Gene description for RBPJ
Gene name recombination signal binding protein for immunoglobulin kappa J region
Gene symbol RBPJ
Other names/aliases AOS3
CBF1
IGKJRB
IGKJRB1
KBF2
RBP-J
RBPJK
RBPSUH
SUH
csl
Species Homo sapiens
 Database cross references - RBPJ
ExoCarta ExoCarta_3516
Vesiclepedia VP_3516
Entrez Gene 3516
HGNC 5724
MIM 147183
UniProt Q06330  
 RBPJ identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RBPJ
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IEA
    protein binding GO:0005515 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IDA
    protein N-terminus binding GO:0047485 IEA
    RNA polymerase II repressing transcription factor binding GO:0001103 IPI
    sequence-specific DNA binding GO:0043565 IDA
    transcription factor binding GO:0008134 IPI
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IEA
    DNA binding GO:0003677 TAS
    chromatin binding GO:0003682 IEA
    recombinase activity GO:0000150 NAS
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
Biological Process
    endocardium morphogenesis GO:0003160 ISS
    epithelial to mesenchymal transition GO:0001837 ISS
    positive regulation of ephrin receptor signaling pathway GO:1901189 ISS
    negative regulation of ossification GO:0030279 ISS
    auditory receptor cell fate commitment GO:0009912 IEA
    gene expression GO:0010467 TAS
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISS
    dorsal aorta morphogenesis GO:0035912 ISS
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    cardiac left ventricle morphogenesis GO:0003214 ISS
    Clara cell differentiation GO:0060486 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    angiogenesis GO:0001525 ISS
    blood vessel endothelial cell fate specification GO:0097101 ISS
    somatic stem cell maintenance GO:0035019 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    defense response to bacterium GO:0042742 IEA
    positive regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061419 IDA
    outflow tract morphogenesis GO:0003151 ISS
    positive regulation of cell proliferation involved in heart morphogenesis GO:2000138 ISS
    positive regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment GO:1901297 IEA
    ventricular trabecula myocardium morphogenesis GO:0003222 ISS
    regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation GO:0003256 ISS
    inflammatory response to antigenic stimulus GO:0002437 IEA
    sebaceous gland development GO:0048733 IEA
    humoral immune response GO:0006959 IEA
    positive regulation of ERBB signaling pathway GO:1901186 ISS
    negative regulation of cell proliferation GO:0008285 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    hair follicle maturation GO:0048820 IEA
    secondary heart field specification GO:0003139 IEA
    labyrinthine layer blood vessel development GO:0060716 ISS
    blood vessel remodeling GO:0001974 IEA
    Notch signaling pathway GO:0007219 TAS
    atrioventricular canal development GO:0036302 ISS
    B cell differentiation GO:0030183 IEA
    pituitary gland development GO:0021983 IEA
    positive regulation of transcription of Notch receptor target GO:0007221 IDA
    interleukin-4 secretion GO:0072602 IEA
    regulation of timing of cell differentiation GO:0048505 IEA
    DNA recombination GO:0006310 NAS
    epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 ISS
    blood vessel lumenization GO:0072554 ISS
    Notch signaling involved in heart development GO:0061314 IC
    keratinocyte differentiation GO:0030216 IEA
    epidermal cell fate specification GO:0009957 IEA
    positive regulation of BMP signaling pathway GO:0030513 ISS
    myeloid dendritic cell differentiation GO:0043011 IEA
    arterial endothelial cell fate commitment GO:0060844 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    MAML1-RBP-Jkappa- ICN1 complex GO:0002193 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor complex GO:0005667 IEA
 Experiment description of studies that identified RBPJ in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RBPJ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GCN5L2  
Two-hybrid Homo sapiens
2 SNW1 22938
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 PCAF  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 CIR  
Two-hybrid Homo sapiens
5 SPEN 23013
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 SND1 27044
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 NOTCH1 4851
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 SIN3A  
Reconstituted Complex Homo sapiens
9 NOTCH2 4853
Two-hybrid Homo sapiens
10 NCOR2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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