapiens
52 RCOR3  
Proximity Label-MS Homo sapiens
53 RBPJ 3516
Affinity Capture-MS Homo sapiens
54 KIF14 9928
Affinity Capture-MS Homo sapiens
55 Cebpb  
Affinity Capture-Western Rattus norvegicus
56 FOXP1 27086
Proximity Label-MS Homo sapiens
57 KLF16  
Reconstituted Complex Homo sapiens
58 ZHX1  
Proximity Label-MS Homo sapiens
59 NSUN2 54888
Co-fractionation Homo sapiens
60 FOXA1  
Affinity Capture-MS Homo sapiens
61 FOXP4  
Proximity Label-MS Homo sapiens
62 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
63 TLX3  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
64 RFX1  
Affinity Capture-Western Homo sapiens
65 RUFY1 80230
Co-fractionation Homo sapiens
66 TET2  
Proximity Label-MS Homo sapiens
67 NCOR2  
Proximity Label-MS Homo sapiens
68 HIVEP1 3096
Proximity Label-MS Homo sapiens
69 TBR1  
Reconstituted Complex Homo sapiens
70 ELK3  
Reconstituted Complex Homo sapiens
71 NFIX 4784
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TRIM24  
Proximity Label-MS Homo sapiens
73 TKTL2  
Two-hybrid Homo sapiens
74 TFDP1  
Proximity Label-MS Homo sapiens
75 ZEB2  
Proximity Label-MS Homo sapiens
76 NFIA 4774
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 LHX2  
Reconstituted Complex Homo sapiens
78 GATA3  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
79 DPF2  
Proximity Label-MS Homo sapiens
80 SMAD3 4088
Affinity Capture-Western Homo sapiens
81 LHX3  
Reconstituted Complex Homo sapiens
82 SIX4  
Proximity Label-MS Homo sapiens
83 PAX8  
Reconstituted Complex Homo sapiens
84 CIC 23152
Proximity Label-MS Homo sapiens
85 ZKSCAN7  
Two-hybrid Homo sapiens
86 CTR9 9646
Affinity Capture-MS Homo sapiens
87 T  
Reconstituted Complex Homo sapiens
88 L3MBTL2  
Proximity Label-MS Homo sapiens
89 CEBPA  
Proximity Label-MS Homo sapiens
90 SOX17  
Reconstituted Complex Homo sapiens
91 ZNF609  
Proximity Label-MS Homo sapiens
92 GPS2  
Proximity Label-MS Homo sapiens
93 BCL7C  
Proximity Label-MS Homo sapiens
94 ZMYM4  
Proximity Label-MS Homo sapiens
95 ATXN1 6310
Proximity Label-MS Homo sapiens
96 BIRC2  
Affinity Capture-MS Homo sapiens
97 TLX2  
Reconstituted Complex Homo sapiens
98 RNF40 9810
Co-fractionation Homo sapiens
99 SFN 2810
Reconstituted Complex Homo sapiens
100 PAXIP1  
Proximity Label-MS Homo sapiens
101 SOX6  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
102 SMARCD1 6602
Proximity Label-MS Homo sapiens
103 MGA  
Proximity Label-MS Homo sapiens
104 EFTUD1 79631
Co-fractionation Homo sapiens
105 RLIM 51132
Affinity Capture-MS Homo sapiens
106 LHX4  
Reconstituted Complex Homo sapiens
107 TFAP4 7023
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
108 SSB 6741
Co-fractionation Homo sapiens
109 DTX2 113878
Proximity Label-MS Homo sapiens
110 YY1 7528
Reconstituted Complex Homo sapiens
111 FOXL1  
Reconstituted Complex Homo sapiens
112 BRMS1L  
Proximity Label-MS Homo sapiens
113 SEC24A 10802
Co-fractionation Homo sapiens
114 ELMSAN1  
Proximity Label-MS Homo sapiens
115 ARID1B 57492
Proximity Label-MS Homo sapiens
116 FOS 2353
Reconstituted Complex Homo sapiens
117 ARID3A  
Proximity Label-MS Homo sapiens
118 CSE1L 1434
Co-fractionation Homo sapiens
119 EHMT2 10919
Proximity Label-MS Homo sapiens
120 RREB1 6239
Proximity Label-MS Homo sapiens
121 TBL1X 6907
Proximity Label-MS Homo sapiens
122 PCGF1 84759
Proximity Label-MS Homo sapiens
123 SOX5 6660
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
124 ARID1A 8289
Proximity Label-MS Homo sapiens
125 SOX9  
Reconstituted Complex Homo sapiens
126 LMNA 4000
Co-fractionation Homo sapiens
127 FHL2 2274
Affinity Capture-MS Homo sapiens
128 ZCCHC14 23174
Two-hybrid Homo sapiens
129 TLX1  
Proximity Label-MS Homo sapiens
130 PARP1 142
Affinity Capture-MS Homo sapiens
131 ROCK1 6093
Co-fractionation Homo sapiens
132 TRPS1  
Proximity Label-MS Homo sapiens
133 SAP130  
Proximity Label-MS Homo sapiens
134 HOMEZ  
Proximity Label-MS Homo sapiens
135 SOX15  
Reconstituted Complex Homo sapiens
136 ATXN1L  
Proximity Label-MS Homo sapiens
137 LONP1 9361
Co-fractionation Homo sapiens
138 MEN1 4221
Affinity Capture-MS Homo sapiens
139 DPY30 84661
Proximity Label-MS Homo sapiens
140 TLE1 7088
Proximity Label-MS Homo sapiens
141 SMURF1 57154
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
142 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
143 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
144 PAX9  
Proximity Label-MS Homo sapiens
145 CREB1  
Affinity Capture-MS Homo sapiens
146 MKI67  
Affinity Capture-MS Homo sapiens
147 PRR12 57479
Proximity Label-MS Homo sapiens
148 HNRNPK 3190
Co-fractionation Homo sapiens
149 SSBP1 6742
Proximity Label-MS Homo sapiens
150 POU2F1 5451
Proximity Label-MS Homo sapiens
151 ETV7  
Reconstituted Complex Homo sapiens
152 PAX7  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
153 ZNF148  
Proximity Label-MS Homo sapiens
154 ZNF281  
Proximity Label-MS Homo sapiens
155 ERG  
Reconstituted Complex Homo sapiens
156 EHMT1  
Proximity Label-MS Homo sapiens
157 ELF2  
Reconstituted Complex Homo sapiens
158 KDM2B 84678
Proximity Label-MS Homo sapiens
159 AR 367
Proximity Label-MS Homo sapiens
160 TPD52L3  
Two-hybrid Homo sapiens
161 FOXE1  
Affinity Capture-MS Homo sapiens
162 SP7  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
163 KLF3  
Reconstituted Complex Homo sapiens
164 LLPH  
Two-hybrid Homo sapiens
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